Abstract：
BACKGROUND:Hepcidin-25 reduces iron absorption by binding to the intestinal iron transporter ferroportin and causing its degradation. Currently, little is known about the basal regulation of circulating hepcidin-25. In addition, although erythropoietin administration has been reported to decrease the circulating hepcidin concentration, information is limited regarding how other stimulators of erythropoiesis, such as growth hormone (GH), might alter hepcidin-25 concentrations. METHODS:We used a sensitive and specific hepcidin-25 dual-monoclonal antibody sandwich immunoassay to measure hepcidin-25 in healthy human volunteers at various time points throughout the day and during 3 days of fasting and subsequent refeeding. We also measured hepcidin-25 concentrations in healthy volunteers after GH administration. RESULTS:In healthy individuals, hepcidin-25 concentrations displayed a diurnal variation, with concentrations being lowest in the early morning and steadily increasing throughout the day before declining during the evening hours, a pattern that was not influenced by food intake. Prolonged fasting produced statistically significant increases in hepcidin-25 concentrations. Refeeding reversed this process, and GH administration markedly decreased hepcidin-25 concentrations. CONCLUSIONS:Our results indicate that in humans, hepcidin-25 exhibits diurnal changes that can be altered by prolonged fasting, which increases hepcidin-25 concentrations approximately 3-fold after 3 days of fasting, possibly owing to a suppression of erythropoiesis that may occur during the fasting state to preserve tissue iron concentrations. In contrast, GH administration decreased hepcidin-25 concentrations by approximately 65%, presumably by stimulating erythropoiesis. These results indicate that circulating hepcidin-25 concentrations display much more dynamic and rapid variation than might have been anticipated previously.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Troutt JS,Rudling M,Persson L,Ståhle L,Angelin B,Butterfield AM,Schade AE,Cao G,Konrad RJdoi
10.1373/clinchem.2012.186866subject
Has Abstractpub_date
2012-08-01 00:00:00pages
1225-32issue
8eissn
0009-9147issn
1530-8561pii
clinchem.2012.186866journal_volume
58pub_type
杂志文章abstract：BACKGROUND:Plasma creatinine concentration and calculated creatinine clearance are of limited value as glomerular filtration rate (GFR) markers in patients with decompensated liver cirrhosis. We assessed plasma cystatin C as an indicator of GFR in such patients. METHODS:We studied 36 patients with decompensated liver ...
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journal_title：Clinical chemistry
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doi：
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doi：
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doi：
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doi：
更新日期：1979-12-01 00:00:00
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doi：
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doi：
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doi：
更新日期：1991-11-01 00:00:00
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doi：
更新日期：1995-10-01 00:00:00
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doi：
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doi：
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doi：
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doi：
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更新日期：1995-08-01 00:00:00
