Liquid-chromatographic measurement of taurine in cerebrospinal fluid of normal individuals and patients with meningitis.

abstract：BACKGROUND:Efforts to reduce the high global prevalence of nutritional anemia require the use of both reliable laboratory assays to distinguish iron deficiency from other causes of anemia and cost-effective methods for collection of blood specimens under field conditions. The suitability of using small plasma samples s...

journal_title：Clinical chemistry

authors： Flowers CH,Cook JD

更新日期：1999-10-01 00:00:00

abstract：:We present data on a filter-paper collection and assay method for measurement of glycated hemoglobin (gHb). Onto filter paper dipped into a solution of glucose oxidase (EC 1.1.3.4) and allowed to dry, approximately 20 microL of capillary blood was spotted. For analysis, we eluted the dried blood spot from the paper by...

journal_title：Clinical chemistry

authors： Little RR,McKenzie EM,Wiedmeyer HM,England JD,Goldstein DE

更新日期：1986-05-01 00:00:00

abstract：BACKGROUND:Mutations in the retina-specific ABC transporter (ABCA4) gene have been associated with several forms of macular degenerations. Because the high complexity of the molecular genotype makes scanning of the ABCA4 gene cumbersome, we describe here the first use of denaturing HPLC (DHPLC) to screen for ABCA4 muta...

journal_title：Clinical chemistry

authors： Stenirri S,Fermo I,Battistella S,Galbiati S,Soriani N,Paroni R,Manitto MP,Martina E,Brancato R,Allikmets R,Ferrari M,Cremonesi L

更新日期：2004-08-01 00:00:00

abstract：:We evaluated the Reflotron (Boehringer Mannheim) system for measuring activities of alanine aminotransferase (EC 2.6.1.2) and gamma-glutamyltransferase (EC 2.3.2.2). Within- and between-day precision were comparable with conventional analytical methods. The linear range of the methods extended to more than 40-fold the...

journal_title：Clinical chemistry

authors： James DR,Price CP

更新日期：1987-06-01 00:00:00

abstract：:Ion chromatography is a new analytical technique in which the ions to be analyzed are separated on a special pellicular ion-exchange column. Once separated, the effluent from the separating column passes into a second ion-exchange column, which removes the eluent or buffer ions from the moving phase without removing t...

journal_title：Clinical chemistry

authors： Anderson C

更新日期：1976-09-01 00:00:00

abstract：:We evaluated a newly developed solid-phase immunoassay (EIA) of terminal deoxynucleotidyl transferase (TdT, EC 2.7.7.3) and compared it with the enzymatic assay of TdT involving DNA polymerase. We assessed the precision, performance characteristics, and clinical efficacy of the EIA procedure, using 249 specimens of pe...

journal_title：Clinical chemistry

authors： Fleisher M,Stankievic R,Schwartz D,Schwartz MK

更新日期：1987-02-01 00:00:00

abstract：BACKGROUND:Immune repertoire sequencing of the T-cell receptor can identify clonotypes that have expanded as a result of antigen recognition or hematological malignancies. However, current sequencing protocols display limitations with nonuniform amplification and polymerase-induced errors during sequencing. Here, we de...

journal_title：Clinical chemistry

authors： Johansson G,Kaltak M,Rîmniceanu C,Singh AK,Lycke J,Malmeström C,Hühn M,Vaarala O,Cardell S,Ståhlberg A

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:The determination of cellular beta-galactocerebrosidase activity is an established procedure to diagnose Krabbe disease and monitor the efficacy of gene/stem cell-based therapeutic approaches aimed at restoring defective enzymatic activity in patients or disease models. Current biochemical assays for beta-ga...

journal_title：Clinical chemistry

authors： Martino S,Tiribuzi R,Tortori A,Conti D,Visigalli I,Lattanzi A,Biffi A,Gritti A,Orlacchio A

更新日期：2009-03-01 00:00:00

abstract：:We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for ...

journal_title：Clinical chemistry

authors： Lindbäck B,Bergman A

更新日期：1989-05-01 00:00:00

abstract：:We examined the relationship in a large-living population between the concentration of triglycerides in plasma from fasting persons and the concentration of very-low-density lipoprotein cholesterol (VLDL-C) as determined after preparative ultracentrifugation. Data from individuals in the Framingham Heart Study who wer...

journal_title：Clinical chemistry

authors： Wilson PW,Abbott RD,Garrison RJ,Castelli WP

更新日期：1981-12-01 00:00:00

abstract：BACKGROUND:Prostate-specific antigen (PSA) occurs in serum both free and in complex with protease inhibitors. The complex with alpha1-antichymotrypsin (ACT) is the major form in serum, and the proportion of PSA-ACT is higher in prostate cancer (PCa) than in benign prostatic hyperplasia (BPH). PSA also forms a complex w...

journal_title：Clinical chemistry

authors： Zhang WM,Finne P,Leinonen J,Vesalainen S,Nordling S,Stenman UH

更新日期：1999-06-01 00:00:00

abstract：BACKGROUND:Although cardiac troponin (cTn) is a cornerstone marker in the assessment and management of patients with acute coronary syndrome (ACS) and heart failure (HF), cTn is not diagnostically specific for any single myocardial disease process. This narrative review discusses increases in cTn that result from acute...

journal_title：Clinical chemistry

authors： Kelley WE,Januzzi JL,Christenson RH

更新日期：2009-12-01 00:00:00

abstract：:We investigated the effects on plasma osteocalcin concentrations of different anticoagulants used to collect the blood samples. Plasma osteocalcin concentrations measured by enzyme immunoassay and radioimmunoassay are influenced by the nature of the anticoagulants used. The most significant difference between concentr...

journal_title：Clinical chemistry

authors： Power MJ,O'Dwyer B,Breen E,Fottrell PF

更新日期：1991-02-01 00:00:00

abstract：BACKGROUND:The promise of targeted therapies in molecularly defined subsets of cancer has led to a transformation of the process of drug development in oncology. To target cancer successfully and precisely requires high-quality translational data. Such data can be generated by the use of biomarkers that answer key ques...

journal_title：Clinical chemistry

authors： Modur V,Hailman E,Barrett JC

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response in bone marrow (BM) and peripheral blood (PB). PHOX2B has been identified as a sensitive and specific MRD marker; however, its expression varies between tumors....

journal_title：Clinical chemistry

authors： Stutterheim J,Gerritsen A,Zappeij-Kannegieter L,Yalcin B,Dee R,van Noesel MM,Berthold F,Versteeg R,Caron HN,van der Schoot CE,Tytgat GA

更新日期：2009-07-01 00:00:00

abstract：:We report the intermediate-term effects of three consecutive evenings of moderate ethanol ingestion (0.75 g/kg body weight each evening) on activity values for alkaline phosphatase, gamma-glutamyltransferase, creatine kinase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in sera of ni...

journal_title：Clinical chemistry

authors： Freer DE,Statland BE

更新日期：1977-05-01 00:00:00

abstract：:The time-course of the contamination of exogenous hydrogen from cigarette smoke on postprandial breath hydrogen concentration was evaluated in 10 subjects, six regular smokers and four occasional smokers. Breath hydrogen values were determined by gas chromatography 10 min, 5 min, and immediately prior to smoking a fil...

journal_title：Clinical chemistry

authors： Rosenthal A,Solomons NW

更新日期：1983-11-01 00:00:00

abstract：BACKGROUND:Plasma concentrations of the peptides midregional proadrenomedullin (MR-proADM), midregional proatrial natriuretic peptide (MR-proANP), and C-terminal endothelin-1 precursor fragment (CT-proET-1) are increased in various cardiovascular conditions. However, there is limited information about the association a...

journal_title：Clinical chemistry

authors： Kollerits B,Sturm G,Lamina C,Hammerer-Lercher A,Rantner B,Stadler M,Ziera T,Struck J,Klein-Weigel P,Fraedrich G,Kronenberg F

更新日期：2013-04-01 00:00:00

abstract：:We report a method for the extraction of angiotensin peptides from plasma with a mixture of acetone, 1 mol/L HCl, and water (40/1/5 by vol). The method is highly reproducible for the measurement of angiotensin I and angiotensin II in small sample volumes, with analytical recoveries of about 80% for both peptides. We i...

journal_title：Clinical chemistry

authors： Hermann K,Ganten D,Unger T,Bayer C,Lang RE

更新日期：1988-06-01 00:00:00

abstract：BACKGROUND:Astilbin is a flavonoid isolated from the rhizome of Smilax glabra. In a previous study, we revealed its unique immunosuppressive activity, a selective inhibition against activated T lymphocytes. This characteristic of astilbin is beneficial for the treatment of human immune diseases. METHODS:We incubated a...

journal_title：Clinical chemistry

authors： Guo J,Qian F,Li J,Xu Q,Chen T

更新日期：2007-03-01 00:00:00

abstract：BACKGROUND:An assay using a mouse antisialyl Lewis X (sLeX) antibody (CSLEX-1) is used clinically for screening and monitoring patients with breast cancer in Japan. However, the IgM isoform of CSLEX-1 is not preferred for the assay because the bulkiness of IgM generally causes poor accessibility to the antigen. To solv...

journal_title：Clinical chemistry

authors： Yamashita J,Kobayashi I,Tatematsu K,Sezutsu H,Noda K,Ishihara H

更新日期：2016-11-01 00:00:00

abstract：:We are developing a method for quantifying plasma lipoproteins by proton nuclear magnetic resonance (NMR) spectroscopy that offers advantages over existing clinical methods. We showed that the major lipoproteins have distinct NMR properties sufficient to permit their concentrations to be extracted from a computer line...

journal_title：Clinical chemistry

authors： Otvos JD,Jeyarajah EJ,Bennett DW,Krauss RM

更新日期：1992-09-01 00:00:00

abstract：:In this method for separating glycosylated from nonglycosylated hemoglobin in blood by electrophoresis on cellulose acetate membranes, we exploit the affinity of low-molecular-mass dextran sulfate for the nonglycosylated fraction, which increases the mobility of the latter relative to that of glycosylated hemoglobin. ...

journal_title：Clinical chemistry

authors： Ambler J,Janik B,Walker G

更新日期：1983-02-01 00:00:00

abstract：:A three-parameter model for directly estimating imprecision profiles from replicated immunoassay results is compared with a six-parameter indirect profile model obtained by the usual method of combining error in the raw response measurements with the slope of the standard curve. Direct estimation is likely to be less ...

journal_title：Clinical chemistry

authors： Sadler WA,Smith MH,Legge HM

更新日期：1988-06-01 00:00:00

abstract：:A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include...

journal_title：Clinical chemistry

authors： Bais R,Edwards JB

更新日期：1976-12-01 00:00:00

abstract：:Endotoxin, the lipopolysaccharide cell wall constituent of Gram-negative bacteria, produces symptoms of the Gram-negative sepsis syndrome. By measuring endotoxin in blood from septic patients it may be possible to select a subpopulation of patients in which mortality can be prevented by treatment with anti-endotoxin a...

journal_title：Clinical chemistry

authors： Salden HJ,Bas BM

更新日期：1994-08-01 00:00:00

abstract：:Intravascular monitoring of arterial blood gases provides the means to detect precipitous change in cardiopulmonary function almost instantaneously, promising major improvement in the ability to manage critically ill patients. Attempts to miniaturize and repackage conventional electrodes for continuous intravascular u...

journal_title：Clinical chemistry

authors： Halbert SA

更新日期：1990-08-01 00:00:00

abstract：BACKGROUND:Formation of deposits of the insoluble amyloid beta-peptide is believed to be causally related with neurodegeneration in Alzheimer disease (AD). The beta-peptide originates from a larger amyloid precursor protein (APP) by the action of proteolytic enzymes. The first proteolytic event leading to amyloid forma...

journal_title：Clinical chemistry

authors： Verheijen JH,Huisman LG,van Lent N,Neumann U,Paganetti P,Hack CE,Bouwman F,Lindeman J,Bollen EL,Hanemaaijer R

更新日期：2006-06-01 00:00:00

abstract：BACKGROUND:Δ(9)-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), and cannabinol (CBN) were measured in breath following controlled cannabis smoking to characterize the time course and windows of detection of breath cannabinoids. METHODS:Exhaled breath was collected from chronic (≥4 times per week) and occas...

journal_title：Clinical chemistry

authors： Himes SK,Scheidweiler KB,Beck O,Gorelick DA,Desrosiers NA,Huestis MA

更新日期：2013-12-01 00:00:00

abstract：:We developed a highly sensitive, convenient assay for measuring blocking-type anti-acetylcholine receptor antibodies, which inhibit the binding of 125I-labeled alpha-bungarotoxin (alpha-BuTx) to the acetylcholine receptor (AChR). This procedure detected inhibitory activities in sera from 76% of patients with myastheni...

journal_title：Clinical chemistry

authors： Hara H,Hayashi K,Ohta K,Itoh N,Nishitani H,Ohta M

更新日期：1993-10-01 00:00:00