Abstract:
BACKGROUND:Neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years and is characterized by a wide clinical and biological heterogeneity, from spontaneously regressive forms to cancers with a rapid and fatal progression. MYCN oncogene amplification is considered the most important prognostic factor to evaluate survival and therapeutic choices in these patients. METHODS:Here we present a new assay for rapid and accurate measurement of MYCN amplification, based on real-time quantitative PCR with the TaqMan(TM) reaction. The degree of MYCN amplification was derived from the ratio of the MYCN oncogene and the single-copy reference gene, beta-actin. The absolute abundance of these two genes in tumor sample DNA was obtained by extrapolation on external calibration curves generated with reference DNA. RESULTS:We found a variable degree of MYCN amplification, from 2 to 29, in 26 of 49 (53%) neuroblastomas. These results were well correlated to those obtained with a competitive PCR assay in the same samples (r = 0. 987). MYCN amplification was associated mainly with advanced cancer stages, and the analysis of overall survival confirmed that the measurement of MYCN amplification is a predictor of patient outcome in neuroblastoma. Patients without MYCN amplification had a cumulative survival significantly higher than patients with low (<9; P = 0.02) and high (>/=9; P = 0.03) oncogene amplification. CONCLUSION:The assay is rapid and reproducible and does not require any post-PCR analytical procedure.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Raggi CC,Bagnoni ML,Tonini GP,Maggi M,Vona G,Pinzani P,Mazzocco K,De Bernardi B,Pazzagli M,Orlando Csubject
Has Abstractpub_date
1999-11-01 00:00:00pages
1918-24issue
11eissn
0009-9147issn
1530-8561journal_volume
45pub_type
杂志文章abstract:BACKGROUND:With the gradual reopening of economies and resumption of social life, robust surveillance mechanisms should be implemented to control the ongoing COVID-19 pandemic. Unlike RT-qPCR, SARS-CoV-2 whole genome sequencing (cWGS) has the added advantage of identifying cryptic origins of the virus, and the extent o...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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abstract::The Dutch Foundation for Quality Assessment in Clinical Chemistry (SKZL) is the professional organization that conducts external quality-assessment schemes in The Netherlands. However, such schemes in fact assess the performance of the internal quality-control systems of the participating laboratories. In this paper w...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.040485
更新日期:2005-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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abstract:BACKGROUND:Iron may promote coronary atherosclerotic disease (CAD) by increasing lipid peroxidation. Studies on biochemical or genetic markers of body iron stores as risk factors for CAD have yielded conflicting results. METHODS:We studied 849 individuals with a clear-cut definition of the CAD phenotype, i.e., with (C...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:2002-06-01 00:00:00
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doi:
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doi:
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pub_type: 杂志文章,多中心研究
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1986-11-01 00:00:00
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journal_title:Clinical chemistry
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