Abstract：
:We coupled a kinetic procedure to a selective inhibiting method for determining amylase isoenzymes in biological samples, using 4-nitrophenylmaltopentaoside plus 4-nitrophenylmaltohexaoside as substrate and a wheat-germ selective inhibitor with the Gilford S-III spectrophotometer. On plotting remaining amylase activities/total amylase activities (R/T) vs pancreatic amylase activities/salivary amylase activities (P/S) ratios, we found the curve to be linear for P/S ratios from 0.2 to 5. The inhibition rate of amylase inhibitor was constant in solutions having total amylase activities between 20 and (at least) 900 U/L. CVs were 3.1 to 7.1% for pancreatic amylase and 2.0 to 12.9% for salivary amylase in serum. Correlation with the Phadebas method was excellent (r = 0.99) for both pancreatic and salivary amylase. We also automated this procedure in an Hitachi 705 analyzer and correlated the results (r = 0.99) with those by our manual method.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Jiménez A,Arenas J,Santos I,Martínez Asubject
Has Abstractpub_date
1986-08-01 00:00:00pages
1577-80issue
8eissn
0009-9147issn
1530-8561journal_volume
32pub_type
杂志文章abstract：BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.132480
更新日期：2010-03-01 00:00:00
abstract：:To evaluate their potential usefulness in the differential diagnosis of Cushing's syndrome, we estimated the urinary excretion rates of the following non-metabolized, unbound steroid hormones: pregnenolone, progesterone, 17-OH-pregnenolone, 17-OH-progesterone, dehydroepiandrosterone (DHEA), androstenedione, testostero...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Clinicians experience difficulty in correctly interpreting the results of in vitro thyroid function tests in the presence of abnormalities of thyrobinding proteins or when results are borderline. This difficulty has been largely resolved in our laboratory by three innovations. First, the borderline areas for each of t...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：BACKGROUND:Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary refere...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2016.256768
更新日期：2016-07-01 00:00:00
abstract：:We describe a rapid method for determining cholesterol sulfate in plasma and erythrocytes. After its single-step isolation by means of anion-exchange chromatography cholesterol sulfate is hydrolyzed, trimethylsilylated, and determined by gas chromatography with flame ionization detection. 5 beta-Cholestan-3 alpha-ol s...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:We evaluated the Hybritech Tandem-E procedure for quantifying choriogonadotropin (hCG) in human serum. In this "sandwich"-type assay, two monoclonal antibodies directed against different regions of the hCG molecule are used, one coated on a plastic bead, the second conjugated to alkaline phosphatase. The assay can det...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensit...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：:Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:We adapted an inhibition assay for methotrexate, involving dihydrofolate reductase from bovine liver, for use with a discrete analyzer (the ABA-100). The analyzer was used both for dilution and a 5-min pre-incubation of the sample with NADPH--enzyme reagent, and for the assay itself. The standard curve was linear betw...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
abstract：:In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-12-01 00:00:00
abstract：:We measured thyroid hormones and thyroxin-binding proteins in serum from 62 normal lactating and 52 nonlactating women at three months postpartum, and compared these values with those for 42 nonpregnant control women of similar age. Mean thyroxin concentrations in the lactating and nonlactating women were significantl...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Major discoveries have been made in the past few years on the structure and mode of biosynthesis of serum albumin. The complete amino acid sequence of this protein has been determined, and its covalent structure shown to be a single peptide chain grouped into a series of nine disulfide-bonded loops. These loops appear...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1977-01-01 00:00:00
abstract：:We describe a rapid, precise, and sensitive radiometric assay for human lactoferrin. In this typical "sandwich"-type assay, anti-human lactoferrin is adsorbed onto a polystyrene sphere and bound lactoferrin is detected by the subsequent binding of 125I-labeled anti-human lactoferrin. The assay is accurate for lactofer...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:The location of the Reference Value for an analyte within the population distribution affects the magnitude of error due to methodological bias. Using the gaussian distribution, we evaluated the effects of systematic and proportional biases of the method (positive and negative), mean value, and standard deviation on t...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:In this report on trace-element concentrations (As, Ca, Cd, Cl, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Rb, Sb, Se, Zn) in human heart, liver, kidney, aorta, and rib obtained from 200 autopsied patients, we give special attention to sampling procedure, analysis technique, and various sources of error (autolysis...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：BACKGROUND:Little is known about trace metal alterations in the bones of dialysis patients or whether particular types of renal osteodystrophy are associated with either increased or decreased skeletal concentrations of trace elements. Because these patients are at risk for alterations of trace elements as well as for ...
journal_title：Clinical chemistry
pub_type： 杂志文章,多中心研究
doi：
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:Measurement of serum thyroglobulin (Tg) is used to monitor patients after treatment for differentiated thyroid carcinoma (TC). Difficulty in using Tg as a biomarker of the recurrence of TC in many patients stems from the presence of endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immuno...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2012.195594
更新日期：2013-06-01 00:00:00
abstract：:We describe a radioassay for cobalamin (vitamin B12) in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is rele...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：BACKGROUND:D-2-Hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) are poorly understood organic acidurias. To investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes, we measured disease-...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/49.7.1133
更新日期：2003-07-01 00:00:00
abstract：:The increasing use of monoclonal antibodies (MAbs) for disease diagnosis and therapy has created a class of patients at risk for systematic error in clinical testing due to interference by human anti-murine antibodies (HAMA). HAMA interference is often difficult to detect and can cause either an increase or a decrease...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:A method has been developed for the determination of inorganic arsenic [As(III) and As(V)] and its organic metabolites (monomethylarsenic and dimethylarsenic) in urine by flow-injection hydride generation atomic absorption spectrometry. The nontoxic seafood-derived arsenobetaine and arsenocholine species were first se...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：BACKGROUND:It is important that cardiac troponin be measured accurately with a robust method to limit false results with potentially adverse clinical outcomes. In this study, we characterized the robustness of 4 analytical platforms by measuring the outlier rate between duplicate results. METHODS:We measured cardiac t...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2010.159830
更新日期：2011-05-01 00:00:00
abstract：:I determined the in vitro biological half-lives or decay constants for creatine kinase isoenzymes at various temperatures. Values at 37 degrees C are consistent with values reported by others in vivo, which suggests that in vivo irreversible inactivation is primarily thermal. Reversible inactivation appears to be an o...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：BACKGROUND:The analytical and clinical performance of homogeneous LDL-cholesterol assays has been reported, but their reactions with subfractions of LDL and VLDL have not been described in detail. METHODS:We evaluated reaction selectivity of two homogeneous LDL-cholesterol assays, LDLk (Kyowa Medex) and LDLd (Daiichi ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:We studied the effects on 25 analytes of duration of contact of serum with non-anticoagulated blood and of temperature. Serum was separated after blood was allowed to stand, for 0, 2, 4, 6, 8, 24, or 48 h at 4, 23, or 30 degrees C. Results obtained for bilirubin, albumin, zinc sulfate turbidity, thymol turbidity, chol...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Highly purified human lactate dehydrogenase 1 has been used in an interlaboratory evaluation and improvement progran in clinical chemistry in New York State since 1971. Although there are difficulties in determining and assigning the most nearly accurate values for test samples in the absence of a reference method and...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：BACKGROUND:Dried blood spots (DBS) are a convenient and inexpensive method for biobanking. Although many countries have established population-based DBS biobanks from neonatal screening programs, the quality and usefulness of DNA from DBS have not been extensively assessed. METHODS:We compared 4 common DNA extraction ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.087510
更新日期：2007-08-01 00:00:00
abstract：:Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Biochemical markers of bone turnover can provide prognostic information about the risk of osteoporotic fracture and are useful tools for monitoring efficacy of antiresorptive therapy. A serum-based automated assay may be of better clinical value than urinary markers because of lower imprecision and day-to-da...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:We describe a "high-performance" liquid-chromatographic assay for measuring ritalinic acid in 300 muL of serum. The procedure includes adsorption of ritalinic acid and internal standard (alpha,alpha-dimethyl-beta-emthylsuccinimide) from serum onto charcoal, from which both compounds are eluted with methanol. The metha...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
