Abstract：
BACKGROUND:Until recently, clinical laboratories have monitored hemophilia treatment by measuring coagulation factors before/after infusion of human-derived or recombinant factors. Substantial changes are expected in the near future based on new therapeutic approaches that have been or are being developed. CONTENT:Hemophilia treatment includes replacement therapy with human-derived/recombinant factors or treatment with bypassing agents for patients without or with inhibitors, respectively. Accordingly, laboratory methods for monitoring include one-stage clotting or chromogenic assays meant to measure either factor VIII/IX or global coagulation tests to measure the effect of bypassing agents. Recently, modified long-acting coagulation factors have been introduced for which discrepant results may be expected when measurement is performed with one-stage clotting or chromogenic assays. Currently, novel drugs not based on coagulation factors are under development and are being tested in clinical studies. These drugs do require new methods and therefore laboratory evaluation of hemophilia will undergo dramatic changes in the near future. SUMMARY:From the analysis of the current practice and literature, we draw the following conclusions: (a) Thrombin generation or thromboelastometry are the logical candidate assays to monitor bypassing agents. (b) Considerable differences are expected when measuring modified long-acting coagulation factors, depending on whether one-stage or chromogenic assays are used. Although no definitive conclusions can presently be drawn, chromogenic assays are probably more suitable than one-stage clotting. (c) Novel drugs not based on coagulation factors such as emicizumab, fitusiran, or concizumab that are entering the market do require alternative methods that are not yet well established.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Tripodi A,Chantarangkul V,Novembrino C,Peyvandi Fdoi
10.1373/clinchem.2017.284356subject
Has Abstractpub_date
2019-02-01 00:00:00pages
254-262issue
2eissn
0009-9147issn
1530-8561pii
clinchem.2017.284356journal_volume
65pub_type
杂志文章,评审abstract：BACKGROUND:The B-type natriuretic peptides (BNP and N-terminal pro-BNP) are secreted by the heart and, in the case of BNP, serve to maintain circulatory homeostasis through renal and vascular actions and oppose many effects of the renin-angiotensin system. Recent evidence suggests that in patients with severe heart fai...
journal_title：Clinical chemistry
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doi：10.1373/clinchem.2013.210435
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abstract：:A three-parameter model for directly estimating imprecision profiles from replicated immunoassay results is compared with a six-parameter indirect profile model obtained by the usual method of combining error in the raw response measurements with the slope of the standard curve. Direct estimation is likely to be less ...
journal_title：Clinical chemistry
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doi：
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abstract：:We describe optimal conditions for immobilization of two monoclonal antibodies to progesterone for solid-phase assays. Polystyrene surfaces are refined with Protein A to achieve uniform, reproducible, stable, and sterically accessible immobilization of immunoglobulins (IgG). To this end, we optimized the amount of imm...
journal_title：Clinical chemistry
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abstract：BACKGROUND:The analysis of red cell folate (RCF) depends on complete hemolysis of erythrocytes, and it is assumed that complete hemolysis is achieved by 10-fold dilution of whole blood with hypotonic solutions of 10 g/L ascorbic acid/ascorbate. This report challenges this assumption. METHODS:The conventional method of...
journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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doi：
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doi：10.1373/clinchem.2005.051524
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journal_title：Clinical chemistry
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doi：10.1373/clinchem.2005.062414
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1993-08-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1985-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
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doi：
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
更新日期：1979-07-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1979-11-01 00:00:00
