Abstract：
:Humans are exposed daily to low concentrations of many different chemical substances, natural and some man-made. Although many of these substances can be toxic at high levels, typical exposures are far below the effect levels. The responses produced by man-made aromatic hydrocarbon receptor agonists, such as dioxins, polychlorinated dibenzofurans, coplanar polychlorinated biphenyls, and polycyclic aromatic hydrocarbons, are also produced, often to greater extents [corrected], by naturally occurring constituents of fried meat, cabbage, broccoli, cauliflower, cocoa, and curry. Our society seems to be concerned about the health risks associated only with the synthetic chemicals, regardless of their proportional contribution to the total agonist activity, and regulates on the basis of such concerns. It would be more protective of the public health to determine acceptable concentrations for each type of response, regardless of the origin of the inducing agent, and issue advisories or regulations accordingly.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Silkworth JB,Brown JF Jrsubject
Has Abstractpub_date
1996-08-01 00:00:00pages
1345-9issue
8 Pt 2eissn
0009-9147issn
1530-8561journal_volume
42pub_type
杂志文章,评审abstract：:9-(2-Phosphonylmethoxyethyl)adenine (PMEA), (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA), and (R,S)-9-(3-fluoro-2-phosphonylmethoxypropyl)adenine (FPMPA) are selective antiviral agents with activity against a broad spectrum of DNA viruses and retroviruses. A highly sensitive method has been developed to ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:We describe a new and specific method for measurement of lecithin palmitic acid in amniotic fluid. Dipalmitoyl lecithin, the major alveolar surfactant, has previously been estimated by measuring the lecithin-sphingomyelin ratio, total lecithin, total phospholipid phosphorus, and (or) total palmitic acid. Our method is...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:We describe a procedure for measuring dialyzable calcium in 60 sera per hour with use of AutoAnalyzer (Technicon) modules. The serum is buffered, then dialyzed in a regular 6-inch (15-cm) dialysis block. A constant proportion of the dialyzable calcium is fluorometrically measured. Analysis of samples containing protei...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:The apolipoprotein A-I (apo A-I) radioimmunoassay established in our laboratory involves use of purified apo A-I as the primary standard for quantifying apo A-I in plasma and a pooled plasma (quality-control sample) as a secondary standard to decrease interassay variation. The measured values for apo A-I increased ove...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:We describe a gas-chromatographic method for measuring chlorpromazine and its metabolites chlorpromazine sulfoxide, mono-N-desmethylchlorpromazine, and di-N-desmethylchlorpromazine at therapeutic concentrations in human serum, with use of a nitrogen detector. The compounds are extracted from serum at pH 10.5 into hexa...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：BACKGROUND:Clinical assessment of vitamin D status often relies on measuring total circulating 25-hydroxyvitamin D3 (25OHD3), but much of each vitamin D metabolite is bound to plasma vitamin D-binding protein (DBP), such that the percentage of free vitamin is very low. We hypothesized that measurement of free rather th...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.059568
更新日期：2006-02-01 00:00:00
abstract：:After extraction of the sample (on filter paper) in the counting vial, cortisol is assayed by adding 50 microL of horse serum containing tritiated cortisol, and 2 mL of toluene scintillator, shaking for 20 min, and counting the radioactivity in a liquid-scintillation counter at ambient temperature. The method can be a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Synovial fluid samples were collected from 45 patients with rheumatoid arthritis, spondylarthropathy, or osteoarthritis, to study their content of elastase (EC 3.4.21.37) and of cysteine proteinases (EC 3.4.22.1, 3.4.22.15). We measured both elastase complexed with alpha 1-proteinase inhibitor and elastase activity to...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：BACKGROUND:We previously reported a family in which the propositus and both her father and paternal grandmother had high serum aminopeptidase N (CD13; EC 3.4.11.2) activity (autosomal dominant). The molecular mass of the serum CD13 polypeptide of the propositus was larger than that of normal CD13, suggesting either a m...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:This HPLC/immunoassay procedure measures digoxin in serum with no interference from digoxin metabolites or digoxin-like factors. We used solid-phase (C18 and Diol) extraction, a C18 column with a tetrahydrofuran/water mobile phase, and final quantification by fluorescence polarization immunoassay. Deslanoside and gito...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:This simple polarization fluoroimmunoassay for detection of amphetamine in urine involves the use of a single reagent: suitably diluted antiserum plus fluorescein-labeled amphetamine as tracer. To this, one adds 10 microL of urine, incubates for a few minutes at room temperature, and measures the fluorescence polariza...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Parathyroid hormone-related protein (PTHrP) was measured in plasma by a new immunoradiometric assay (IRMA) from Nichols Institute. The assay is specific for PTHrP and shows excellent parallelism when measuring keratinocyte fluid, samples with high PTHrP content, and PTHrP-supplemented plasma. A precision profile estab...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:We evaluated the Hybritech Tandem-E procedure for quantifying choriogonadotropin (hCG) in human serum. In this "sandwich"-type assay, two monoclonal antibodies directed against different regions of the hCG molecule are used, one coated on a plastic bead, the second conjugated to alkaline phosphatase. The assay can det...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Mesenteric vascular occlusion and intestinal obstruction are difficult-to-diagnose medical emergencies. We evaluated a large panel of biochemical markers as diagnostic and prognostic indicators in a rat model of intestinal infarction and partial, complete, and strangulated intestinal obstruction. After intestinal infa...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：BACKGROUND:Growth differentiation factor 15 (GDF15), a stress-responsive cytokine produced in cardiovascular cells under conditions of inflammation and oxidative stress, is emerging as an important prognostic marker in individuals with and without existing cardiovascular disease (CVD). We therefore examined the clinica...
journal_title：Clinical chemistry
pub_type： 杂志文章,meta分析
doi：10.1373/clinchem.2012.190322
更新日期：2012-11-01 00:00:00
abstract：:The carboxyl terminal region of corticotropin (ACTH), (Ala34-Phe-Pro-Glu-Leu-Phe39), a region of the hormone conserved during evolution, served as an antigen for the production of a sequence-specific antiserum. In a radioimmunoassay, peptides that extend toward the amino terminal from Ala34, such as [Tyr,Gly]-ACTH34-3...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:A modified agarose electrophoretic system for the separation of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes is described. Bone, liver, high-molecular-mass, and intestinal ALP are separated with high reproducibility. The sensitivity of the agarose system is superior to cellulose acetate in detecting high-Mr ALP. ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:A modified pentamer formation assay (PFA) for quantification of tacrolimus and active metabolites after extraction from whole blood is described. The lower limit of detection was 2 microg/L. Intraassay precision (CV) was 5.7-13.7%, and the interassay CV was 6. 1-14.9%. Tacrolimus trough concentrations in 104 whole blo...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：BACKGROUND:Single-nucleotide polymorphisms (SNPs) in inflammation-related genes have been linked to an increased risk of ischemic stroke. Most of these SNP results have not been replicated, however, and metaanalyses of the effects of inflammation-related genes are rare. We investigated 49 SNPs in 34 genes previously re...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.112151
更新日期：2009-01-01 00:00:00
abstract：:Recombinant DNA techniques are contributing to the understanding of the pathogeneses of genetic, neoplastic, and viral diseases, and are used in the diagnosis of certain genetic and viral diseases. Such techniques will have wider application in the future and will play an increasing role in the clinical laboratory. Th...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：BACKGROUND:Formation of deposits of the insoluble amyloid beta-peptide is believed to be causally related with neurodegeneration in Alzheimer disease (AD). The beta-peptide originates from a larger amyloid precursor protein (APP) by the action of proteolytic enzymes. The first proteolytic event leading to amyloid forma...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2006.066720
更新日期：2006-06-01 00:00:00
abstract：:We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay cor...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Circulating prostate cells can be detected in cancer patients by using reverse transcriptase-PCR (RT-PCR) assay for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) mRNA. A quality-control study involving a conventional RT-PCR assay was performed and, surprisingly, detected both transcripts...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filt...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:The currently available radioimmunoassays of trypsin measure total immunoreactive trypsin (EC 3.1.1.7), which includes both trypsinogen and alpha 1-protease inhibitor-bound trypsin. Hitherto, the only way to differentiate these two forms of trypsin has been to fractionate them on a gel-filtration column. We describe h...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:The accuracy of estimates of blood-alcohol concentration based on measurements of breath-alcohol concentration in a randomly selected subject by a random quantitative evidential breath-alcohol analyzer is evaluated with respect to the breath analyzer itself, its calibration, and the biological variables of the subject...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:The deleterious, disruptive effects of estrogen mimics on the endocrine system were discovered after the compounds were released into the environment. Their chemical structure does not obviously resemble that of steroid hormones; hence, their estrogenic effects were totally unexpected. In addition to occupational expo...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：BACKGROUND:The promise of targeted therapies in molecularly defined subsets of cancer has led to a transformation of the process of drug development in oncology. To target cancer successfully and precisely requires high-quality translational data. Such data can be generated by the use of biomarkers that answer key ques...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：10.1373/clinchem.2012.191072
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Intraventricular hemorrhage (IVH) is a major cause of neurologic disabilities in preterm newborns. We evaluated the use of plasma activin A concentrations to predict the development of perinatal IVH. METHODS:We measured nucleated erythrocyte (NRBC) counts, plasma activin A, hypoxanthine (Hyp), and xanthine ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.065979
更新日期：2006-08-01 00:00:00
abstract：:We report the intermediate-term effects of three consecutive evenings of moderate ethanol ingestion (0.75 g/kg body weight each evening) on activity values for alkaline phosphatase, gamma-glutamyltransferase, creatine kinase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in sera of ni...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
