Evidence-based laboratory medicine in oncology drug development: from biomarkers to diagnostics.

abstract：BACKGROUND:The recent interest of clinical laboratories in developing serum testosterone assays based on isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) stems from the lack of accuracy of direct immunoassays. In this study, we assessed the accuracy and state of standardization (traceabilit...

journal_title：Clinical chemistry

authors： Thienpont LM,Van Uytfanghe K,Blincko S,Ramsay CS,Xie H,Doss RC,Keevil BG,Owen LJ,Rockwood AL,Kushnir MM,Chun KY,Chandler DW,Field HP,Sluss PM

更新日期：2008-08-01 00:00:00

abstract：BACKGROUND:Evidence suggests that systemic inflammation may adversely impact HDL function. In this study we sought to evaluate the independent and incremental predictive performance of GlycA-a novel serum inflammatory biomarker that is an aggregate measure of enzymatically glycosylated acute phase proteins-and HDL subc...

journal_title：Clinical chemistry

authors： McGarrah RW,Kelly JP,Craig DM,Haynes C,Jessee RC,Huffman KM,Kraus WE,Shah SH

更新日期：2017-01-01 00:00:00

abstract：:This method for determining aluminum in human serum involves electrothermal atomic absorption spectrophotometry. A longer drying time allows less pre-dilution of the sample, and the method also includes a flush cycle after atomization. Standard-addition methodology is used to eliminate matrix effects and the need for ...

journal_title：Clinical chemistry

authors： Bertholf RL,Brown S,Renoe BW,Wills MR,Savory J

更新日期：1983-06-01 00:00:00

abstract：:We have improved the protocol for RNA quantification by using RNase protection. Instead of precipitation and extraction with phenol and chloroform, we use a faster and more reliable precipitation based on guanidinium thiocyanate (GdSCN). The internal standard is produced by in vitro transcription of a DNA template con...

journal_title：Clinical chemistry

authors： Petersen NE,Larsen LK,Nissen H,Jensen LG,Jensen A,Hyltoft Petersen P,Hørder,Gregersen N,Kristiansen K

更新日期：1995-11-01 00:00:00

abstract：BACKGROUND:The analysis of bodily fluids using SELDI-TOF MS has been reported to identify signatures of spectral peaks that can be used to differentiate patients with a specific disease from normal or control patients. This report is the 2nd of 2 companion articles describing a validation study of a SELDI-TOF MS approa...

journal_title：Clinical chemistry

authors： McLerran D,Grizzle WE,Feng Z,Thompson IM,Bigbee WL,Cazares LH,Chan DW,Dahlgren J,Diaz J,Kagan J,Lin DW,Malik G,Oelschlager D,Partin A,Randolph TW,Sokoll L,Srivastava S,Srivastava S,Thornquist M,Troyer D,Wright GL

更新日期：2008-01-01 00:00:00

abstract：:The S-peptide and S-protein fragments of ribonuclease S (RNase S, no EC no. assigned) have been immobilized onto separate Sepharose gels via a "leash" of polycytidylic acid substrate. Each of these gels releases its RNase fragment when treated with the complementary enzyme fragment or with RNase A (EC 3.1.27.5), and t...

journal_title：Clinical chemistry

authors： Ehrat M,Cecchini DJ,Giese RW

更新日期：1986-09-01 00:00:00

abstract：:We describe an automated enzymatic procedure for quantifying creatine in erythrocytes. In this assay, after sample clean-up, creatine kinase (CK; EC 2.7.3.2) and pyruvate kinase (EC 2.1.7.40) are used as auxiliary enzymes; lactate dehydrogenase (EC 1.1.1.27) is used in the indicator reaction. CK is also used as the st...

journal_title：Clinical chemistry

authors： Beyer C,Alting IH

更新日期：1996-02-01 00:00:00

abstract：:Humans are exposed daily to low concentrations of many different chemical substances, natural and some man-made. Although many of these substances can be toxic at high levels, typical exposures are far below the effect levels. The responses produced by man-made aromatic hydrocarbon receptor agonists, such as dioxins, ...

journal_title：Clinical chemistry

authors： Silkworth JB,Brown JF Jr

更新日期：1996-08-01 00:00:00

abstract：:Using results from patients, we studied several methods for long-term (years) monitoring of the analytical stability of various chemistry tests. For each test, we obtained on a bimonthly basis histograms of > or = 200 to approximately 2000 data points and determined the median, mean, and mean of those data points in t...

journal_title：Clinical chemistry

authors： Lott JA,Smith DA,Mitchell LC,Moeschberger ML

更新日期：1996-06-01 00:00:00

abstract：:Genetic engineering of beta-galactosidase (EC 3.2.1.23) has led to the development of a new homogeneous assay system, CEDIA. The Z gene of the lac operon of Escherichia coli encodes a large enzymatically inactive polypeptide that spontaneously aggregates and folds to form active beta-galactosidase. Using recombinant D...

journal_title：Clinical chemistry

authors： Henderson DR,Friedman SB,Harris JD,Manning WB,Zoccoli MA

更新日期：1986-09-01 00:00:00

abstract：:We describe the influence of autoantibodies that bind creatine kinase BB (CK-BB) on the methods for MB isoenzyme. If these autoantibodies are present in patients' sera, they cause the formation of macro CK type 1 (immunoglobulin-linked CK-BB). In some of these cases they can bind not only endogenous CK-BB but also CK-...

journal_title：Clinical chemistry

authors： Stein W,Bohner J

更新日期：1985-07-01 00:00:00

abstract：BACKGROUND:Two mutations in HFE, G845A (amino acid substitution C282Y) and C187G (H63D), are associated with hereditary hemochromatosis. We developed and validated a novel method, linked linear amplification (LLA), for detection of these two mutations. METHODS:Two segments of HFE were amplified by a multiplex LLA reac...

journal_title：Clinical chemistry

authors： Killeen AA,Breneman JW 3rd,Carillo AR,Ugozzoli LA,Lowery JD,Liu J,Hixson CS

更新日期：2003-07-01 00:00:00

abstract：:We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for ...

journal_title：Clinical chemistry

authors： Lindbäck B,Bergman A

更新日期：1989-05-01 00:00:00

abstract：BACKGROUND:Monogenic autoinflammatory diseases are caused by pathogenic variants in genes that regulate innate immune responses, and are characterized by sterile systemic inflammatory episodes. Since symptoms can overlap within this rapidly expanding disease category, accurate genetic diagnosis is of the utmost importa...

journal_title：Clinical chemistry

authors： Shinar Y,Ceccherini I,Rowczenio D,Aksentijevich I,Arostegui J,Ben-Chétrit E,Boursier G,Gattorno M,Hayrapetyan H,Ida H,Kanazawa N,Lachmann HJ,Mensa-Vilaro A,Nishikomori R,Oberkanins C,Obici L,Ohara O,Ozen S,Sarkisian T

更新日期：2020-04-01 00:00:00

abstract：:In this simple and low-cost radioimmunoassay (RIA) procedure for thyroxin (T4), ethacridine (6,9-diamino-2-ethoxyacridine lactate; Rivanol) is used to separate antibody-bound ligand and free ligand. This acridine dye precipitates free T4 not bound to antibody. At pH 8.6, it precipitates more than 90% of 125I-labeled T...

journal_title：Clinical chemistry

authors： Bhupal V,Mani RS

更新日期：1983-11-01 00:00:00

abstract：:Hepatitis A virus (HAV) infection occurs worldwide and is an important cause of acute viral hepatitis in the US. In this review, I cover the epidemiology, course of infection, clinical manifestations, serological responses, and prevention of this infection. Although most patients completely recover from this disease, ...

journal_title：Clinical chemistry

authors： Lemon SM

更新日期：1997-08-01 00:00:00

abstract：BACKGROUND:Standardization of hemoglobin (Hb)A(1c) measurements is a process of considerable interest for quality assurance in diabetes management. To contribute to continuous quality improvement and fulfillment of the requirements for reference measurement procedures according to the standards of the International Org...

journal_title：Clinical chemistry

authors： Kaiser P,Akerboom T,Ohlendorf R,Reinauer H

更新日期：2010-05-01 00:00:00

abstract：:Imferon (Merrell-National), an iron-dextran complex, is widely used in patients with iron deficiency. It is present in the circulation in appreciable amounts for two to three weeks after administration and interferes with all tested colorimetric iron assays, both with and without deproteinization. The amount of the pl...

journal_title：Clinical chemistry

authors： Huisman W

更新日期：1980-04-01 00:00:00

abstract：BACKGROUND:Methylmalonic acid (MMA) in plasma or serum is widely used for assessment of vitamin B(12) status. However, data are sparse regarding factors, besides renal function, that may influence MMA concentrations. We searched for important determinants of plasma MMA in the general population. METHODS:In 6946 middle...

journal_title：Clinical chemistry

authors： Vogiatzoglou A,Oulhaj A,Smith AD,Nurk E,Drevon CA,Ueland PM,Vollset SE,Tell GS,Refsum H

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:Standardization of clinical measurements of C-reactive protein (CRP) is based on the availability of reference materials. The serum protein reference material ERM-DA470 was used as a calibrant for various commercially available immunoassays but has now been exhausted. The recently released ERM-DA470k/IFCC wa...

journal_title：Clinical chemistry

authors： Rzychon M,Zegers I,Schimmel H

更新日期：2010-09-01 00:00:00

abstract：:We present data on a filter-paper collection and assay method for measurement of glycated hemoglobin (gHb). Onto filter paper dipped into a solution of glucose oxidase (EC 1.1.3.4) and allowed to dry, approximately 20 microL of capillary blood was spotted. For analysis, we eluted the dried blood spot from the paper by...

journal_title：Clinical chemistry

authors： Little RR,McKenzie EM,Wiedmeyer HM,England JD,Goldstein DE

更新日期：1986-05-01 00:00:00

abstract：:Methods for measuring thyroid autoantibodies--to thyroperoxidase (TPOAb), thyroglobulin (TgAb), and thyrotropin receptor (TRAb)--have improved over the last decade, but increasingly, accurate and sensitive methods are needed for identifying patients with autoimmune thyroid diseases and individuals at high risk for ons...

journal_title：Clinical chemistry

authors： Feldt-Rasmussen U

更新日期：1996-01-01 00:00:00

abstract：:Measured triglyceride concentrations were extremely low (less than 100 mg/L) in the serum of some patients who were receiving hydroxyurea for myeloproliferative diseases. The assay being used to quantify triglycerides was a "cascaded" enzymatic method involving (a) lipase, to generate glycerol from triglycerides; (b) ...

journal_title：Clinical chemistry

authors： McPherson RA,Brown KD,Agarwal RP,Threatte GA,Jacobson RJ

更新日期：1985-08-01 00:00:00

abstract：:We report the first solid-phase radioimmunoassay for triiodothyronine with use of antibody-coated tubes, which is designed specifically for the fully automated radioimmunoassay instrument, Micromedic Systems' "Concept 4 Automatic Radioassay." Antisera to triiodothyropropionic acid/bovine serum albumin were raised in r...

journal_title：Clinical chemistry

authors： Rugg NE,Hasler MJ,Bjornsen RE,Painter K

更新日期：1977-05-01 00:00:00

abstract：BACKGROUND:Diagnosis of mitochondrial disorders usually requires a muscle biopsy to examine mitochondrial function. We describe our diagnostic procedure and results for 29 patients with mitochondrial disorders. METHODS:Muscle biopsies were from 43 healthy individuals and 29 patients with defects in one of the oxidativ...

journal_title：Clinical chemistry

authors： Janssen AJ,Trijbels FJ,Sengers RC,Wintjes LT,Ruitenbeek W,Smeitink JA,Morava E,van Engelen BG,van den Heuvel LP,Rodenburg RJ

更新日期：2006-05-01 00:00:00

abstract：:We describe a modified tantalum ribbon atomic absorption procedure for determining lead in undiluted whole blood. An instrumentation Laboratory (I.L.) Model 151 atomic absorption spectrophotometer equipped with an I.L. Model 355 Flameless Sampler was used. The Flameless Sampler was slightly modified to include three-c...

journal_title：Clinical chemistry

authors： Therrell BL Jr,Drosche JM,Dziuk TW

更新日期：1978-07-01 00:00:00

abstract：:We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic pro...

journal_title：Clinical chemistry

authors： Patel PC,Aubin L,Côte J

更新日期：1986-10-01 00:00:00

abstract：:Methods are described for detection of lactate dehydrogenase (LDH) inhibitors in preparations of reduced nicotinamide adenine dinucleotide. They are (a) comparison of values by kinetic methods with those measured for highly purified NADH and (b) examination of Lineweaver-Burk plots. Chromatographic inhomogeneities are...

journal_title：Clinical chemistry

authors： Howell BF,Margolis S,Schaffer R

更新日期：1976-10-01 00:00:00

abstract：BACKGROUND:The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. METHODS:Re...

journal_title：Clinical chemistry

authors： Ståhlberg A,Thomsen C,Ruff D,Åman P

更新日期：2012-12-01 00:00:00

abstract：BACKGROUND:Calcitonin (CT) is a sensitive marker for evaluation of medullary thyroid cancer (MTC). However, CT measurement can vary with assay- and nonassay-dependent factors, and procalcitonin (PCT) measurement has been proposed for evaluating questionable increases in CT. METHODS:We tested 2 fully automated CT assay...

journal_title：Clinical chemistry

authors： Kratzsch J,Petzold A,Raue F,Reinhardt W,Bröcker-Preuss M,Görges R,Mann K,Karges W,Morgenthaler N,Luster M,Reiners C,Thiery J,Dralle H,Fuhrer D

更新日期：2011-03-01 00:00:00