Oxidative susceptibility of unfractionated serum or plasma: response to antioxidants in vitro and to antioxidant supplementation.

abstract：:We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct ...

journal_title：Clinical chemistry

authors： Peter F,Growcock G,Strunc G

更新日期：1978-09-01 00:00:00

abstract：:Lactate dehydrogenase (LD) isoenzymes 1 and 2 in human serum were separated on a column of diethylaminoethyl-Sephadex. Samples layered on mini-columns were eluted with buffered sodium chloride (100, 150, and 200 mmol/liter). Lactate dehydrogenase activity in column effluents was measured by the Wacker method, and thei...

journal_title：Clinical chemistry

authors： Mercer DW

更新日期：1978-03-01 00:00:00

abstract：BACKGROUND:The hepatocyte-derived acute-phase reactant C-reactive protein (CRP) has been the subject of intense research over the last 2 decades for its possible role in the pathogenesis of cardiovascular diseases. This research has spawned interest in the use of the blood concentration of CRP for predicting a first co...

journal_title：Clinical chemistry

authors： Hingorani AD,Shah T,Casas JP,Humphries SE,Talmud PJ

更新日期：2009-02-01 00:00:00

abstract：BACKGROUND:Growth differentiation factor 15 (GDF15), a stress-responsive cytokine produced in cardiovascular cells under conditions of inflammation and oxidative stress, is emerging as an important prognostic marker in individuals with and without existing cardiovascular disease (CVD). We therefore examined the clinica...

journal_title：Clinical chemistry

authors： Ho JE,Mahajan A,Chen MH,Larson MG,McCabe EL,Ghorbani A,Cheng S,Johnson AD,Lindgren CM,Kempf T,Lind L,Ingelsson E,Vasan RS,Januzzi J,Wollert KC,Morris AP,Wang TJ

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:Urinary cross-linked N-telopeptide of type I collagen (NTX) is a reliable bone resorption marker in patients with metabolic bone disease. We assessed a clinically available serum NTX assay suitable for anuric patients on hemodialysis (HD). METHODS:Serum concentrations of NTX, C-terminal telopeptide of type ...

journal_title：Clinical chemistry

authors： Maeno Y,Inaba M,Okuno S,Yamakawa T,Ishimura E,Nishizawa Y

更新日期：2005-12-01 00:00:00

abstract：:We modified the liquid-chromatographic assay of Schleicher and Wieland (J Clin Chem Clin Biochem 1981; 19:81-7) for measuring lysine-bound glucose in serum proteins, increasing its performance and practicality. After precipitating serum proteins from 10- to 50-microL samples with ethanol (700 mL/L) and hydrolyzing the...

journal_title：Clinical chemistry

authors： Drexel H,Klocker H,Patsch JR,Breier C,Braunsteiner H

更新日期：1987-09-01 00:00:00

abstract：BACKGROUND:C-Reactive protein (CRP) has been shown to be a strong predictor of coronary heart disease (CHD) and is being considered in cardiovascular disease risk assessment. The number of normolipidemic individuals who are eligible for evaluation of CRP in overall CHD risk assessment is not known. METHODS:We analyzed...

journal_title：Clinical chemistry

authors： Ajani UA,Ford ES,Mokdad AH

更新日期：2004-09-01 00:00:00

abstract：:We have developed a simple, specific, and sensitive reversed-phase liquid-chromatographic method for accurate and simultaneous analysis of corticosterone and cortisol in human plasma. We achieved a detection limit of 300 ng/L for both steroids by modifying the old solid-phase extraction method to make use of "Tef Elut...

journal_title：Clinical chemistry

authors： Hariharan M,Naga S,VanNoord T,Kindt EK

更新日期：1992-03-01 00:00:00

abstract：:We measured thyroid hormones and thyroxin-binding proteins in serum from 62 normal lactating and 52 nonlactating women at three months postpartum, and compared these values with those for 42 nonpregnant control women of similar age. Mean thyroxin concentrations in the lactating and nonlactating women were significantl...

journal_title：Clinical chemistry

authors： Iwatani Y,Amino N,Tanizawa O,Mori H,Kawashima M,Yabu Y,Miyai K

更新日期：1987-07-01 00:00:00

abstract：:A new method for quantifying specific amino acids in small volumes of plasma and whole blood has been developed. Based on isotope-dilution tandem mass spectrometry, the method takes only a few minutes to perform and requires minimal sample preparation. The accurate assay of both phenylalanine and tyrosine in dried blo...

journal_title：Clinical chemistry

authors： Chace DH,Millington DS,Terada N,Kahler SG,Roe CR,Hofman LF

更新日期：1993-01-01 00:00:00

abstract：:Risk can lead to catastrophe. Risk-management systems are highly effective in preventing the catastrophes of fire, earthquakes, and work-site injuries. No such effective systems are present to prevent health and social problems. A practical, cost-effective system to manage risk in children is being developed by the no...

journal_title：Clinical chemistry

authors： Friedman GM

更新日期：1992-08-01 00:00:00

abstract：:In this automated system for assaying guanidino compounds, the compounds are resolved on an ion-exchange (Dowex 50) column, then reacted with phenanthrenequinone in alkaline solution. This mixture is then acidified and the resulting fluorescence is measured (lambda ex, 305 nm; lambda em, 395 nm). I applied the system ...

journal_title：Clinical chemistry

authors： Natelson S

更新日期：1984-02-01 00:00:00

abstract：:To evaluate their potential usefulness in the differential diagnosis of Cushing's syndrome, we estimated the urinary excretion rates of the following non-metabolized, unbound steroid hormones: pregnenolone, progesterone, 17-OH-pregnenolone, 17-OH-progesterone, dehydroepiandrosterone (DHEA), androstenedione, testostero...

journal_title：Clinical chemistry

authors： Schöneshöfer M,Weber B,Oelkers W,Nahoul K,Mantero F

更新日期：1986-01-01 00:00:00

abstract：BACKGROUND:Different technologies, such as quantitative real-time PCR or microarrays, have been developed to measure microRNA (miRNA) expression levels. Quantification of miRNA transcripts implicates data normalization using endogenous and exogenous reference genes for data correction. However, there is no consensus ab...

journal_title：Clinical chemistry

authors： Schwarzenbach H,da Silva AM,Calin G,Pantel K

更新日期：2015-11-01 00:00:00

abstract：:Estimation of plasma catecholamine concentrations by "high-performance" liquid chromatography, although increasingly used, still presents difficulties. We describe a method for clean-up of plasma samples on cation-exchange resin before they are extracted with alumina. This procedure eliminates many of the interfering ...

journal_title：Clinical chemistry

authors： Frayn KN,Maycock PF

更新日期：1983-07-01 00:00:00

abstract：:A dissociation-enhanced lanthanide fluoroimmunoassay of serum cortisol based on time-resolved fluorescence is described. The assay is a direct assay, where cortisol immobilized on the wall of a microtiter-strip well competes with cortisol in the sample for the europium-labeled polyclonal antibody. The amount of bound ...

journal_title：Clinical chemistry

authors： Eskola JU,Näntö V,Meurling L,Lövgren TN

更新日期：1985-10-01 00:00:00

abstract：:By using bivariate probability estimation for the diagnosis of acute myocardial infarction (AMI) we show how to overcome the difficulties encountered for patients whose clinical presentation is atypical and those encountered when multiple isoenzyme determinations are treated by univariate methods. We use the values fo...

journal_title：Clinical chemistry

authors： Bernstein LH,Good IJ,Holtzman GI,Deaton ML,Babb J

更新日期：1989-03-01 00:00:00

abstract：BACKGROUND:An abnormal IgLkappa:IgLlambda ratio has long been used as a clinical criterion for non-Hodgkin B-cell lymphomas. As a first step toward a quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed a method for determination of IgLkappa:IgLlambda ratio in clinical sample...

journal_title：Clinical chemistry

authors： Ståhlberg A,Aman P,Ridell B,Mostad P,Kubista M

更新日期：2003-01-01 00:00:00

abstract：:In this method for separating glycosylated from nonglycosylated hemoglobin in blood by electrophoresis on cellulose acetate membranes, we exploit the affinity of low-molecular-mass dextran sulfate for the nonglycosylated fraction, which increases the mobility of the latter relative to that of glycosylated hemoglobin. ...

journal_title：Clinical chemistry

authors： Ambler J,Janik B,Walker G

更新日期：1983-02-01 00:00:00

abstract：:We have developed an interactive statistical quality-control system for the small- to medium-sized radioimmunoassay laboratory, which can be used in a programmable desk-top calculator instead of the medium- or large-scale computer systems usually required. The design of this quality-control system is modeled after the...

journal_title：Clinical chemistry

authors： Cernosek SF Jr,Gutierrez-Cernosek RM

更新日期：1978-07-01 00:00:00

abstract：:We compared a stabilized-temperature L'vov platform furnace containing an end-heated graphite atomizer (HGA) and transverse Zeeman background-correction system with a side-heated furnace system (transversely heated graphite atomizer; THGA) containing a longitudinal Zeeman background-correction system for the determina...

journal_title：Clinical chemistry

authors： Bradley C,Leung FY

更新日期：1994-03-01 00:00:00

abstract：:We measured advanced glycosylation end products in the mesenteric artery of 37 patients (ages 29-82 years), 34 of whom were nondiabetic. Samples of arterial tissue were obtained during bowel resectioning. Advanced glycosylation end products were measured as collagen-linked fluorescence (excitation wavelength 370 nm, e...

journal_title：Clinical chemistry

authors： MacDonald E,Lee WK,Hepburn S,Bell J,Scott PJ,Dominiczak MH

更新日期：1992-04-01 00:00:00

abstract：BACKGROUND:First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years. The widespread adoption of this isothermal molecular tool in many diagnostic fields represents an affordable (approxim...

journal_title：Clinical chemistry

authors： Daher RK,Stewart G,Boissinot M,Bergeron MG

更新日期：2016-07-01 00:00:00

abstract：:We assayed norepinephrine and epinephrine by utilizing solvent extraction, fluorescence derivatization, and "high-performance" liquid chromatography. A 100-microL aliquot of urine was extracted twice according to Smedes et al. (J Chromatogr 1982;231:25-39) and subsequently incubated with 1,2-diphenylethylenediamine. A...

journal_title：Clinical chemistry

authors： Moleman P,van Dijk J

更新日期：1990-05-01 00:00:00

abstract：BACKGROUND:PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response in bone marrow (BM) and peripheral blood (PB). PHOX2B has been identified as a sensitive and specific MRD marker; however, its expression varies between tumors....

journal_title：Clinical chemistry

authors： Stutterheim J,Gerritsen A,Zappeij-Kannegieter L,Yalcin B,Dee R,van Noesel MM,Berthold F,Versteeg R,Caron HN,van der Schoot CE,Tytgat GA

更新日期：2009-07-01 00:00:00

abstract：BACKGROUND:The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. METHODS:Re...

journal_title：Clinical chemistry

authors： Ståhlberg A,Thomsen C,Ruff D,Åman P

更新日期：2012-12-01 00:00:00

abstract：:We report the kinetics of the reactions of unconjugated bilirubin and conjugated bilirubin with p-diazobenzene sulfonic acid in aqueous media. Our studies confirm that each reaction proceeds in two steps and that the second step is catalyzed by sulfanilic acid. In the presence of an excess of p-diazobenzenesulfonic ac...

journal_title：Clinical chemistry

authors： Landis JB,Pardue HL

更新日期：1978-10-01 00:00:00

abstract：:We present a comparative evaluation of two commercial kits, the "Quantitope" and "GammaCoat," for radioimmunoassay of digoxin in serum. These kits, in which iodine-125 is used as a label, proved to suitable for digoxin assay as determined by their reproducibility, sensitivity, precision, and a regression analysis. Hem...

journal_title：Clinical chemistry

authors： Kuczala ZJ,Ahluwalia GS

更新日期：1976-02-01 00:00:00

abstract：:Creatine kinase isoenzyme BB (CK-BB) is found in the serum of patients with various types of cancer. Using both radioimmunoassay and agarose electrophoresis, we found abnormal amounts of this isoenzyme in the serum of 15 of 17 patients with untreated prostatic carcinoma. Among patients with other types of adenocarcino...

journal_title：Clinical chemistry

authors： Silverman LM,Dermer GB,Zweig MH,Van Steirteghem AC,Tökés ZA

更新日期：1979-08-01 00:00:00

abstract：BACKGROUND:Cystic fibrosis is a life-threatening genetic disorder that has been associated with mutations in the CFTR [cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)] gene. Hundreds of CFTR mutations have been detected to date. Current CFTR genotyping assays target a s...

journal_title：Clinical chemistry

authors： Abou Tayoun AN,Tunkey CD,Pugh TJ,Ross T,Shah M,Lee CC,Harkins TT,Wells WA,Tafe LJ,Amos CI,Tsongalis GJ

更新日期：2013-10-01 00:00:00