Abstract：
:We report the results of a laboratory and clinical evaluation of a commercial kit procedure (Immuno Nuclear Corp.) for measuring the mid-region of the parathyrin molecule. The estimated dose at 50% binding averaged 130 pmol/L, and the minimum detectable concentration was 14 pmol/L. The within-assay CV was less than or equal to 6.6%, the between-assay CV less than or equal to 12.5%. Relative analytical recoveries of various parathyrin fragments averaged 93% (intact, amino acid residues 1-84), 100% (midregion, 44-68), less than 0.01% (N-terminal, 1-34), and less than 0.01% (C-terminal, 69-84). Correlation of results obtained for 59 patients' samples with a radioimmunoassay for midregion/C-terminal parathyrin performed by the Mayo Medical Laboratory yielded the equation INC = 1.16 (Mayo)-1.94 pmol/L (r = 0.971). Clinical evaluation indicates that the INC results for parathyrin correlate with the diagnoses of patients at least as well as the results obtained with the Mayo assay; in some cases, the INC procedure better distinguishes hyperfunction from normal parathyroid function. The INC procedure can be easily performed in hospital laboratories, with a 4-h turnaround time for results.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Haver VM,Rinker AD,Ko BM,Tietz NWsubject
Has Abstractpub_date
1986-11-01 00:00:00pages
2056-9issue
11eissn
0009-9147issn
1530-8561journal_volume
32pub_type
杂志文章abstract：:In galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. We describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. Galactose is oxidized by galactose oxidase to D-galacto-hexadialdos...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:Zinc protoporphyrin is the predominent fluorescent porphyrin accumulating in erythrocytes as a result of chronic lead absorption or iron-deficiency anemia. Although diagnostic concentrations or thresholds for it in erythrocytes have been inferred, normal adult averages have not been established. We quantitatively asse...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：BACKGROUND:Urinary cross-linked N-telopeptide of type I collagen (NTX) is a reliable bone resorption marker in patients with metabolic bone disease. We assessed a clinically available serum NTX assay suitable for anuric patients on hemodialysis (HD). METHODS:Serum concentrations of NTX, C-terminal telopeptide of type ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.051524
更新日期：2005-12-01 00:00:00
abstract：:Using a continuous-flow (Technicon SMAC) analyzer, we measured the concentration of 20 analytes in capillary blood sera from 149 neonates in an intensive-care unit. Included were term and pre-term babies, both groups with and without asphyxia. The values obtained, especially for those enzymes and analytes that are rel...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：BACKGROUND:Simple, reproducible assays are needed for the quantification of sphingolipids, ceramide (Cer), and sphingoid bases. We developed an HPLC method for simultaneous quantification of total plasma concentrations of Cer, glucosylceramide (GlcCer), and ceramide trihexoside (CTH). METHODS:After addition of sphinga...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2006.079012
更新日期：2007-04-01 00:00:00
abstract：:6-thioguanine (6-TGN) and methyl 6-mercaptopurine nucleotides (Me6-MPNs) are the two major metabolites found in erythrocytes after administration of azathioprine. In an attempt to understand the role of these metabolites in the pharmacologic and toxic activity of thiopurines, we have developed a HPLC method for the si...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:To assess the distribution of serum concentrations of the atherogenic lipoprotein(a) [Lp(a)] in an obese population and the possible effects of weight reduction, we determined Lp(a) in 52 white, moderately obese subjects (16 men and 36 women). The subjects were participating in a weight-reduction program of diet, exer...
journal_title：Clinical chemistry
pub_type： 临床试验,杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：BACKGROUND:Lectin-like oxidized LDL receptor 1 (LOX-1) is implicated in atherothrombotic diseases. Activation of LOX-1 in humans can be evaluated by use of the LOX index, obtained by multiplying the circulating concentration of LOX-1 ligands containing apolipoprotein B (LAB) times that of the soluble form of LOX-1 (sLO...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.140707
更新日期：2010-04-01 00:00:00
abstract：:A modified heparin-Sepharose affinity chromatography procedure (Boberg et al., J. Lipid Res. 18:544-547, 1977) was developed to determine two different triglyceride lipase activities in human post-heparin plasma: hepatic triglyceride lipase (I) and lipoprotein lipase (II). With this procedure, lipoproteins were separa...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：BACKGROUND:Paraoxonase may mitigate oxidative damage and thus lower risk of macrovascular disease. METHODS:DNA samples from 2252 participants in the Coronary Artery Risk Development in Young Adults (CARDIA) study were genotyped for the L55M and Q192R polymorphisms of the PON1 (paraoxonase 1) gene, and paraoxonase acti...
journal_title：Clinical chemistry
pub_type： 杂志文章,多中心研究
doi：10.1373/clinchem.2007.099044
更新日期：2008-04-01 00:00:00
abstract：:We describe a simple polarization fluoroimmunoassay for the detection of several commonly abused drugs in urine. The single reagent used, prepared by mixing four different antisera and three fluorescein-labeled derivatives, produces a polarization signal that is the average of the individual signals of the derivatives...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:To produce bispecific antibodies (BiAbs) for enzyme immunoassay (EIA) to replace antibody-enzyme conjugates, we developed a panel of 8-azaguanine/ouabain-resistant anti-urease variant hybridoma cell lines for use in hybridoma-hybridoma fusions. These variants represent mouse immunoglobulin subclasses IgM, IgG1, IgG2a,...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：BACKGROUND:Microalbuminuria (MA) is recognized as an important risk factor for cardiovascular and renal complications in diabetes. We sought to evaluate how screening for MA is conducted and how urine albumin (UA) results are interpreted in primary care internationally. METHODS:General practitioners (GPs) received a c...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.100917
更新日期：2008-10-01 00:00:00
abstract：BACKGROUND:Data regarding the prognostic value of programmed cell death ligand 1 (PD-L1) expression on circulating tumor cells (CTCs) are lacking. However, CTCs could represent an alternative approach to serial biopsies, allowing real-time monitoring of cancer phenotype. METHODS:We evaluated, in a dedicated prospectiv...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1093/clinchem/hvaa121
更新日期：2020-08-01 00:00:00
abstract：:A recently introduced automated nephelometric immunoassay involving shell/core particles for determination of myoglobin (Behringwerke) was evaluated with the BNA Nephelometer. Method precision was good: the intra-assay CV varied between 1.5% and 6.1%; with daily calibration, the interassay CV ranged between 1.5% and 7...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:We evaluated the homogeneous enzyme immunoassay ("EMIT") of serum thyroxine with use of an ABA- 100 Bichromatic Analyzer, modified with an auxiliary dispenser syringe and a dual probe assembly. The modification allows two reagents to be dispensed at one time. The sera must be pre-treated with 0.1 mol/liter NaOH. The p...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：BACKGROUND:Human iron status is influenced by environmental and genetic factors. We hypothesized that the genetic polymorphism of haptoglobin (Hp), a hemoglobin-binding plasma protein, could affect iron status. METHODS:Reference values of serum iron status markers were compared according to Hp phenotypes (Hp 1-1, Hp 2...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Pairs of values for serum triiodothyronine and serum triiodothyronine binding capacity were represented as maps of points for euthyroid populations (including subjects who were pregnant or were receiving estrogens) in the age groups 18-50, 51-64, 65-79, and greater than 80 years (504 subjects) and thyrotoxic populatio...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：BACKGROUND:Plasma concentrations of small dense (sd)-LDL are associated with the prevalence of cardiovascular events. However, the special equipment and long assay times required for sd-LDL measurement have hindered its clinical application. Herein, we report development of a simple homogeneous assay for sd-LDL-cholest...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2010.149559
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:The alteration of the bone microenvironment as a consequence of skeletal metastases is poorly understood. The aim of this study was to search for patterns of bone markers in relation to primary tumor type, bone pain, and number of sites involved in patients with bone metastases. METHODS:We studied 323 patie...
journal_title：Clinical chemistry
pub_type： 临床试验,杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:In the workup of patients with suspected hereditary nonpolyposis colorectal cancer (HNPCC), detection of loss of heterozygosity (LOH) could help pinpoint the mismatch-repair (MMR) gene carrying the germline mutation, but analysis of microsatellite markers has proved unreliable for this purpose. We developed ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.108902
更新日期：2008-11-01 00:00:00
abstract：:Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:The Center for Disease Control (CDC), the New York State Department of Health (NYSDH), the College of American Pathologists, and 23 manufacturers of diagnostic products participated in an interlaboratory study of aspartate aminotransferase (EC 2.6.1.1) methodologies. Six different lyophilized materials were prepared a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-09-01 00:00:00
abstract：:In this method for measuring amino acids in urine, serum, and tissue, the amino acids to be assayed and the internal standard, L-norleucine, are converted to phenylthiohydantoins , isolated by organic solvent extraction, separated by reversed-phase "high-pressure" liquid chromatography, and detected by ultraviolet abs...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:In this paper, we present initial quality-control evaluations for a prototype clinical laboratory, recommendations for clinical and technical data record-keeping, and laboratory protocols designed to minimize operator-dependent analytical error in a multi-user environment. These evaluations and recommendations are bas...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:We describe use of near-infrared reflectance spectroscopy (NIRRS) to estimate the amount of fat in feces, for diagnosis of steatorrhea. After sample homogenization, the spectrum of the fecal homogenate is scanned over the near-infrared region. Assay of 94 samples of feces having a known concentration of fat showed the...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Major discoveries have been made in the past few years on the structure and mode of biosynthesis of serum albumin. The complete amino acid sequence of this protein has been determined, and its covalent structure shown to be a single peptide chain grouped into a series of nine disulfide-bonded loops. These loops appear...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1977-01-01 00:00:00
abstract：:Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:A dissociation-enhanced lanthanide fluoroimmunoassay of serum cortisol based on time-resolved fluorescence is described. The assay is a direct assay, where cortisol immobilized on the wall of a microtiter-strip well competes with cortisol in the sample for the europium-labeled polyclonal antibody. The amount of bound ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
