Abstract：
BACKGROUND:Monitoring of the concentration of gentamicin in serum and plasma during therapy is widely recommended and practiced in hospitals. Our aim was to develop a homogeneous immunoassay based on particle-enhanced turbidimetric inhibition immunoassay technology to quantify gentamicin on the Dimension clinical chemistry system. METHODS:Assay performance was assessed on each of the Dimension models in a 15-instrument interlaboratory comparison study. A split-sample comparison (n = 1171) was also performed between the gentamicin methods on the Dimension system and the Abbott TDx analyzer, using multiple reagent and calibrator lots on multiple instruments. RESULTS:The Dimension method was linear to 25.1 micromol/L (12.0 microg/mL) with a detection limit of 0.63 micromol/L (0.3 microg/mL). Calibration was stable for 30 days. The within-run imprecision (CV) was <1.3%, and total imprecision ranged from 1.8% to 3.2% between 4.2 micromol/L (2.0 microg/mL) and 16.7 micromol/L (8.0 microg/mL) gentamicin. Linear regression analysis of the results on the Dimension method (DM) vs the Abbott TDx yielded the following equation: DM = 0.98TDx - 0.42; r = 0.987. Minimal interference was observed from structurally related compounds such as sagamicin, netilmicin, and sisomicin. CONCLUSION:The monoclonal antibody used in this method has similar reactivities toward the individual gentamicin subspecies C1, C1a, and C2, thus providing analytical recovery not significantly dependent on relative subspecies concentrations.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Wei TQ,Chu VP,Craig AR,Duffy JE,Obzansky DM,Kilgore D,Masulli IS,Sanders CM,Thompson JCsubject
Has Abstractpub_date
1999-03-01 00:00:00pages
388-93issue
3eissn
0009-9147issn
1530-8561journal_volume
45pub_type
杂志文章abstract：:Adrenocortical causes of hypertension are established by examining the mineralocorticoid hormones produced in the zona glomerulosa and zona fasciculata. In the zona glomerulosa, aldosterone excess leads to hypertension, hypokalemia, and suppressed plasma renin activity, with increased concentrations of urinary aldoste...
journal_title：Clinical chemistry
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更新日期：1991-10-01 00:00:00
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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更新日期：2000-04-01 00:00:00
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更新日期：2020-05-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1989-02-01 00:00:00
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doi：
更新日期：1982-01-01 00:00:00
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doi：
更新日期：1985-10-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1991-02-01 00:00:00
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doi：
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journal_title：Clinical chemistry
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doi：
更新日期：1983-06-01 00:00:00
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doi：
更新日期：1999-02-01 00:00:00
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journal_title：Clinical chemistry
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doi：
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doi：
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更新日期：1999-10-01 00:00:00
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