Semiautomated spectrophotometry of total phospholipids in plasma.

abstract：:We present a simple method for subclass typing of IgG paraproteins, with which we have demonstrated a large number of paraproteins that were undetected by conventional immunofixation techniques. The types and distribution of IgG subclass paraproteins were analyzed in 92 human sera in which IgG paraproteins had been de...

journal_title：Clinical chemistry

authors： Klouche M,Bradwell AR,Wilhelm D,Kirchner H

更新日期：1995-10-01 00:00:00

abstract：BACKGROUND:To address the continuing need for the standardization of clinical human cardiac troponin I (cTnI) assays, NIST, with the assistance of the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed a new certified reference material, Standard Reference Material (SRM) 2921: Human Cardiac Troponin ...

journal_title：Clinical chemistry

authors： Bunk DM,Welch MJ

更新日期：2006-02-01 00:00:00

abstract：:A new, highly sensitive immunoradiometric thyrotropin (TSH) assay involving solid-phase-coupled monoclonal antibodies (Boots-Celltech Sucrosep IRMA-TSH) has been evaluated in a wide variety of patients with thyroidal and nonthyroidal illnesses and the results compared with those obtained by conventional diagnostic TSH...

journal_title：Clinical chemistry

authors： Bassett F,Eastman CJ,Ma G,Maberly GF,Smith HC

更新日期：1986-03-01 00:00:00

abstract：BACKGROUND:Increased plasma troponin T (cTnT), but not troponin I (cTnI), is frequently observed in end-stage renal failure patients. Although generally considered spurious, we previously reported an associated increased mortality at 12 months. METHODS:We studied long-term outcomes in 244 patients on chronic hemodialy...

journal_title：Clinical chemistry

authors： Ooi DS,Zimmerman D,Graham J,Wells GA

更新日期：2001-03-01 00:00:00

abstract：:We describe an improved separation of the isoenzymes of aspartate aminotransferase (EC 2.6.1.1), based on ion-exchange chromatography. Involving the "Fast Protein Liquid Chromatography" system (Pharmacia) with a MonoQ column, this rapid, reproducible method for quantifying the mitochondrial enzyme shows good resolutio...

journal_title：Clinical chemistry

authors： Grøstad M,Rej R,Huseby NE

更新日期：1990-02-01 00:00:00

abstract：:Imferon (Merrell-National), an iron-dextran complex, is widely used in patients with iron deficiency. It is present in the circulation in appreciable amounts for two to three weeks after administration and interferes with all tested colorimetric iron assays, both with and without deproteinization. The amount of the pl...

journal_title：Clinical chemistry

authors： Huisman W

更新日期：1980-04-01 00:00:00

abstract：BACKGROUND:Identifying novel risk markers in cardiovascular patients remains a research priority. Longer follow-up generally is considered favorable in such studies, but associations of interest may become attenuated with increasing follow-up. This issue has not been adequately addressed in the context of patient cohor...

journal_title：Clinical chemistry

authors： Breitling LP,Mons U,Hahmann H,Koenig W,Rothenbacher D,Brenner H

更新日期：2017-03-01 00:00:00

abstract：:Two-hour postprandial specimens have a -14% proportional bias for lipoprotein(a) [Lp(a)], a -0.035 g/L systematic bias for apolipoprotein (apo) A-I, and a -9% proportional bias for apo B, compared with values in 12-h fasting specimens. Although a physiological hemodilution appears to account for a portion of these bia...

journal_title：Clinical chemistry

authors： Emancipator K

更新日期：1992-03-01 00:00:00

abstract：BACKGROUND:Hyperglycosylated human chorionic gonadotropin (hCG-h) contains larger and more complex carbohydrate chains than regular human chorionic gonadotropin (hCG). hCG-h is thought to be the major form of hCG produced by testicular cancers and it has been suggested to play a key role in tumor invasion, but studies ...

journal_title：Clinical chemistry

authors： Lempiäinen A,Hotakainen K,Blomqvist C,Alfthan H,Stenman UH

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:Whether low plasma 25-hydroxyvitamin D concentrations cause osteoporotic fractures is unclear. We tested the hypothesis that low plasma 25-hydroxyvitamin D concentrations are associated with increased risk of osteoporotic fractures using a Mendelian randomization analysis. METHODS:We genotyped 116 335 rando...

journal_title：Clinical chemistry

authors： Çolak Y,Afzal S,Nordestgaard BG

更新日期：2020-05-01 00:00:00

abstract：BACKGROUND:Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g., RHD or Y-chromosome-specific sequences. To date, the analysis of other fetal geneti...

journal_title：Clinical chemistry

authors： Li Y,Zimmermann B,Rusterholz C,Kang A,Holzgreve W,Hahn S

更新日期：2004-06-01 00:00:00

abstract：BACKGROUND:Protein tyrosine phosphatase 1B (PTPN1) dephosphorylates insulin receptors and attenuates insulin signaling. Polymorphisms in the coding sequence of PTPN1 have been variably associated with type 2 diabetes (T2D). We hypothesized that variations within the PTPN1 promoter might contribute to the development of...

journal_title：Clinical chemistry

authors： Meshkani R,Taghikhani M,Al-Kateb H,Larijani B,Khatami S,Sidiropoulos GK,Hegele RA,Adeli K

更新日期：2007-09-01 00:00:00

abstract：:We measured certain enzyme activities (aldolase, aspartate aminotransferase, creatine kinase, and lactate dehydrogenase) and inflammation markers (alpha 1-antitrypsin, C-reactive protein, fibrinogen, and leukocytes) each day for four days in plasma of patients with severe head injury. The univariate prognostic efficie...

journal_title：Clinical chemistry

authors： Bourguignat A,Albert A,Férard G,Tulasne PA,Kempf I,Métais P

更新日期：1983-11-01 00:00:00

abstract：BACKGROUND:Nandrolone is one of the most abused anabolic steroids, and its use in doping is increasing, as revealed by numerous positive cases during recent years in various sports. Different authors have reported the possible natural production of nandrolone metabolites in humans, and some of these authors argued that...

journal_title：Clinical chemistry

authors： Baume N,Avois L,Schweizer C,Cardis C,Dvorak J,Cauderay M,Mangin P,Saugy M

更新日期：2004-02-01 00:00:00

abstract：:A reaction of urea, o-phthalaldehyde and N-(1-naphthyl)ethylenediamine is described for measurement of urea by manual, continuous-flow, and kinetic methods. The continuous-flow system requires 25 mu-l of sample; 40 samples can be analyzed per hour. The kinetic method requires no enzymes, has no lag phase, and has good...

journal_title：Clinical chemistry

authors： Jung D,Biggs H,Erikson J,Ledyard PU

更新日期：1975-07-01 00:00:00

abstract：:With use of a specially designed gradient-generating system, of anion-exchange resins of 10-20 mum particle diameter, and of an orcinol solution in concentrated sulfuric acid (1 g/liter) as detection reagent, a mixture of 16 carbohydrates can be separated in less than 4 h. The limit of detection for most sugars is in ...

journal_title：Clinical chemistry

authors： Voelter W,Bauer H

更新日期：1975-12-01 00:00:00

abstract：:Four double-monoclonal time-resolved immunofluorometric assays (TrIFMAs) have been developed for the specific determination of pregnancy-associated plasma protein A/proeosinophil major basic protein (PAPP-A/ proMBP) complex in first-trimester maternal serum samples. The assays have a functional sensitivity of < 4 mIU/...

journal_title：Clinical chemistry

authors： Qin QP,Christiansen M,Oxvig C,Pettersson K,Sottrup-Jensen L,Koch C,Nørgaard-Pedersen B

更新日期：1997-12-01 00:00:00

abstract：:We measured cholesterol in capillary blood samples from 9683 volunteers over a four-day on-site community screening program, using the "Reflotron" desk-top analyzer (Boehringer-Mannheim Diagnostics, Indianapolis, IN). We also measured cholesterol in venous blood samples from 3% of those screened (a) with the Reflotron...

journal_title：Clinical chemistry

authors： Bachorik PS,Rock R,Cloey T,Treciak E,Becker D,Sigmund W

更新日期：1990-02-01 00:00:00

abstract：BACKGROUND:Aberrant DNA hypermethylation of CpG islands occurs frequently throughout the genome in human colorectal cancer (CRC). A genome-wide DNA hypermethylation analysis technique using circulating cell-free DNA (cfDNA) is attractive for the noninvasive early detection of CRC and discrimination between CRC and othe...

journal_title：Clinical chemistry

authors： Li J,Zhou X,Liu X,Ren J,Wang J,Wang W,Zheng Y,Shi X,Sun T,Li Z,Kang A,Tang F,Wen L,Fu W

更新日期：2019-07-01 00:00:00

abstract：:Hemoglobinometry according to the International Committee of Standardization in Hematology (ICSH) suffers from imprecision related to high sample dilution and from potential errors owing to sample turbidity. We have evaluated a new instrument, "HemoCue," that measures hemoglobin at two wavelengths as azide methemoglob...

journal_title：Clinical chemistry

authors： von Schenck H,Falkensson M,Lundberg B

更新日期：1986-03-01 00:00:00

abstract：:We describe a new and specific method for measurement of lecithin palmitic acid in amniotic fluid. Dipalmitoyl lecithin, the major alveolar surfactant, has previously been estimated by measuring the lecithin-sphingomyelin ratio, total lecithin, total phospholipid phosphorus, and (or) total palmitic acid. Our method is...

journal_title：Clinical chemistry

authors： Ip MP,Draisey TF,Thibert RJ,Gagneja GL,Jasey GM

更新日期：1977-01-01 00:00:00

abstract：:Serial measurements of the bone and fetal intestinal isoenzymes of alkaline phosphatase (EC 3.1.3.1) in the plasma of 43 term and 43 preterm infants, from birth to six weeks later, indicate that the bone isoenzyme gradually increases over this period in both preterm and term infants fed with unsupplemented commercial ...

journal_title：Clinical chemistry

authors： Crofton PM,Hume R

更新日期：1987-10-01 00:00:00

abstract：BACKGROUND:Identifying markers of chronic kidney disease (CKD) that occur early in the disease process and are specific to loss of kidney function rather than other underlying causes of disease may allow earlier, more accurate identification of patients who will develop CKD. We therefore sought to identify diagnostic b...

journal_title：Clinical chemistry

authors： Mohammadi-Shemirani P,Sjaarda J,Gerstein HC,Treleaven DJ,Walsh M,Mann JF,McQueen MJ,Hess S,Paré G

更新日期：2019-03-01 00:00:00

abstract：:I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum. The antigen (neocarzinostatin), labeled with fluorescein isothiocyanate, was allowed to interact with its antibody in a cuvet, in the instrument, yielding an increas...

journal_title：Clinical chemistry

authors： Maeda H

更新日期：1978-12-01 00:00:00

abstract：:In this manual fluorometric method, blood samples are used that have been impregnated on the filter paper, a convenient collection technique that is widely used to screen newborns for phenylketonuria. The modified procedure, based on the method of McCaman and Robins [J. Lab Clin. Med. 59, 885 (1962)], includes elution...

journal_title：Clinical chemistry

authors： Wu JT,Wu LH,Ziter FA,Ash KO

更新日期：1979-03-01 00:00:00

abstract：BACKGROUND:The bleeding time (BT) test predicts a higher bleeding complication rate in populations at risk for inherited or acquired platelet dysfunction, but it is of limited assistance in evaluating individual patients. There are no reports of clinical outcomes after discontinuation of the BT test. METHODS:Interview...

journal_title：Clinical chemistry

authors： Lehman CM,Blaylock RC,Alexander DP,Rodgers GM

更新日期：2001-01-01 00:00:00

abstract：:We isolated myoglobin from sheep heart by homogenizing cardiac muscle in 70%-saturated ammonium sulfate, followed by chromatography on a column containing carboxymethyl(CM)-Sephadex gel. Two major isoforms of myoglobin, designated Mb 7.9 and Mb 8.1, were separated by chromatofocusing and were distinguished by their di...

journal_title：Clinical chemistry

authors： Wu JT,Pieper RK,Wu LH,Peters JL

更新日期：1989-05-01 00:00:00

abstract：:The Dutch Foundation for Quality Assessment in Clinical Chemistry (SKZL) is the professional organization that conducts external quality-assessment schemes in The Netherlands. However, such schemes in fact assess the performance of the internal quality-control systems of the participating laboratories. In this paper w...

journal_title：Clinical chemistry

authors： Steigstra H,Jansen RT,Baadenhuijsen H

更新日期：1991-07-01 00:00:00

abstract：:We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared b...

journal_title：Clinical chemistry

authors： Thomson S,Wallace AM,Cook B

更新日期：1989-08-01 00:00:00

abstract：:Clottable fibrinogen in human plasma was determined by measuring spectrophotometrically the increase in turbidity with time due to the fibrinogen-fibrin conversion with thrombin. From the maximal absorbance, Amax, at 450 nm obtained 2 min or less after thrombin as added to plasma, we estimated the fibrinogen concentra...

journal_title：Clinical chemistry

authors： Inada Y,Okamoto H,Kanai S,Tamaura Y

更新日期：1978-02-01 00:00:00