Ultraviolet fluorescence of human sera: I. Sources of characteristic differences in the ultraviolet fluorescence spectra of sera from normal and cancer-bearing humans.

abstract：BACKGROUND:Understanding the distribution of C-reactive protein (CRP) concentration among adult women in the US helps to establish the magnitude of women at increased risk for cardiovascular disease. METHODS:We examined the distribution and correlates of CRP, using data from 2205 women >or=20 years of age from the Nat...

journal_title：Clinical chemistry

authors： Ford ES,Giles WH,Mokdad AH,Myers GL

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND:Evidence suggests that systemic inflammation may adversely impact HDL function. In this study we sought to evaluate the independent and incremental predictive performance of GlycA-a novel serum inflammatory biomarker that is an aggregate measure of enzymatically glycosylated acute phase proteins-and HDL subc...

journal_title：Clinical chemistry

authors： McGarrah RW,Kelly JP,Craig DM,Haynes C,Jessee RC,Huffman KM,Kraus WE,Shah SH

更新日期：2017-01-01 00:00:00

abstract：:A high (2.0 mol/L) NaCl concentration apparently suppresses the secondary-site binding of bilirubin by human serum albumin. Thus if an excess of bilirubin is added to human serum albumin or to neonatal serum in buffer containing 0.1 mol of tris(hydroxymethyl)aminomethane and 2.0 mol of NaCl per liter (pH 7.5), and the...

journal_title：Clinical chemistry

authors： Rutkowski RB

更新日期：1980-01-01 00:00:00

abstract：BACKGROUND:Variability among assays used to measure intact parathyroid hormone (iPTH) is of particular concern because of the routine use of iPTH assay results to guide management of osteodystrophy and calcium metabolism in patients with end-stage renal disease (ESRD). The aim of this study was to determine the extent ...

journal_title：Clinical chemistry

authors： Cantor T,Yang Z,Caraiani N,Ilamathi E

更新日期：2006-09-01 00:00:00

abstract：BACKGROUND:Calcitonin (CT) is a sensitive marker for evaluation of medullary thyroid cancer (MTC). However, CT measurement can vary with assay- and nonassay-dependent factors, and procalcitonin (PCT) measurement has been proposed for evaluating questionable increases in CT. METHODS:We tested 2 fully automated CT assay...

journal_title：Clinical chemistry

authors： Kratzsch J,Petzold A,Raue F,Reinhardt W,Bröcker-Preuss M,Görges R,Mann K,Karges W,Morgenthaler N,Luster M,Reiners C,Thiery J,Dralle H,Fuhrer D

更新日期：2011-03-01 00:00:00

abstract：:A polymorphism in the human prostate-specific glandular kallikrein (hKLK2) gene was described by direct sequencing (by PCR) of genomic DNAs isolated from prostatic cancer tissue, benign prostatic hyperplasia tissue, and blood leukocyte specimens. Results showed two forms of human prostate-specific glandular kallikrein...

journal_title：Clinical chemistry

authors： Herrala A,Kurkela R,Porvari K,Isomäki R,Henttu P,Vihko P

更新日期：1997-02-01 00:00:00

abstract：:In this assay for lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 (LD-1), 1,6-hexanediol is added to serum after total LD has been measured. After incubation for 5 min the total LD remaining is determined. Samples from patients who had a myocardial infarction or who had undergone bypass surgery were assayed simultaneo...

journal_title：Clinical chemistry

authors： Shamberger RJ

更新日期：1987-04-01 00:00:00

abstract：BACKGROUND:Soluble CD146 (sCD146), is an endothelial marker with similar diagnostic power as natriuretic peptides in decompensated heart failure (HF). While natriuretic peptides are released by the failing heart, sCD146 may be released by veins in response to stretch induced by systemic congestion in HF. This study inv...

journal_title：Clinical chemistry

authors： Arrigo M,Truong QA,Onat D,Szymonifka J,Gayat E,Tolppanen H,Sadoune M,Demmer RT,Wong KY,Launay JM,Samuel JL,Cohen-Solal A,Januzzi JL Jr,Singh JP,Colombo PC,Mebazaa A

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND:Small RNAs are key players in the regulation of gene expression and differentiation. However, many different classes of small RNAs (sRNAs) have been described with distinct biogenesis pathways and, as a result, with different biochemical properties. To analyze sRNAs by deep sequencing, complementary DNA synt...

journal_title：Clinical chemistry

authors： Meistertzheim M,Fehlmann T,Drews F,Pirritano M,Gasparoni G,Keller A,Simon M

更新日期：2019-12-01 00:00:00

abstract：BACKGROUND:Improved methods are required to screen drug candidates for their influences on protein interactions. There is also a compelling need for miniaturization of screening assays, with attendant reductions in reagent consumption and assay costs. METHODS:We used sensitive, miniaturized proximity ligation assays (...

journal_title：Clinical chemistry

authors： Gustafsdottir SM,Wennström S,Fredriksson S,Schallmeiner E,Hamilton AD,Sebti SM,Landegren U

更新日期：2008-07-01 00:00:00

abstract：BACKGROUND:Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step. METHODS:We evaluated the sensitivity and specificity of homogeneous scanning, using a saturatin...

journal_title：Clinical chemistry

authors： Reed GH,Wittwer CT

更新日期：2004-10-01 00:00:00

abstract：:Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified ...

journal_title：Clinical chemistry

authors： Welsh JA,Castrén K,Vähäkangas KH

更新日期：1997-12-01 00:00:00

abstract：BACKGROUND:The TDx Fetal Lung Maturity II (FLM II) assay uses amniotic fluid to assess lung maturity of the unborn infant. We investigated common preanalytical factors that influence FLM results, including centrifugation, sample storage, and contamination by whole blood. METHODS:We tested 18 specimens after centrifuga...

journal_title：Clinical chemistry

authors： Grenache DG,Parvin CA,Gronowski AM

更新日期：2003-06-01 00:00:00

abstract：:A profile (chromatographic pattern) of the neutral and acidic metabolites present in cerebrospinal fluid from newborns and infants was obtained by gas-liquid chromatography. The metabolites are those extracted with ethyl acetate and diethyl ether. They are converted to trimethylsilyl ether derivatives, and chromatogra...

journal_title：Clinical chemistry

authors： Malcolm RD,Leonards R

更新日期：1976-05-01 00:00:00

abstract：:This assay was developed to measure apolipoprotein (apo) B in blood samples spotted onto filter paper. The long-term aim is to detect young families with the dominantly inherited familial hypercholesterolemia, during current neonatal screening programs. The interassay CV was 5.6%, and apo B concentrations correlated c...

journal_title：Clinical chemistry

authors： Wang XL,Dudman NP,Wilcken DE

更新日期：1989-06-01 00:00:00

abstract：:We compared different sample-handling techniques for measurement of Na+ and K+ with ion-selective electrodes (ISE). Imprecision was less for venous blood (with a minimum of heparin) than for plasma, serum, or capillary blood. The results for K+ were higher for serum than for whole blood, and higher for whole blood tha...

journal_title：Clinical chemistry

authors： Fogh-Andersen N,Wimberley PD,Thode J,Siggaard-Andersen O

更新日期：1984-03-01 00:00:00

abstract：:Serum amyloid A (SAA), a sensitive acute-phase protein, is the precursor of AA fibrils in reactive amyloidosis. However, SAA is poorly immunogenic, and development and standardization of immunoassays of this protein have been difficult. We established an automated polyclonal/monoclonal microparticle capture enzyme imm...

journal_title：Clinical chemistry

authors： Wilkins J,Gallimore JR,Tennent GA,Hawkins PN,Limburg PC,van Rijswijk MH,Moore EG,Pepys MB

更新日期：1994-07-01 00:00:00

abstract：:We describe a simple fluoroimmunoassay for the determination of thyroxine concentrations in serum. The method, "sequential addition, separation fluoroimmunoassay," involves both thyroxine labeled with fluorescein and magnetizable cellulose/iron oxide particles to which antibodies to thyroxine have been covalently link...

journal_title：Clinical chemistry

authors： Nargessi RD,Ackland J,Hassan M,Forrest GC,Smith DS,Landon J

更新日期：1980-11-01 00:00:00

abstract：:In galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. We describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. Galactose is oxidized by galactose oxidase to D-galacto-hexadialdos...

journal_title：Clinical chemistry

authors： Mason GA,Summer GK,Dutton HH,Schwaner RC Jr

更新日期：1977-06-01 00:00:00

abstract：:In response to the pressures of cost reduction, we established and evaluated a carefully integrated program to deliver clinical laboratory services more promptly and efficiently to the intensive care units (ICUs). The new protocol reduced the steps and turnaround time from ordering tests by physicians to reporting res...

journal_title：Clinical chemistry

authors： Steffes MW,Gillen JL,Fuhrman SA

更新日期：1996-03-01 00:00:00

abstract：BACKGROUND:The differential diagnosis of D-2-hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) can be accomplished only by the measurement of the corresponding 2-hydroxyglutarate (2-HG). Available methods for the determination of D- a...

journal_title：Clinical chemistry

authors： Struys EA,Jansen EE,Verhoeven NM,Jakobs C

更新日期：2004-08-01 00:00:00

abstract：BACKGROUND:The accurate and precise measurement of urinary albumin is critical, since even minor increases are diagnostically sensitive indicators of renal disease, cardiovascular events, and risk for death. To gain insights into potential measurement biases, we systematically compared urine albumin measurements perfor...

journal_title：Clinical chemistry

authors： Shaikh A,Seegmiller JC,Borland TM,Burns BE,Ladwig PM,Singh RJ,Kumar R,Larson TS,Lieske JC

更新日期：2008-09-01 00:00:00

abstract：:The characteristics of the Abbott selective fluorescence polarization immunoassay for cyclosporine in whole blood (TDXSP) were compared with the Incstar radioimmunoassay by using kit standards (INCSP) or TDXSP standards (INCTDX) with samples from adult (48) and pediatric (24) liver-transplant recipients and a further ...

journal_title：Clinical chemistry

authors： Tredger JM,Gonde CE,Williams R

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:Plant-derived cardenolides reportedly possess anticancer properties in human leukemic cells via selective induction of apoptosis, cell cycle arrest, and differentiation. Selective induction of apoptosis with mammalian-derived digoxin-like immunoreactive factor (DLIF) could provide new strategies for anticanc...

journal_title：Clinical chemistry

authors： Ihenetu K,Qazzaz HM,Crespo F,Fernandez-Botran R,Valdes R Jr

更新日期：2007-07-01 00:00:00

abstract：BACKGROUND:Although cardiac troponin (cTn) is a cornerstone marker in the assessment and management of patients with acute coronary syndrome (ACS) and heart failure (HF), cTn is not diagnostically specific for any single myocardial disease process. This narrative review discusses increases in cTn that result from acute...

journal_title：Clinical chemistry

authors： Kelley WE,Januzzi JL,Christenson RH

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:There is extended urinary excretion of Δ(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), and 11-nor-9-carboxy-THC (THCCOOH) in abstinent frequent cannabis smokers. We characterized THC, 11-OH-THC, THCCOOH, cannabidiol, cannabinol, THC-glucuronide, and THCCOOH-glucuronide disposition in urine of fr...

journal_title：Clinical chemistry

authors： Desrosiers NA,Lee D,Concheiro-Guisan M,Scheidweiler KB,Gorelick DA,Huestis MA

更新日期：2014-02-01 00:00:00

abstract：:Using results from patients, we studied several methods for long-term (years) monitoring of the analytical stability of various chemistry tests. For each test, we obtained on a bimonthly basis histograms of > or = 200 to approximately 2000 data points and determined the median, mean, and mean of those data points in t...

journal_title：Clinical chemistry

authors： Lott JA,Smith DA,Mitchell LC,Moeschberger ML

更新日期：1996-06-01 00:00:00

abstract：BACKGROUND:Kallikrein-related peptidase 6 (KLK6), a member of the serine protease family of kallikrein (KLK) genes, is dysregulated in ovarian carcinomas (OCa) and its overexpression is associated with poor prognosis. Regulation of its expression is poorly understood and is likely to be influenced by multiple mechanism...

journal_title：Clinical chemistry

authors： Bayani J,Kuzmanov U,Saraon P,Fung WA,Soosaipillai A,Squire JA,Diamandis EP

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:The term "transrenal DNA" was coined in 2000 to signify that DNA in urine may come from the passage of plasma DNA through the kidney barrier. Although DNA in the urine has the potential to provide a completely noninvasive source of nucleic acids for molecular diagnosis, its existence remains controversial. ...

journal_title：Clinical chemistry

authors： Hung EC,Shing TK,Chim SS,Yeung PC,Chan RW,Chik KW,Lee V,Tsui NB,Li CK,Wong CS,Chiu RW,Lo YM

更新日期：2009-04-01 00:00:00

abstract：BACKGROUND:Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS:Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl...

journal_title：Clinical chemistry

authors： Sakurabayashi I,Watano T,Yonehara S,Ishimaru K,Hirai K,Komori T,Yagi M

更新日期：2003-02-01 00:00:00