Abstract:
BACKGROUND:Standardization of clinical measurements of C-reactive protein (CRP) is based on the availability of reference materials. The serum protein reference material ERM-DA470 was used as a calibrant for various commercially available immunoassays but has now been exhausted. The recently released ERM-DA470k/IFCC was intended to fully replace ERM-DA470. However, the new material was not suited for the certification of CRP because of a bias introduced by the lyophilization process that caused loss of about 20% of CRP measurable by routine immunoassays, compared with the nonlyophilized material that was stored in a liquid frozen state. METHODS:We investigated the physicochemical state of CRP in a set of 4 lyophilized and 2 nonlyophilized serum-based CRP-containing materials by semi-native gel electrophoresis, Western blotting, and gel filtration. RESULTS:We detected a monomeric form of CRP (mCRP) in lyophilized materials at a concentration significantly higher than seen in the materials not subjected to lyophilization. Different reconstitution protocols led to variations of the monomeric CRP fraction found in reconstituted, previously lyophilized material. CONCLUSIONS:Most of the 20% loss in measured CRP after lyophilization of the material can be accounted for by the dissociation of natively pentameric CRP into subunits. The observed dissociation results from lyophilization and subsequent reconstitution of the material at insufficient concentration levels of calcium ions. In the presence of various protein forms, differences in antibody specificity and reactivity between immunoassays and alterations of stoichiometry of antigen-antibody interactions can contribute to the divergence of the measured values.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Rzychon M,Zegers I,Schimmel Hdoi
10.1373/clinchem.2010.147124subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1475-82issue
9eissn
0009-9147issn
1530-8561pii
clinchem.2010.147124journal_volume
56pub_type
杂志文章abstract::A new method (enzyme-ligand immunoassay, ELIA) is described for the estimation of estrogen (ER) and progesterone (PR) receptors in microsamples of human breast cancer tissue. The technique, based on the nonisotopic measurement of receptor-bound estradiol and progesterone, involves three steps: (a) simultaneous saturat...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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abstract::9-(2-Phosphonylmethoxyethyl)adenine (PMEA), (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA), and (R,S)-9-(3-fluoro-2-phosphonylmethoxypropyl)adenine (FPMPA) are selective antiviral agents with activity against a broad spectrum of DNA viruses and retroviruses. A highly sensitive method has been developed to ...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1996-12-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1976-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1993-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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doi:
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doi:
更新日期:1975-04-01 00:00:00
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更新日期:2018-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1977-02-01 00:00:00
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更新日期:2014-04-01 00:00:00
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journal_title:Clinical chemistry
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doi:10.1373/clinchem.2012.186866
更新日期:2012-08-01 00:00:00
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