Abstract:
BACKGROUND:Standardization of hemoglobin (Hb)A(1c) measurements is a process of considerable interest for quality assurance in diabetes management. To contribute to continuous quality improvement and fulfillment of the requirements for reference measurement procedures according to the standards of the International Organization for Standardization, we developed a calibration system of highest metrological order using isotope dilution-mass spectrometry with a reference material. METHOD:Samples were prepared by enzymatic cleavage based on the IFCC reference measurement procedure for LC-MS analysis. After digestion the samples were spiked with [D7]-labeled glycated and nonglycated hexapeptides as internal standards for quantification. LC-MS analysis was performed by using a C12 reversed-phase column and a gradient of acetonitrile/H(2)O containing 0.1% formic acid. RESULTS:Calibration systems for HbA(1c) determination based on liquid chromatography-isotope dilution-mass spectrometry (LC-ID-MS) and on the IFCC reference measurement procedure were compared. A linear regression analysis demonstrated a correlation of r(2) = 1.00 between the 2 different calibration systems. Mean deviation was 5.5% for the calibration and 3.3% for hemolysate samples, with a mean expanded uncertainty of 4.9%. CONCLUSIONS:This LC-ID-MS procedure allows the current IFCC reference measurement procedure for HbA(1c) to be raised to a higher order of accuracy.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Kaiser P,Akerboom T,Ohlendorf R,Reinauer Hdoi
10.1373/clinchem.2009.139477subject
Has Abstractpub_date
2010-05-01 00:00:00pages
750-4issue
5eissn
0009-9147issn
1530-8561pii
clinchem.2009.139477journal_volume
56pub_type
杂志文章abstract::We compared cotinine, carboxyhemoglobin, and thiocyanate concentrations in blood sampled from 187 cigarette smokers and 181 non-smokers. All three differed significantly between smokers and non-smokers. Cotinine performed best as a test for assessing smoking status, with a sensitivity of 98% as compared with 94% for c...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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更新日期:1983-01-01 00:00:00
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更新日期:1996-10-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1992-11-01 00:00:00
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更新日期:1996-04-01 00:00:00
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1989-02-01 00:00:00
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doi:
更新日期:1976-11-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1992-10-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1979-06-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1993-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:2000-02-01 00:00:00
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doi:
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journal_title:Clinical chemistry
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doi:
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doi:
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更新日期:1975-03-01 00:00:00
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doi:
更新日期:1996-08-01 00:00:00
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更新日期:1981-06-01 00:00:00
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