Do enzymatic analyses of serum triglycerides really need blanking for free glycerol?

Abstract:

:Distributions of concentrations of free glycerol in clinical plasma obtained for triglyceride assay were compiled to determine the frequency with which increased concentrations of free glycerol posed a potential problem for clinical interpretation of triglyceride results. Clinical histories were studied in patients with increased concentrations of free glycerol to ascertain possible reasons for the increase and to assess the relative clinical importance of glycerol-blank-corrected triglyceride results. Significant increases in free glycerol were very uncommon, usually occurring in patients receiving glycerol-containing hyperalimentation fluids, those receiving heparin (which causes both in vivo and in vitro increases in free glycerol), or those critically ill. Free glycerol was never increased significantly in a large outpatient population. Monitoring lipid metabolism in critically ill patients, or measuring true triglyceride concentrations in patients receiving glycerol-containing fluids, may represent rare exceptions for which glycerol-blank correction is necessary for accurate clinical diagnosis and management. We conclude that there is insufficient justification for the routine expenditure of extra time and reagents to correct most analytical enzymatic triglyceride methods for free glycerol.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Jessen RH,Dass CJ,Eckfeldt JH

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

1372-5

issue

7

eissn

0009-9147

issn

1530-8561

journal_volume

36

pub_type

杂志文章
  • Enzyme-linked immunosorbent choriomammotropin assay.

    abstract::We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coeffi...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Dittmar D,Monji N,Cid A,Malkus H,Castro A

    更新日期:1979-02-01 00:00:00

  • Multilayer film analysis for glucose in 1-microL samples of plasma.

    abstract::With the ultramicroanalytical system described here we can measure glucose in 1 microL of plasma or serum. The sample is placed on a dry, multilayer film element (Eastman Kodak), where a colored spot about 3.5 mm in diameter develops. The reflectance of these spots is measured with a reflectance digital matrix photome...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Neeley WE,Zettner A

    更新日期:1983-12-01 00:00:00

  • HPLC measurement of phenylalanine by direct injection of plasma onto an internal-surface reversed-phase silica support.

    abstract::Direct-injection analysis for phenylalanine in plasma by isocratic "high-performance" liquid chromatography (HPLC) is described. For chromatographic separation I use an internal-surface reversed-phase silica support. The method involves ultraviolet detection, requires as little as 10 microL of plasma (routinely 20 mic...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Atherton ND

    更新日期:1989-06-01 00:00:00

  • Evaluation of the IFCC-recommended procedure for serum aspartate aminotransferase as modified for use with the centrifugal analyzer.

    abstract::The IFCC-recommended procedure for aspartate aminotransferase (EC 2.6.1.1) was adapted to the centrifugal analyzer and evaluated during five years. The main hindrance to widespread use of the recommended method is the need for pre-incubation with pyridoxal phosphate. The present method includes a 10-min pre-incubation...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Bruns DE,Savory J,Titheradge AC,Cross RE,Wills MR

    更新日期:1981-01-01 00:00:00

  • Totally automated liquid-chromatographic assay for cortisol and associated glucocorticoids in serum, with use of ASTED sample preparation.

    abstract::We describe estimation of total cortisol in serum by HPLC. Incorporation of a new technique to totally automate sample preparation decreases costs. The benefits of HPLC ensure that interferences from other steroids are avoided. The method can also be used to estimate concentrations of other glucocorticoids in serum. ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Turnell DC,Cooper JD,Green B,Hughes G,Wright DJ

    更新日期:1988-09-01 00:00:00

  • LC-MS/MS Measurement of Parathyroid Hormone-Related Peptide.

    abstract:INTRODUCTION:Parathyroid hormone-related peptide (PTHrP) is involved in activating pathways, allowing tumor cells to form bone metastases. Measurement of PTHrP is used for the diagnosis and clinical management of patients suspected of hypercalcemia of malignancy. We developed an LC-MS/MS method for measuring PTHrP, est...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2015.244012

    authors: Kushnir MM,Rockwood AL,Strathmann FG,Frank EL,Straseski JA,Meikle AW

    更新日期:2016-01-01 00:00:00

  • Combi scheme: new combined internal/external quality-assessment scheme in The Netherlands.

    abstract::The Dutch Foundation for Quality Assessment in Clinical Chemistry (SKZL) is the professional organization that conducts external quality-assessment schemes in The Netherlands. However, such schemes in fact assess the performance of the internal quality-control systems of the participating laboratories. In this paper w...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Steigstra H,Jansen RT,Baadenhuijsen H

    更新日期:1991-07-01 00:00:00

  • Optimized liquid-chromatographic method for fluorometric determination of urinary delta-aminolevulinic acid in workers exposed to lead.

    abstract::We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working li...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Tomokuni K,Ichiba M,Hirai Y,Hasegawa T

    更新日期:1987-09-01 00:00:00

  • Clinical performance of an in-line, ex vivo point-of-care monitor: a multicenter study.

    abstract:BACKGROUND:The management of critically ill infants and neonates includes frequent determination of arterial blood gas, electrolyte, and hematocrit values. An objective of attached point-of-care patient monitoring is to provide clinically relevant data without the adverse consequences associated with serial phlebotomy....

    journal_title:Clinical chemistry

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Billman GF,Hughes AB,Dudell GG,Waldman E,Adcock LM,Hall DM,Orsini EN Jr,Koska AJ,Van Marter LJ,Finer NN,Kulhavy JC,Feld RD,Widness JA

    更新日期:2002-11-01 00:00:00

  • Single-reagent polarization fluoroimmunoassay for amphetamine in urine.

    abstract::This simple polarization fluoroimmunoassay for detection of amphetamine in urine involves the use of a single reagent: suitably diluted antiserum plus fluorescein-labeled amphetamine as tracer. To this, one adds 10 microL of urine, incubates for a few minutes at room temperature, and measures the fluorescence polariza...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Colbert DL,Gallacher G,Mainwaring-Burton RW

    更新日期:1985-07-01 00:00:00

  • Immunoassay of low concentrations of albumin in urine by latex particle counting.

    abstract::We describe here a nonisotopic immunoassay, based on particle-counting technology, for the determination of urinary albumin. The assay takes only 35 min and has been fully automated on the IMPACT (Acade Diagnostic Systems, Brussels, Belgium) machine. The system measures albumin within a linear range between 6.25 and 5...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Cambiaso CL,Collet-Cassart D,Lievens M

    更新日期:1988-02-01 00:00:00

  • Proteomic Discovery and Validation of the Confounding Effect of Heparin Administration on the Analysis of Candidate Cardiovascular Biomarkers.

    abstract:BACKGROUND:Several plasma proteins have been suggested as markers for a variety of cardiovascular conditions but fail to qualify in independent patient cohorts. This may relate to interference of medication on plasma protein concentrations. We used proteomics to identify plasma proteins that changed in concentration wi...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2017.282665

    authors: Beck HC,Jensen LO,Gils C,Ilondo AMM,Frydland M,Hassager C,Møller-Helgestad OK,Møller JE,Rasmussen LM

    更新日期:2018-10-01 00:00:00

  • Thin film biosensor for rapid visual detection of nucleic acid targets.

    abstract:BACKGROUND:We have developed a silicon-based biosensor that generates a visual signal in response to nucleic acid targets. METHODS:In this system, capture oligonucleotide probes are immobilized on the surface of the biosensor. Interaction of the capture probes with a complementary target and a biotinylated detector ol...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Ostroff RM,Hopkins D,Haeberli AB,Baouchi W,Polisky B

    更新日期:1999-09-01 00:00:00

  • Relationship of lipoprotein(a) to variables of coagulation and fibrinolysis in a healthy population.

    abstract::In the Prospective Cardiovascular Münster (PROCAM) study, serum lipoprotein(a) [Lp(a)] and its relationship to pro- and anticoagulatory as well as fibrinolytic indices were determined in a large group of employees: 864 men (m) and 373 women (f), ages 16-65 years. Univariate statistical analysis showed Lp(a) concentrat...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Heinrich J,Sandkamp M,Kokott R,Schulte H,Assmann G

    更新日期:1991-11-01 00:00:00

  • Gas-chromatographic determination of 5-fluorocytosine in human serum.

    abstract::We describe a sensitive and precise gas-chromatographic method, in which cytosine is used as the internal standard, for determination of an antifungal agent, 5-fluorocytosine, in serum. The trimethylsilyl derivative of this drug is well separated from the internal standard and from normal serum constituents. Amphoteri...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Harding SA,Johnson GF,Solomon HM

    更新日期:1976-06-01 00:00:00

  • Preanalytic and analytic sources of variations in C-reactive protein measurement: implications for cardiovascular disease risk assessment.

    abstract:BACKGROUND:C-reactive protein (CRP) is a widely recognized indicator of inflammation and is known to play an important role in atherogenesis. Recent prospective studies have demonstrated that increased CRP concentrations within the reference interval are a strong predictor of myocardial infarction, stroke, sudden cardi...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/49.8.1258

    authors: Ledue TB,Rifai N

    更新日期:2003-08-01 00:00:00

  • Transferability studies for the AACC reference method and the IFCC method for measurement of alkaline phosphatase activity.

    abstract::We present the results of measurements of alkaline phosphatase activity from interlaboratory transferability studies conducted by 12 laboratories in five countries. The variability, as demonstrated by within-day precision (CV less than or equal to 2.6%), between-day precision (CV less than or equal to 3.6%), and betwe...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Tietz NW,Rinker AD,Burtis C,Duncan P,Ervin K,Ewen L,Hørder M,Mathieu M,Petitclerc C,Grisley D

    更新日期:1984-05-01 00:00:00

  • Detection of alkaline phosphatase/immunoglobulin complexes.

    abstract::We report another patient with a circulating alkaline phosphatase/immunoglobulin complex in his blood, and describe a simple method of demonstrating such complexes. On electrophoresis on cellulose acetate, the complex was relatively slow moving and there was no activity in the normal bone/liver isoenzyme region. When ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Buttery JE,Milner CR,Nenadovic P,Pannall PR

    更新日期:1980-10-01 00:00:00

  • Single-reagent polarization fluoroimmunoassay for barbiturates in urine.

    abstract::We describe a rapid polarization fluoroimmunoassay for screening for the presence of barbiturates in urine. The single reagent is prepared by pre-mixing antiserum with a fluorescein-labeled barbiturate derivative as tracer. Use of a mixture of two sheep antisera, raised against different barbiturate immunogens, result...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Colbert DL,Smith DS,Landon J,Sidki AM

    更新日期:1984-11-01 00:00:00

  • Simplified micro-scale procedure for preparing samples for theophylline determination by liquid chromatography.

    abstract::We describe an improved procedure for the preparation of plasma or serum for determination of theophylline by reverse phase high-performance liquid chromatography. Quantitative results are available in less than 30 min from receipt of sample. The chromatogram is complete in 8 to 16 min, which includes the use of an in...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Nelson JW,Cordry AL,Aron CG,Bartell RA

    更新日期:1977-01-01 00:00:00

  • Endothelial nitric oxide synthase gene polymorphisms and risk of coronary artery disease.

    abstract:BACKGROUND:Endothelial nitric oxide synthase (eNOS) could be a candidate gene for coronary artery disease (CAD). This study investigated the relationship of the eNOS Glu(298)-->Asp and T(786)-->C polymorphisms with the presence and severity of CAD in the Italian population. METHODS:We enrolled 415 unrelated individual...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/49.3.389

    authors: Colombo MG,Paradossi U,Andreassi MG,Botto N,Manfredi S,Masetti S,Biagini A,Clerico A

    更新日期:2003-03-01 00:00:00

  • Plasma F2-isoprostanes and coronary artery calcification: the CARDIA Study.

    abstract:BACKGROUND:Oxidation of lipids in lipoproteins and cells may initiate and enhance the early development of cardiovascular disease. METHOD AND RESULTS:We assayed F(2)-isoprostanes, oxidation products of arachidonic acid, by gas chromatography-mass spectrometry in a biracial cohort of 2850 young healthy adult men and wo...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2004.037630

    authors: Gross M,Steffes M,Jacobs DR Jr,Yu X,Lewis L,Lewis CE,Loria CM

    更新日期:2005-01-01 00:00:00

  • Virus-coated layer-by-layer colloids as a multiplex suspension array for the detection and quantification of virus-specific antibodies.

    abstract:BACKGROUND:Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virus-specific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding color-coded particles. METHODS:We used layer-by-layer technology ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2005.065789

    authors: Toellner L,Fischlechner M,Ferko B,Grabherr RM,Donath E

    更新日期:2006-08-01 00:00:00

  • Effect of use of gel-barrier sampling tubes on determination of some antiepileptic drugs in serum.

    abstract::We evaluated the effect on drug concentration of the duration of sample storage in three different tubes for blood collection: SST (Becton Dickinson), AutoSep (Terumo), and Microvette (Sarstedt), all of which contain an inert barrier (e.g., gel) for cleaner separation of serum from coagulum during centrifugation. With...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Bergqvist Y,Eckerbom S,Funding L

    更新日期:1984-03-01 00:00:00

  • Serum erythropoietin concentrations and iron status in patients on chronic hemodialysis.

    abstract::We studied the relationship between serum erythropoietin (EPO) concentration and iron status in 67 patients undergoing chronic hemodialysis. Serum concentrations of EPO were measured by RIA with recombinant human EPO. The geometric mean of the serum EPO concentration was 10.9 int. units/L (mean +/- SD range = 7.8 - 15...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Seguchi C,Shima T,Misaki M,Takarada Y,Okazaki T

    更新日期:1992-02-01 00:00:00

  • Plasma concentrations of gamma-aminobutyric acid (GABA) and mood disorders: a blood test for manic depressive disease?

    abstract::gamma-Aminobutyric acid (GABA), an inhibitory neurotransmitter that serves about one-third of brain neurons, is involved in the development of depression and in the treatment of depression and mania with pharmacological therapy. Brain activity of GABA may be conveniently measured in plasma, and changes in plasma conce...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Petty F

    更新日期:1994-02-01 00:00:00

  • Lactate measured in diluted and undiluted whole blood and plasma: comparison of methods and effect of hematocrit.

    abstract::We evaluated a new analyzer that measures lactate in undiluted whole blood by direct (or undiluted) amperometry [Nova Stat Profile 7 Analyzer (SP7); Nova Biomedical, Waltham, MA] by comparing it with two other analyzers, one for measuring lactate in whole blood by indirect (or diluted) amperometry [Model 2300; Yellow ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Toffaletti J,Hammes ME,Gray R,Lineberry B,Abrams B

    更新日期:1992-12-01 00:00:00

  • Fetal lung maturity, as assessed by gas-liquid chromatographic determination of phospholipid palmitic acid in amniotic fluid.

    abstract::We describe a new and specific method for measurement of lecithin palmitic acid in amniotic fluid. Dipalmitoyl lecithin, the major alveolar surfactant, has previously been estimated by measuring the lecithin-sphingomyelin ratio, total lecithin, total phospholipid phosphorus, and (or) total palmitic acid. Our method is...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Ip MP,Draisey TF,Thibert RJ,Gagneja GL,Jasey GM

    更新日期:1977-01-01 00:00:00

  • Blood loss from laboratory tests.

    abstract:BACKGROUND:Laboratory tests can be an important source of blood loss in hospitals, especially for newborns and patients in intensive care. The aim of this study was to quantify blood loss for laboratory diagnostic tests in a large number of patients in a teaching hospital. METHODS:We estimated blood loss by multiplyin...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/49.10.1651

    authors: Wisser D,van Ackern K,Knoll E,Wisser H,Bertsch T

    更新日期:2003-10-01 00:00:00

  • Semiautomated procedures for evaluation of carbohydrate-deficient transferrin in the diagnosis of alcohol abuse.

    abstract::Carbohydrate-deficient transferrin (CDT) may now be the most valuable biological marker for diagnosis of alcohol abuse. We compared the diagnostic performance of two new CDT tests, Axis %CDT turbidimetric immunoassay (TIA) and Axis %CDT HPLC, against Specialty Laboratories' isoelectric focusing/immunoblotting/laser de...

    journal_title:Clinical chemistry

    pub_type: 临床试验,杂志文章

    doi:

    authors: Bean P,Liegmann K,Løvli T,Westby C,Sundrehagen E

    更新日期:1997-06-01 00:00:00