Abstract:
BACKGROUND:Near-infrared spectroscopy is proposed as a method for providing real-time urea concentrations during hemodialysis treatments. The feasibility of such noninvasive urea measurements is evaluated in undiluted dialysate fluid. METHODS:Near-infrared spectra were collected from calibration solutions of urea prepared in dialysate fluid. Spectra were collected over three distinct spectral regions, and partial least-squares calibration models were optimized and compared for each. Selectivity for urea was demonstrated with two-component samples composed of urea and glucose in the dialysate matrix. The clinical significance of this approach was assessed by measuring urea in real hemodialysate samples. RESULTS:Urea absorptions within the combination and short-wavelength, near-infrared spectral regions provided sufficient spectral information for sound calibration models in the dialysate matrix. The combination spectral region had SEs of calibration (SEC) and prediction (SEP) of 0.38 mmol/L and 0.26 mmol/L, respectively, over the 4720-4600 cm(-1) spectral range with 5 partial least-square factors. A second calibration model was established over the combination region from a series of solutions prepared with independently variable concentrations of urea and glucose. The best calibration model for urea in the presence of variable glucose concentrations had a SEC of 0.6 mmol/L and a SEP of 0.4 mmol/L for a 5-factor model over the 4600-4350 cm(-1) spectral range. There was no significant decrease in SEP when the 4720-4600 cm(-1) calibration model was used to measure urea in real samples collected during actual hemodialysis. CONCLUSIONS:Urea can be determined with sufficient sensitivity and selectivity for clinical measurements within the matrix of the hemodialysis fluid.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Eddy CV,Arnold MAsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
1279-86issue
7eissn
0009-9147issn
1530-8561journal_volume
47pub_type
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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doi:
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doi:
更新日期:1988-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1990-07-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1978-11-01 00:00:00
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doi:
更新日期:1987-03-01 00:00:00
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doi:
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更新日期:1994-06-01 00:00:00
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doi:
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更新日期:2020-09-01 00:00:00
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