Abstract:
BACKGROUND:Methylmalonic acid (MMA) in plasma or serum is widely used for assessment of vitamin B(12) status. However, data are sparse regarding factors, besides renal function, that may influence MMA concentrations. We searched for important determinants of plasma MMA in the general population. METHODS:In 6946 middle-aged (47-49 years) and elderly (71-74 years) individuals from the Hordaland Homocysteine Study in Norway, we collected anthropometric measurements, lifestyle data, and plasma MMA, vitamin B(12), and creatinine measurements. For 5820 individuals, we also collected dietary data. RESULTS:Age and plasma creatinine were positively associated with plasma MMA, whereas plasma vitamin B(12) was negatively associated. These variables together with sex were the strongest determinants of plasma MMA, accounting for 16% of the variation (R(2) = 0.16). Addition of anthropometric measures and lifestyle and dietary factors only gave slight improvement (total R(2) = 0.167). Increased plasma MMA was seen when plasma vitamin B(12) was <400 pmol/L. In individuals with vitamin B(12) >or =400 micromol/L (vitamin B(12)-replete), the 2.5th-97.5th percentile reference limits for MMA were 0.10-0.28 micromol/L (middle-aged) and 0.10-0.36 micromol/L (elderly). When plotted against creatinine (nomograms), the 97.5th percentile of MMA was similar in men and women but approximately 0.15 micromol/L higher in elderly than middle-aged individuals. Vitamin B(12)-replete participants had MMA upper limits approximately 0.1 micromol/L (elderly) and 0.04 micromol/L (middle-aged) below those of the unselected population at all creatinine concentrations. CONCLUSIONS:Identified determinants accounted for <17% of the overall variation in plasma MMA. The difference in MMA between middle-aged and elderly individuals is only partly explained by creatinine and vitamin B(12) concentrations.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Vogiatzoglou A,Oulhaj A,Smith AD,Nurk E,Drevon CA,Ueland PM,Vollset SE,Tell GS,Refsum Hdoi
10.1373/clinchem.2009.128678subject
Has Abstractpub_date
2009-12-01 00:00:00pages
2198-206issue
12eissn
0009-9147issn
1530-8561pii
clinchem.2009.128678journal_volume
55pub_type
杂志文章abstract::This procedure for routine quantification of albumin in urine is based on the dye-binding properties of albumin with bromphenol blue. The absorbance of 100 microL of urine mixed with 3 mL of color reagent is measured against blank reagent at 610 nm after 30 s. Results vary linearly with albumin concentration up to 6 g...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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更新日期:1979-04-01 00:00:00
abstract::A modified gas-liquid chromatographic method for determining plasma concentrations of bupivacaine and lidocaine is described, with cyclizine as an internal standard. The extraction procedure requires no solvent evaporation, thus overcoming the problem of drug volatility. Concentrations as low as 0.1 mg/liter can be de...
journal_title:Clinical chemistry
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更新日期:1978-09-01 00:00:00
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更新日期:1986-04-01 00:00:00
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更新日期:1986-04-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1995-06-01 00:00:00
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doi:
更新日期:1994-05-01 00:00:00
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doi:
更新日期:1983-02-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1975-05-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1987-09-01 00:00:00
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doi:10.1373/clinchem.2007.095299
更新日期:2008-03-01 00:00:00
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更新日期:1990-11-01 00:00:00
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