Abstract：
:Haptoglobin is a hemoglobin-binding protein expressed by a genetic polymorphism as three major phenotypes: 1-1, 2-1, and 2-2. Most attention has been paid to determining haptoglobin phenotype as a genetic fingerprint used in forensic medicine. More recently, several functional differences between haptoglobin phenotypes have been demonstrated that appear to have important biological and clinical consequences. Haptoglobin polymorphism is associated with the prevalence and clinical evolution of many inflammatory diseases, including infections, atherosclerosis, and autoimmune disorders. These effects are explained by a phenotype-dependent modulation of oxidative stress and prostaglandin synthesis. Recent evidence is growing that haptoglobin is involved in the immune response as well. The strong genetic pressure favoring the 2-2 phenotype suggests an important role of haptoglobin in human pathology.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Langlois MR,Delanghe JRsubject
Has Abstractpub_date
1996-10-01 00:00:00pages
1589-600issue
10eissn
0009-9147issn
1530-8561journal_volume
42pub_type
杂志文章,评审abstract：:We evaluated the EMIT (enzyme-multiplied immuno technique) and FPIA (fluorescence polarization immunoassay) urine screening systems for detection of benzodiazepine intake. Healthy male volunteers were given single oral therapeutic doses of alprazolam (2 mg), chlordiazepoxide (25 mg), flunitrazepam (1 mg), lorazepam (3...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:The most popular pretreatment method of plasma samples for the measurement of ascorbate (AsA) and dehydroascorbate (DHA) has been an acidic deproteinization via metaphosphoric acid or trichloroacetic acid. In general, DHA is absent in plasma samples prepared from human blood in a conventional manner. However, when the...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:A polymorphism in the human prostate-specific glandular kallikrein (hKLK2) gene was described by direct sequencing (by PCR) of genomic DNAs isolated from prostatic cancer tissue, benign prostatic hyperplasia tissue, and blood leukocyte specimens. Results showed two forms of human prostate-specific glandular kallikrein...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：INTRODUCTION:Commercially available SARS-CoV-2 serological assays based on different viral antigens have been approved for the qualitative determination of anti-SARS-CoV-2 antibodies. However, there are limited published data associating the results from commercial assays with neutralizing antibodies. METHODS:67 speci...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1093/clinchem/hvaa211
更新日期：2020-09-07 00:00:00
abstract：:We report results for adjusted ionized calcium (at pH 7.4) and actual ionized calcium (at actual pH) in capillary blood from 183 patients with disorders of calcium metabolism (primary hyperparathyroidism, secondary hyperparathyroidism of malabsorption, primary hypoparathyroidism, Paget's disease, acromegaly, hypercalc...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:A fixed-time incubation method for measuring acetylsalicylic acid esterase was improved by using a higher concentration of the substrate, acetylsalicylic acid, and an activator, Ca2+. The enzymatic activity in serum was 25-fold that measured by earlier methods. Inhibition studies showed a pattern similar to that repor...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：BACKGROUND:Few data compare cardiac troponin T (cTnT) and cardiac troponin I (cTnI) in a general population. We sought to evaluate the distribution and association between cTnT, cTnI, and cardiovascular risk factors in a large general population cohort. METHODS:High-sensitivity cTnT and cTnI were measured in serum fro...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2018.292086
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:Concomitant quantification of multiple mutant KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) alleles may provide information in addition to that provided by standard mutation-detection procedures. We assessed the feasibility of a nanofluidic digital PCR array platform to detect and quantify KRAS...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2012.186577
更新日期：2012-09-01 00:00:00
abstract：:A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：:Numerous outcome measures can be used to characterize and compare the performance of alternative quality-control (QC) strategies. The performance measure traditionally used in the QC planning process is the probability of rejecting an analytical run when a critical out-of-control error condition exists. Another perfor...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Using a high-affinity solid-phase-bound antibody (Ka = 1.2 X 10(11) L/mol), we have standardized a two-step radioimmunoassay for free triiodothyronine (FT3) in serum, based on immunoextraction. The method was validated by comparison with an equilibrium-dialysis procedure (r = 0.96) involving RIA of T3 in the dialysate...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：BACKGROUND:Insulin, growth hormone (GH), and growth factors (insulin-like growth factors [IGFs] and their binding proteins [IGFBPs]) are known to influence fetal growth and also the synthesis/secretion of the recently discovered hormones leptin and ghrelin. METHODS:In 153 delivering mothers and their offspring at birt...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.095299
更新日期：2008-03-01 00:00:00
abstract：:We describe conditions for determining carboxypeptidase N (EC 3.4.17.3) activity by liquid chromatography. Serum (10 microL) is mixed with the artificial substrates hippuryl-L-arginine (30 mmol/L) and hippuryl-L-lysine (100 mmol/L) in 50 mmol/L 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:We report the intermediate-term effects of three consecutive evenings of moderate ethanol ingestion (0.75 g/kg body weight each evening) on activity values for alkaline phosphatase, gamma-glutamyltransferase, creatine kinase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in sera of ni...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:We have developed a series of photochemical silver stains that are more than 100 times as sensitive as the Coomassie Blue stains. These stains facilitate the quantitative estimation of proteins in two-dimensional gel patterns. Recently, we discovered that by using light (photodevelopment) to catalyze the reduction of ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：BACKGROUND:The susceptibility of plasma lipids to oxidation is thought to be a factor contributing to atherogenic risk. Various groups have studied the in vitro oxidizability of isolated LDL and examined the effects of conventional antioxidants. The drawbacks associated with the isolation of LDL for evaluation of in vi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.051078
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:Direct equilibrium dialysis and direct ultrafiltration free thyroxine (T(4)) assays rely on semipermeable membranes to exclude T(4)-binding serum proteins from dialysates and ultrafiltrates. The presence of these proteins in dialysates or ultrafiltrates will yield spuriously high free T(4) values when free T...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.085316
更新日期：2007-05-01 00:00:00
abstract：:Both cellulose acetate electrophoresis and citrate agar electrophoresis were performed on 834 blood samples collected on filter paper in Jamaica and shipped for testing to the National Hemoglobinopathy Standardization Laboratory at the U.S. National Center for Disease Control. Additionally, 30 blood samples collected ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:Cholinesterase activity in human serum was measured calorimetrically and compared to values obtained with the spectrophotometric method of Rappaport et al. [Clin. Chim. Acta 4, 227 (1959)]. The response of the calorimeter upon mixing serum with acetylcholine corresponded to the cholinesterase activity of the serum, th...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-10-01 00:00:00
abstract：BACKGROUND:False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation dif...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.091488
更新日期：2007-12-01 00:00:00
abstract：:A new method for quantifying specific amino acids in small volumes of plasma and whole blood has been developed. Based on isotope-dilution tandem mass spectrometry, the method takes only a few minutes to perform and requires minimal sample preparation. The accurate assay of both phenylalanine and tyrosine in dried blo...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The Arg-to-Trp substitution at codon 3500 in the apolipoprotein (apo) B-100 gene is established as a cause of familial defective apo B-100 (FDB), a functional mutation, resulting in reduced LDL receptor binding and manifest hypercholesterolemia. In a search for similar mutations in 163 Malaysians, we screened the puta...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:We compared different sample-handling techniques for measurement of Na+ and K+ with ion-selective electrodes (ISE). Imprecision was less for venous blood (with a minimum of heparin) than for plasma, serum, or capillary blood. The results for K+ were higher for serum than for whole blood, and higher for whole blood tha...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:We have developed an interactive statistical quality-control system for the small- to medium-sized radioimmunoassay laboratory, which can be used in a programmable desk-top calculator instead of the medium- or large-scale computer systems usually required. The design of this quality-control system is modeled after the...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:In this assay for lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 (LD-1), 1,6-hexanediol is added to serum after total LD has been measured. After incubation for 5 min the total LD remaining is determined. Samples from patients who had a myocardial infarction or who had undergone bypass surgery were assayed simultaneo...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamina...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:We examined the changes in sensitivity and specificity that would occur with alterations in the sample-collection schedule and (or) cutoff criterion for the increase in hydrogen concentration in breath after administration of doses of lactose in the dietary range. In a breath-analysis test to classify individuals as l...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:We have developed and validated simple, rapid immunoassays to measure concentrations of albumin in urine ranging from 5 to 200 mg/L. We use antiserum to human albumin, raised in sheep, and we separate the antibody-bound and free fractions of albumin by using a second antiserum, produced in donkeys, against the Fc frag...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：BACKGROUND:Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g., RHD or Y-chromosome-specific sequences. To date, the analysis of other fetal geneti...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2003.029835
更新日期：2004-06-01 00:00:00
abstract：BACKGROUND:The recent development of nonradioactive automated assays for serum parathyroid hormone (PTH) and 25-hydroxyvitamin D (25OHD) has made measurement of these two hormones possible in many laboratories. In this study, we compared two new assays for PTH and 25OHD adapted on an automated analyzer, the LIAISON, wi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2004.037606
更新日期：2005-02-01 00:00:00
