Abstract:
:We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%. The assay is simple, rapid, and inexpensive. Albumin excretion was 6.2 (SD 4.1) mg/24 h in healthy subjects (n = 40), 14.7 (SD 7.2) mg/24 h in albumin-test-strip-negative Type I diabetics (n = 11), and 19.7 (SD 16.2) mg/24 h in patients with essential hypertension (n = 12).
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Krämer BK,Jesse U,Ress KM,Schmülling RM,Risler Tsubject
Has Abstractpub_date
1987-04-01 00:00:00pages
609-11issue
4eissn
0009-9147issn
1530-8561journal_volume
33pub_type
杂志文章abstract::We describe a process for preparing a cholesterol-rich lipoprotein extract from bovine serum. The material is used to enhance cholestrol concentration up to 3.5 g/liter in a clear control material from human serum. The optical clarity of the enriched serum is only slightly affected by lyophilization and reconstitution...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::At the clinical level, we have seen the emergence of a subspecialty of affective disorders within psychiatry. Mood clinics, depression units, and affective disorders centers are appearing in many academic and clinical settings, where clinical skill and knowledge can be concentrated and new research furthered. By using...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract:BACKGROUND:Band 5 tartrate-resistant acid phosphatase (TrACP; EC 3. 1.3.2) consists of two isoenzymes, bands 5a and 5b, of which band 5b TrACP is considered to be derived from bone. However, no kinetic method for the specific measurement of band 5b TrACP in serum is available. Our aim was to develop a kinetic assay met...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Radiometric, colorimetric, and two immunochemical methods for measuring total iron-binding capacity are compared. We evaluated the procedures on the basis of precision, applicability to a pediatric population, and accuracy as assessed by analytical recovery of purified transferrin. The immunoephelometric assay for tra...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract:BACKGROUND:Adequate detection of designer steroids in the urine of athletes is still a challenge in doping control analysis and requires knowledge of steroid metabolism. In this study we investigated whether uPA(+/+)-SCID mice carrying functional primary human hepatocytes in their liver would provide a suitable alterna...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.119396
更新日期:2009-10-01 00:00:00
abstract::Measurement of colloid osmotic pressure complements measurements of pulmonary artery wedge pressure for assessing the risks of pulmonary edema and constitutes an increasingly important reference for purposes of guiding selection of colloid or crystalloid fluids in patients with acute cardiac disease. We describe a sim...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::Recent evidence indicates that high-density lipoprotein triglyceride (HDL-Tg) may be a predictor of coronary artery disease. We examined three methods for HDL-Tg measurement, comparing results obtained by measurement of Tg in the supernate after heparin-manganese chloride (heparin-Mn) precipitation of EDTA-treated pla...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Laboratories of the Lipid Research Clinics Program (LRC) maintained the accuracy of their measurements of total cholesterol by using seven pooled serum calibrators supplied by the Centers for Disease Control (CDC). Over the 11-year life of the LRC, each calibrator was prepared in succession and a target value was assi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract:BACKGROUND:Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnos...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2011.161968
更新日期:2011-07-01 00:00:00
abstract::To investigate the use of streptavidin-hapten derivatives as potential protein-tracer conjugates for competitive-type immunoassays, we labeled streptavidin with cortisol and compared biotin-binding activity of the conjugates with that of unlabeled streptavidin. In this model system, streptavidin labeled with one to ap...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:The analysis of bodily fluids using SELDI-TOF MS has been reported to identify signatures of spectral peaks that can be used to differentiate patients with a specific disease from normal or control patients. This report is the 2nd of 2 companion articles describing a validation study of a SELDI-TOF MS approa...
journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
doi:10.1373/clinchem.2007.091496
更新日期:2008-01-01 00:00:00
abstract::In this method, leukocytes were isolated from 6 mL of EDTA-blood by density-gradient centrifugation and subsequently incubated with rhodamine isothiocyanate (RITC)-conjugated high-density lipoproteins (HDL). The receptor-bound conjugate particles were determined by fluorescent flow cytometry and compared with 125I-lab...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::Use of an 125I radioimmunoassay involving antiserum coupled to magnetizable cellulose, after prednisolone-21-phosphate interference is removed by dichloromethane extraction at pH 7.4 and endogenous glucocorticoid interference is removed by selective chemical derivatization with Girard's Reagent T [(carboxymethyl)trime...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::We describe the conjoint measurement in urine of the alcoholic catecholamine metabolites 3,4-dihydroxyphenylglycol (DHPG) and 3-methoxy-4-hydroxyphenylglycol (MHPG). The method involves extraction with ethyl acetate and back-extraction into acetic acid (MHPG), followed by adsorption onto alumina (DHPG). The separation...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::A highly sensitive sandwich-type chemiluminescence enzyme immunoassay for plasma endothelin-I (ET-1), involving no extraction steps, has been developed. Two populations of polyclonal antibodies were used in the present study: One is specific to the C-terminus of the endothelin family of peptides; the other, a Fab' fra...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::We tested a new amylase reagent involving chromogenic substrates (Pantrak, Calbiochem-Behring) for precision, dynamic range, and susceptibility to potential interferences. Two p-nitrophenyl-alpha-maltaosides are used as substrates, which amylase cleaves to shorter-chain p-nitrophenyl maltaosides, the latter then yield...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::The method is based on the selective activating capacity of dithiothreitol on creatine kinase isoenzyme MB, after isoenzyme MM is activated by glutathione. Isolated isoenzymes MM and MB of human and canine origin were assayed individually and in mixtures of known activities. When glutathione was present in the assay m...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
abstract::A potential genetic marker for hypertriglyceridemia and coronary disease, an apolipoprotein-CIII gene allele displaying a single nucleotide substitution, was found in 71% of Arabs with primary hypertriglyceridemia. Over 96% of the unrelated adults possessing this allele showed hypertriglyceridemia. The same allele was...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::We report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitop...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::We describe a rapid polarization fluoroimmunoassay for screening for the presence of barbiturates in urine. The single reagent is prepared by pre-mixing antiserum with a fluorescein-labeled barbiturate derivative as tracer. Use of a mixture of two sheep antisera, raised against different barbiturate immunogens, result...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:5α-Reductase 2 deficiency (5ARD) is a known cause of 46,XY disorders of sex development (DSD). Traditionally, the diagnosis relies on dihydrotestosterone (DHT) measurement, but the results are often equivocal, potentially leading to misdiagnosis. We reviewed alternative approaches for diagnosis of 5ARD. MET...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2012.196501
更新日期:2013-05-01 00:00:00
abstract::Haptoglobin is a hemoglobin-binding protein expressed by a genetic polymorphism as three major phenotypes: 1-1, 2-1, and 2-2. Most attention has been paid to determining haptoglobin phenotype as a genetic fingerprint used in forensic medicine. More recently, several functional differences between haptoglobin phenotype...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1996-10-01 00:00:00
abstract:INTRODUCTION:Commercially available SARS-CoV-2 serological assays based on different viral antigens have been approved for the qualitative determination of anti-SARS-CoV-2 antibodies. However, there are limited published data associating the results from commercial assays with neutralizing antibodies. METHODS:67 speci...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1093/clinchem/hvaa211
更新日期:2020-09-07 00:00:00
abstract::We used gas chromatography-mass spectrometry to analyze serum specimens from a patient whose metabolic ketoacidosis resulted from sniffing a toluene-containing substance; we also analyzed a sample of the abused substance itself and a serum specimen from a normal subject. The blood samples from the patient showed abnor...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::We describe a simple method for evaluating thyroxine-binding globulin capacity and concentration from a single 1-cm blood spot on filter-paper used in a screening program for neonatal hypothyroidism. This method permits prompt diagnosis of about 90% of the infants with thyroxine-binding globulin deficiency in our abno...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::The Glucose-Controlled Insulin Infusion System (Biostator) is a modular, computerized, feedback control system for dynamic control of blood glucose concentrations in diabetics. This on-line glucose analyzer for use with whole blood utilizes a novel enzyme (glucose oxidase)-membrane configuration and an electrochemical...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00
abstract:BACKGROUND:A substantial proportion of the variability in the absorption and clearance of cyclosporin A (CsA) after oral administration has been attributed to variability in liver cytochrome P-450 3A4 (CYP3A4) activity and intestinal P-glycoprotein (P-gp) concentration. A polymorphism in the CYP3A4 promoter region, ter...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::Establishing associations between environmental agents and disease presents challenges to both epidemiologists and toxicologists, particularly in cases of complex gene-environment interactions and when there is a long latency between exposure and disease. Biologic markers, physiological signals that reflect exposure, ...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1994-07-01 00:00:00
abstract::Using 123 specimens, we compared the concordance of three different methods for determining glycohemoglobin (GHb): the Diamat (Bio-Rad Laboratories), an automated analyzer measuring HbA1c by cation-exchange chromatography; an assay with the IMx analyzer (Abbott Laboratories), based on boronate affinity binding; and an...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::The clinical value of measuring concentrations of methylmalonic acid in serum (S-MMA) as an aid in the diagnosis of cobalamin deficiency has recently aroused interest. In 58 healthy subjects, ages 40-68 years, we found a 0.95 reference interval of 0.05-0.37 mumol/L (mean 0.21, SD 0.094). In 33 of the subjects, who wer...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00