Abstract:
:We are developing a method for quantifying plasma lipoproteins by proton nuclear magnetic resonance (NMR) spectroscopy that offers advantages over existing clinical methods. We showed that the major lipoproteins have distinct NMR properties sufficient to permit their concentrations to be extracted from a computer lineshape analysis of the plasma lipid methyl resonance envelope (Clin Chem 1991; 37:377-86). We have now discovered that the spectra of the subspecies within each lipoprotein class are different enough to influence the composite spectrum of that class and hence the spectrum of whole plasma. By including spectra representative of these subspecies as additional components in the lineshape-fitting analysis, a complete concentration profile of very-low-density, low-density (LDL), and high-density (HDL) lipoproteins plus the subspecies distributions within these classes can be simultaneously generated. A pilot study of 30 plasma samples of widely varied composition demonstrated good agreement between NMR-derived values and lipoprotein lipid concentrations and LDL and HDL subspecies distributions determined by gradient-gel electrophoresis.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Otvos JD,Jeyarajah EJ,Bennett DW,Krauss RMsubject
Has Abstractpub_date
1992-09-01 00:00:00pages
1632-8issue
9eissn
0009-9147issn
1530-8561journal_volume
38pub_type
杂志文章abstract::We developed a solid-phase two-site immunoenzymometric assay (IEMA) of the estrogen-induced 52-kDa cathepsin D (EC 3.4.23.5) and its processed forms (48-kDa and 34-kDa proteins) in cytosols of breast cancer tissues, using two monoclonal antibodies directed against two different epitopes of these antigens. The first an...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:In vitro and animal studies suggest that tumor necrosis factor alpha (TNF-alpha) modulates intestinal iron transport. We hypothesized that the effect of TNF-alpha might be particularly relevant if iron absorption is not effectively controlled by the HFE gene. METHODS:In patients with homozygous C282Y hemoch...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2005.065417
更新日期:2006-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::We have developed a fully enzymatic method to measure 1,5 anhydro-D-glucitol (1,5-AG) in serum through use of pyranose oxidase (PROD: EC 1.1.3.10), glucokinase (EC 2.7.1.2), and an ATP-regenerating system. In a previous report (Clin Chem 1989;35:2039-43) the glucose interfering with the measurement of 1,5-AG was remov...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::We report the intermediate-term effects of three consecutive evenings of moderate ethanol ingestion (0.75 g/kg body weight each evening) on activity values for alkaline phosphatase, gamma-glutamyltransferase, creatine kinase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in sera of ni...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
abstract::We describe an automated assay of glycohemoglobin performed with the Abbott Vision analyzer. The assay is based on batch affinity-extraction with 3-aminophenylboronic acid-derivatized agarose beads. Reagents are packaged in a disposable test pack. Whole-blood specimens are hemolyzed with saponin within a glass capilla...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:10.1373/clinchem.2004.034868
更新日期:2004-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::We report microchromatographic measurement of fetal hemoglobin (HbF) proportions in a 36-year-old African-American multigravida woman. At 34 weeks she delivered a 630-g male infant who subsequently did well. Hemoglobin electrophoresis of the hemolysate revealed nearly 100% HbF without HbA, an extremely unusual natural...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::To produce bispecific antibodies (BiAbs) for enzyme immunoassay (EIA) to replace antibody-enzyme conjugates, we developed a panel of 8-azaguanine/ouabain-resistant anti-urease variant hybridoma cell lines for use in hybridoma-hybridoma fusions. These variants represent mouse immunoglobulin subclasses IgM, IgG1, IgG2a,...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::We describe a micro-scale method for determining serum theophylline. The chromatography system includes a muBondapack C18 column and acetonitrile, 70 ml/liter of sodium acetate buffer (10 mmol/liter, ph 4.0) as the mobile phase. Test serum or plasma, 30 mul, is mixed with an equal quantity of a solution containing the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Creatinine and specific gravity (relative density) measurements both allow differences in urine concentration to be taken into account in determining urine cotinine concentrations. In this study we demonstrate that the variance of urine cotinine measurements is reduced comparably when either creatinine or specific gra...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::The clinical value of measuring concentrations of methylmalonic acid in serum (S-MMA) as an aid in the diagnosis of cobalamin deficiency has recently aroused interest. In 58 healthy subjects, ages 40-68 years, we found a 0.95 reference interval of 0.05-0.37 mumol/L (mean 0.21, SD 0.094). In 33 of the subjects, who wer...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract:BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2009.132480
更新日期:2010-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2009.127241
更新日期:2009-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.074021
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::I describe a micro-scale method for determining lead in whole blood by utilizing a graphite furnace. Sample pretreatment consists of fivefold dilution with a dilute surfactant. The method is directly calibrated with lead standards prepared in dilute HNO3. To eliminate a small, nonspecific absorption signal from the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-04-01 00:00:00
abstract::In this enzyme-linked immunoassay for human thyrotropin (TSH) in unextracted serum we use 96-well immunoenzymometric assay plates, first coated with polyclonal antibody to TSH, then incubated with the serum samples and reacted with mouse monoclonal antibody to human TSH. After incubation with alkaline phosphatase-labe...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2011.165704
更新日期:2012-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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