Single assay for amino-terminal fragments of cardiac A- and B-type natriuretic peptides.

abstract：BACKGROUND:The gene that causes most cases of hereditary hemochromatosis is designated HFE. Individuals with mutations in the HFE gene may have increased serum iron, transferrin saturation, and ferritin concentrations relative to individuals with the wild-type genotype. METHODS:We generated reference centiles for perc...

journal_title：Clinical chemistry

authors： Koziol JA,Ho NJ,Felitti VJ,Beutler E

更新日期：2001-10-01 00:00:00

abstract：BACKGROUND:Paraoxonase may protect lipoproteins and cell membranes from peroxidation, and alterations in the activity of this enzyme have been associated with some chronic diseases. Serum paraoxonase appears to be mainly under genetic control, but some studies suggest that environmental factors may also modulate its ac...

journal_title：Clinical chemistry

authors： Ferré N,Camps J,Fernández-Ballart J,Arija V,Murphy MM,Ceruelo S,Biarnés E,Vilella E,Tous M,Joven J

更新日期：2003-09-01 00:00:00

abstract：:Four routine assays commonly used for monitoring plasma methotrexate (MTX) during high-dose therapy were validated by HPLC as the comparison method. MTX and its main metabolite, 7-hydroxymethotrexate (7-OHMTX), were analyzed by HPLC with postcolumn derivatization and fluorometric detection. About 200 clinical plasma s...

journal_title：Clinical chemistry

authors： Albertioni F,Rask C,Eksborg S,Poulsen JH,Pettersson B,Beck O,Schroeder H,Peterson C

更新日期：1996-01-01 00:00:00

abstract：:We review atomic flame spectrometry of metals based on the selective laser-enhancement of the rate of atomic ionization. A discussion of basic principles, prior literature, and instrumentation is followed by an overview of analytical figures of merit for the technique, and a look at its present status and areas for fu...

journal_title：Clinical chemistry

authors： Travis JC,Turk GC,DeVoe JR

更新日期：1983-09-01 00:00:00

abstract：BACKGROUND:Identifying novel risk markers in cardiovascular patients remains a research priority. Longer follow-up generally is considered favorable in such studies, but associations of interest may become attenuated with increasing follow-up. This issue has not been adequately addressed in the context of patient cohor...

journal_title：Clinical chemistry

authors： Breitling LP,Mons U,Hahmann H,Koenig W,Rothenbacher D,Brenner H

更新日期：2017-03-01 00:00:00

abstract：:In the diagnosis of chronic (as opposed to acute) liver diseases, combinations of indicators are needed to improve specificity. Alanine aminopeptidase (AAP; microsomal aminopeptidase, EC 3.4.11.2) activity in serum reportedly is a very sensitive indicator of intrahepatic cholestasis and biliary obstruction; it is also...

journal_title：Clinical chemistry

authors： Mueller PW,Phillips DL,Steinberg KK

更新日期：1987-03-01 00:00:00

abstract：BACKGROUND:Extracellular matrix alterations are important elements in the arterial changes seen in diabetes, being associated with increased vascular stiffness and the development of cardiovascular diseases. However, no biomarkers for diabetes-related arterial changes have been defined. METHODS:Mammary artery specimen...

journal_title：Clinical chemistry

authors： Cangemi C,Skov V,Poulsen MK,Funder J,Twal WO,Gall MA,Hjortdal V,Jespersen ML,Kruse TA,Aagard J,Parving HH,Knudsen S,Høilund-Carlsen PF,Rossing P,Henriksen JE,Argraves WS,Rasmussen LM

更新日期：2011-11-01 00:00:00

abstract：BACKGROUND:The bleeding time (BT) test predicts a higher bleeding complication rate in populations at risk for inherited or acquired platelet dysfunction, but it is of limited assistance in evaluating individual patients. There are no reports of clinical outcomes after discontinuation of the BT test. METHODS:Interview...

journal_title：Clinical chemistry

authors： Lehman CM,Blaylock RC,Alexander DP,Rodgers GM

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Aberrant DNA hypermethylation of CpG islands occurs frequently throughout the genome in human colorectal cancer (CRC). A genome-wide DNA hypermethylation analysis technique using circulating cell-free DNA (cfDNA) is attractive for the noninvasive early detection of CRC and discrimination between CRC and othe...

journal_title：Clinical chemistry

authors： Li J,Zhou X,Liu X,Ren J,Wang J,Wang W,Zheng Y,Shi X,Sun T,Li Z,Kang A,Tang F,Wen L,Fu W

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:Plasma concentrations of the peptides midregional proadrenomedullin (MR-proADM), midregional proatrial natriuretic peptide (MR-proANP), and C-terminal endothelin-1 precursor fragment (CT-proET-1) are increased in various cardiovascular conditions. However, there is limited information about the association a...

journal_title：Clinical chemistry

authors： Kollerits B,Sturm G,Lamina C,Hammerer-Lercher A,Rantner B,Stadler M,Ziera T,Struck J,Klein-Weigel P,Fraedrich G,Kronenberg F

更新日期：2013-04-01 00:00:00

abstract：:Physiologic systems have substantial reserves in younger individuals. The process of aging and intercurrent pathologic processes gradually eliminate these reserves. Changes in endocrine systems, including menopause in women, androgen deficiency in men, loss of skeletal mass, decrease in growth hormone serum concentrat...

journal_title：Clinical chemistry

authors： Perry HM 3rd

更新日期：1999-08-01 00:00:00

abstract：:A new, highly sensitive immunoradiometric thyrotropin (TSH) assay involving solid-phase-coupled monoclonal antibodies (Boots-Celltech Sucrosep IRMA-TSH) has been evaluated in a wide variety of patients with thyroidal and nonthyroidal illnesses and the results compared with those obtained by conventional diagnostic TSH...

journal_title：Clinical chemistry

authors： Bassett F,Eastman CJ,Ma G,Maberly GF,Smith HC

更新日期：1986-03-01 00:00:00

abstract：BACKGROUND:There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS:Transferrin was iron-saturated by mixing the...

journal_title：Clinical chemistry

authors： Helander A,Husa A,Jeppsson JO

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:The "unit" for "enzymic activity" (U = 1 micromol/min) was recommended by the International Union of Biochemistry and Molecular Biology (IUB) in 1961 and is widely used in medical laboratory reports. The general trend in metrology, however, is toward global standardization through defining units coherent wit...

journal_title：Clinical chemistry

authors： Dybkaer R

更新日期：2002-03-01 00:00:00

abstract：:Urokinase plasminogen activator (uPA) is a serine protease causally involved in cancer invasion and metastasis. In this study, high concentrations of uPA in primary breast cancers were independently associated with both a shortened disease-free interval and overall survival. For the disease-free interval as endpoint, ...

journal_title：Clinical chemistry

authors： Duffy MJ,Duggan C,Mulcahy HE,McDermott EW,O'Higgins NJ

更新日期：1998-06-01 00:00:00

abstract：:The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique. ...

journal_title：Clinical chemistry

authors： Kudirka PJ,Schroeder RR,Hewitt TE,Toren EC Jr

更新日期：1976-04-01 00:00:00

abstract：:Recombinant DNA techniques are contributing to the understanding of the pathogeneses of genetic, neoplastic, and viral diseases, and are used in the diagnosis of certain genetic and viral diseases. Such techniques will have wider application in the future and will play an increasing role in the clinical laboratory. Th...

journal_title：Clinical chemistry

authors： Sauls CD,Caskey CT

更新日期：1985-06-01 00:00:00

abstract：BACKGROUND:A single nonfunctional chimeric gene with its 5' and 3' ends corresponding to CYP21P and CYP21, respectively, is caused by unequal gene crossover in the CYP21 genes during meiosis. The presence of the chimeric CYP21P/CYP21 molecule can not be detected by conventional PCR methods and therefore may be lost in ...

journal_title：Clinical chemistry

authors： Lee HH,Chang JG,Tsai CH,Tsai FJ,Chao HT,Chung B

更新日期：2000-05-01 00:00:00

abstract：:With use of a specially designed gradient-generating system, of anion-exchange resins of 10-20 mum particle diameter, and of an orcinol solution in concentrated sulfuric acid (1 g/liter) as detection reagent, a mixture of 16 carbohydrates can be separated in less than 4 h. The limit of detection for most sugars is in ...

journal_title：Clinical chemistry

authors： Voelter W,Bauer H

更新日期：1975-12-01 00:00:00

abstract：BACKGROUND:Standardization of hemoglobin (Hb)A(1c) measurements is a process of considerable interest for quality assurance in diabetes management. To contribute to continuous quality improvement and fulfillment of the requirements for reference measurement procedures according to the standards of the International Org...

journal_title：Clinical chemistry

authors： Kaiser P,Akerboom T,Ohlendorf R,Reinauer H

更新日期：2010-05-01 00:00:00

abstract：:We describe the conjoint measurement in urine of the alcoholic catecholamine metabolites 3,4-dihydroxyphenylglycol (DHPG) and 3-methoxy-4-hydroxyphenylglycol (MHPG). The method involves extraction with ethyl acetate and back-extraction into acetic acid (MHPG), followed by adsorption onto alumina (DHPG). The separation...

journal_title：Clinical chemistry

authors： Julien C,Rodriguez C,Sacquet J,Cuisinaud G,Sassard J

更新日期：1988-05-01 00:00:00

abstract：:This simple polarization fluoroimmunoassay for detection of amphetamine in urine involves the use of a single reagent: suitably diluted antiserum plus fluorescein-labeled amphetamine as tracer. To this, one adds 10 microL of urine, incubates for a few minutes at room temperature, and measures the fluorescence polariza...

journal_title：Clinical chemistry

authors： Colbert DL,Gallacher G,Mainwaring-Burton RW

更新日期：1985-07-01 00:00:00

abstract：BACKGROUND:Because reference values for vitamin B(12) concentrations and vitamin B(12)-binding capacities for pregnant women have not been established, the reference values for nonpregnant women are often applied to assess vitamin B(12) status. The aim of the present study was to describe ranges of biochemical indices ...

journal_title：Clinical chemistry

authors： Koebnick C,Heins UA,Dagnelie PC,Wickramasinghe SN,Ratnayaka ID,Hothorn T,Pfahlberg AB,Hoffmann I,Lindemans J,Leitzmann C

更新日期：2002-06-01 00:00:00

abstract：BACKGROUND:Pompe disease (PD) is the first lysosomal storage disorder to be added to the Recommended Uniform Screening Panel for newborn screening. This condition has a broad phenotypic spectrum, ranging from an infantile form (IOPD), with severe morbidity and mortality in infancy, to a late-onset form (LOPD) with vari...

journal_title：Clinical chemistry

authors： Lin N,Huang J,Violante S,Orsini JJ,Caggana M,Hughes EE,Stevens C,DiAntonio L,Chieh Liao H,Hong X,Ghomashchi F,Babu Kumar A,Zhou H,Kornreich R,Wasserstein M,Gelb MH,Yu C

更新日期：2017-04-01 00:00:00

abstract：:We studied the EMIT (Enzyme Multiplied Immunoassay Technique, Syva) procedures for the assay of phenytoin and phenobarbital in serum, adapting them to the miniature Centrifugal Fast Analyzer. For different concentrations of drug, each rate of reaction decreased continuously with time, tending to converge on a single c...

journal_title：Clinical chemistry

authors： Lasky FD,Ahuja KK,Karmen A

更新日期：1977-08-01 00:00:00

abstract：:We describe a new and specific method for measurement of lecithin palmitic acid in amniotic fluid. Dipalmitoyl lecithin, the major alveolar surfactant, has previously been estimated by measuring the lecithin-sphingomyelin ratio, total lecithin, total phospholipid phosphorus, and (or) total palmitic acid. Our method is...

journal_title：Clinical chemistry

authors： Ip MP,Draisey TF,Thibert RJ,Gagneja GL,Jasey GM

更新日期：1977-01-01 00:00:00

abstract：:We describe a rapid method for determining cholesterol sulfate in plasma and erythrocytes. After its single-step isolation by means of anion-exchange chromatography cholesterol sulfate is hydrolyzed, trimethylsilylated, and determined by gas chromatography with flame ionization detection. 5 beta-Cholestan-3 alpha-ol s...

journal_title：Clinical chemistry

authors： Muskiet FA,Jansen G,Wolthers BG,Marinkovic-Ilsen A,van Voorst Vader PC

更新日期：1983-07-01 00:00:00

abstract：:We evaluated plasma selenium concentrations in patients with euthyroid sick syndrome express by low serum triiodothyronine (T3) concentrations. Selenium status in these patients was compared with that found in patients with both untreated and treated hypothyroidism. Selenium concentrations in plasma were significantly...

journal_title：Clinical chemistry

authors： Van Lente F,Daher R

更新日期：1992-09-01 00:00:00

abstract：:An enzymatic, fluorometric method is described for determination of serum urea on silicone-rubber pads. In this method, the reagents are lyophilized on the surface of the pads, NADH on one side and a mixture of urease, glutamate dehydrogenase, and alpha-ketoglutarate on the other. The rate of disappearance of NADH flu...

journal_title：Clinical chemistry

authors： Kuan JC,Lau KY,Guilbault GG

更新日期：1975-01-01 00:00:00

abstract：:We recently proposed a quantitative PCR procedure for the absolute measurement of c-erbB-2 oncogene amplification, based on the simultaneous polymerase chain reaction (PCR) amplification of the target gene and of a competitor DNA molecule acting as internal standard. To increase the number of assayable oncogenes and t...

journal_title：Clinical chemistry

authors： Sestini R,Orlando C,Zentilin L,Lami D,Gelmini S,Pinzani P,Giacca M,Pazzagli M

更新日期：1995-06-01 00:00:00