High-pressure liquid-chromatographic separation of lactate dehydrogenase isoenzymes.

abstract：BACKGROUND:Massively parallel sequencing has recently been used in noninvasive prenatal diagnosis. The current costs of this technology are still relatively expensive, however, and sample throughput is still relatively low when it is used as a molecular diagnostic tool. Rather than nonselectively sequencing the genome,...

journal_title：Clinical chemistry

authors： Liao GJ,Lun FM,Zheng YW,Chan KC,Leung TY,Lau TK,Chiu RW,Lo YM

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:The hepatocyte-derived acute-phase reactant C-reactive protein (CRP) has been the subject of intense research over the last 2 decades for its possible role in the pathogenesis of cardiovascular diseases. This research has spawned interest in the use of the blood concentration of CRP for predicting a first co...

journal_title：Clinical chemistry

authors： Hingorani AD,Shah T,Casas JP,Humphries SE,Talmud PJ

更新日期：2009-02-01 00:00:00

abstract：:The concentration of the conjugated bile acid, cholylglycine, in serum is a sensitive and specific indicator of hepatic function. We describe a convenient, specific, and precise radioimmunoassay for cholylglycine, in which 125I-labeled cholylglycyltyrosine is used as tracer. In addition, a blocking agent in the buffer...

journal_title：Clinical chemistry

authors： Miller P,Weiss S,Cornell M,Dockery J

更新日期：1981-10-01 00:00:00

abstract：:Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detec...

journal_title：Clinical chemistry

authors： Flick JA,Schnaar RL,Perman JA

更新日期：1987-07-01 00:00:00

abstract：:We describe a method for measuring apolipoprotein (apo) C distribution between apo B-containing lipoprotein (apo B-LP) and non-apo B-LP. The procedure requires the precipitation of apo B-LP, the redissolution of the pellet, and the quantification of C peptides in the redissolved pellet. The ratio of apo C in non-apo B...

journal_title：Clinical chemistry

authors： Graziani MS,Zanolla L,Righetti G,Marchetti C,Zanotto G,Lupo A

更新日期：1994-02-01 00:00:00

abstract：:We describe a radioassay for cobalamin (vitamin B12) in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is rele...

journal_title：Clinical chemistry

authors： Houts TM,Carney JA

更新日期：1981-02-01 00:00:00

abstract：:We describe estimation of total cortisol in serum by HPLC. Incorporation of a new technique to totally automate sample preparation decreases costs. The benefits of HPLC ensure that interferences from other steroids are avoided. The method can also be used to estimate concentrations of other glucocorticoids in serum. ...

journal_title：Clinical chemistry

authors： Turnell DC,Cooper JD,Green B,Hughes G,Wright DJ

更新日期：1988-09-01 00:00:00

abstract：BACKGROUND:The susceptibility of plasma lipids to oxidation is thought to be a factor contributing to atherogenic risk. Various groups have studied the in vitro oxidizability of isolated LDL and examined the effects of conventional antioxidants. The drawbacks associated with the isolation of LDL for evaluation of in vi...

journal_title：Clinical chemistry

authors： Atkin MA,Gasper A,Ullegaddi R,Powers HJ

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND:Spurious increases in serum troponins, especially troponin T, have been reported in patients with and without acute myocardial syndromes. METHODS:We studied 78 autopsied patients without clinical myocardial infarction (MI) and correlated histologic cardiac findings with antemortem serum creatine kinase (CK)...

journal_title：Clinical chemistry

authors： Ooi DS,Isotalo PA,Veinot JP

更新日期：2000-03-01 00:00:00

abstract：BACKGROUND:Vascular age is an emerging health indicator and predictor of end-organ damage to the heart, brain, and kidney. Although there have been many review publications concerning risk factors for vascular aging, most include cross-sectional epidemiological studies, limiting inferences about temporality. There is a...

journal_title：Clinical chemistry

authors： Kucharska-Newton AM,Stoner L,Meyer ML

更新日期：2019-01-01 00:00:00

abstract：:We describe a ratio gas-chromatographic method for determination of cortisol production rate by measuring the isotope dilution of urinary cortisol metabolites. The method was calibrated by analyzing [3H]tetrahydrocortisol and [3H]tetrahydrocortisone of known specific activities. Results are reasonably well reproducibl...

journal_title：Clinical chemistry

authors： Derks HJ,Muskiet FA,Wolthers BG,Thijssen JH,Drayer NM

更新日期：1977-03-01 00:00:00

abstract：:In this immunoenzymatic assay for human lutropin (hLH) we used bi-specific antibodies (BiAbs) obtained from the fusion of two hybridomas producing antibodies to beta-D-galactosidase and hLh. The BiAb complexed with the enzyme beta-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay ...

journal_title：Clinical chemistry

authors： Bugari G,Poiesi C,Beretta A,Ghielmi S,Albertini A

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Little is known about lipoprotein(a) [Lp(a)] as a predictor of vascular events among ethnic Chinese. We prospectively investigated the association of Lp(a) with cardiovascular disease and all-cause death in a community-based cohort. METHODS:We conducted a community-based prospective cohort study of 3484 par...

journal_title：Clinical chemistry

authors： Chien KL,Hsu HC,Su TC,Sung FC,Chen MF,Lee YT

更新日期：2008-02-01 00:00:00

abstract：:We describe a rapid method for determining cholesterol sulfate in plasma and erythrocytes. After its single-step isolation by means of anion-exchange chromatography cholesterol sulfate is hydrolyzed, trimethylsilylated, and determined by gas chromatography with flame ionization detection. 5 beta-Cholestan-3 alpha-ol s...

journal_title：Clinical chemistry

authors： Muskiet FA,Jansen G,Wolthers BG,Marinkovic-Ilsen A,van Voorst Vader PC

更新日期：1983-07-01 00:00:00

abstract：:Numerous papers have been written to show which combinations of Shewhart-type quality-control charts are optimal for detecting systematic shifts in the mean response of a process, increases in the random error of a process, and linear drift effects in the mean response across the assay batch. One paper by Westgard et ...

journal_title：Clinical chemistry

authors： Bishop J,Nix AB

更新日期：1993-08-01 00:00:00

abstract：:In routine analysis for immunoglobulin light chains in pediatric diagnostics, the age-related reference intervals for serum kappa (kappa) and lambda (lambda) light chains were evaluated in 1543 healthy subjects (newborns to age 16 years, including 168 premature infants). Light-chain analysis was performed by rate neph...

journal_title：Clinical chemistry

authors： Herkner KR,Salzer H,Böck A,Mühl A,Tsaka T,Steger H,Pollak A,Schatten C,Popp W,Huber-Spitzy V

更新日期：1992-04-01 00:00:00

abstract：:A patient with the hyperviscosity syndrome exhibited very high concentrations of intermediate to small circulating immune complexes (CIC), involving 40-50% of the IgG present, with IgG rheumatoid factor activity. We demonstrate that precipitation of CIC by polyethylene glycol in the reaction mixture caused interferenc...

journal_title：Clinical chemistry

authors： Levinson SS,Perry M,Goldman J,Nathan LE

更新日期：1988-04-01 00:00:00

abstract：:An oligonucleotide "melting" procedure was developed whereby we can monitor for a sequence heterogeneity in the gene for apolipoprotein E production. Two oligonucleotides were synthesized, one recognizing the common epsilon 3 allele but not the common epsilon 2 allele, the other recognizing the epsilon 2 allele but no...

journal_title：Clinical chemistry

authors： Funke H,Rust S,Assmann G

更新日期：1986-07-01 00:00:00

abstract：BACKGROUND:With the gradual reopening of economies and resumption of social life, robust surveillance mechanisms should be implemented to control the ongoing COVID-19 pandemic. Unlike RT-qPCR, SARS-CoV-2 whole genome sequencing (cWGS) has the added advantage of identifying cryptic origins of the virus, and the extent o...

journal_title：Clinical chemistry

authors： Harilal D,Ramaswamy S,Loney T,Suwaidi HA,Khansaheb H,Alkhaja A,Varghese R,Deesi Z,Nowotny N,Alsheikh-Ali A,Abou Tayoun A

更新日期：2020-11-01 00:00:00

abstract：:Intra-erythrocytic pH, ATP concentrations, and 2,3-diphosphoglycerate relaxation times were studied in living erythrocytes by "high-resolution" 31P NMR spectroscopy to assess homeostasis within the cells. In uremic patients, intra-erythrocytic pH is significantly decreased before hemodialysis, but is corrected equally...

journal_title：Clinical chemistry

authors： Monti JP,Gallice P,Baas M,Murisasco A,Crevat A

更新日期：1987-01-01 00:00:00

abstract：:We assessed the performance of three commercially available polyclonal immunoassays for apparent cyclosporine in 120 whole-blood specimens collected from transplant recipients just before their next dose of cyclosporine (CsA). The assays were (a) Abbott's TDx fluorescent polarization immunoassay for CsA and its metabo...

journal_title：Clinical chemistry

authors： Strassman MJ,Lensmeyer GL,Wiebe DA,Carlson IH

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:We analyzed the low-dose (1 microg) rapid adrenocorticotropic hormone test (LDST) in 17 patients with a normal hypothalamic-pituitary-adrenal axis to determine reference intervals for the LDST on the basis of poststimulation cortisol increments. METHODS:We analyzed test results for 17 patients (14 females a...

journal_title：Clinical chemistry

authors： Doi SA,Lasheen I,Al-Humood K,Al-Shoumer KA

更新日期：2006-04-01 00:00:00

abstract：:To compare six assays of hyaluronan (hyaluronic acid; HYA) in serum, developed in different laboratories, we analyzed 10 samples from each of three groups: healthy persons, patients with primary biliary cirrhosis, and patients with rheumatoid arthritis. All the assays are based on the use of affinity proteins specific...

journal_title：Clinical chemistry

authors： Lindqvist U,Chichibu K,Delpech B,Goldberg RL,Knudson W,Poole AR,Laurent TC

更新日期：1992-01-01 00:00:00

abstract：:We examined the changes in sensitivity and specificity that would occur with alterations in the sample-collection schedule and (or) cutoff criterion for the increase in hydrogen concentration in breath after administration of doses of lactose in the dietary range. In a breath-analysis test to classify individuals as l...

journal_title：Clinical chemistry

authors： Rosado JL,Solomons NW

更新日期：1983-03-01 00:00:00

abstract：BACKGROUND:Several proteolytically derived fragments from the proline-rich region (PRR) of human inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) have been identified by surface-enhanced or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS or MALDI-TOF-MS) as potential diseas...

journal_title：Clinical chemistry

authors： Song J,Patel M,Rosenzweig CN,Chan-Li Y,Sokoll LJ,Fung ET,Choi-Miura NH,Goggins M,Chan DW,Zhang Z

更新日期：2006-06-01 00:00:00

abstract：:We find lag phases exceeding 20 min in measuring creatine kinase activity, by using the kinetic creatin phosphate leads to creatine assay, in sera of some patients with carcinoma metastatic to the liver. Such long lag phases are accompanied by a decreased apparent enzyme activity. These problems are eliminated by addi...

journal_title：Clinical chemistry

authors： Bruns DE,Morgan WS,Davis JE,Ladenson JH

更新日期：1976-11-01 00:00:00

abstract：INTRODUCTION:Parathyroid hormone-related peptide (PTHrP) is involved in activating pathways, allowing tumor cells to form bone metastases. Measurement of PTHrP is used for the diagnosis and clinical management of patients suspected of hypercalcemia of malignancy. We developed an LC-MS/MS method for measuring PTHrP, est...

journal_title：Clinical chemistry

authors： Kushnir MM,Rockwood AL,Strathmann FG,Frank EL,Straseski JA,Meikle AW

更新日期：2016-01-01 00:00:00

abstract：:Six alpha-amylase (EC 3.2.1.1) isoenzymes have been resolved electrophoretically on cellulose acetate membranes in a discontinuous buffer system. The fastest migrating isoenzymes are of salivary origin (S1, S2, S3), the slower ones of pancreatic origin (P1, P2, P3). We determined the amylase isoenzyme distribution in ...

journal_title：Clinical chemistry

authors： Legaz ME,Kenny MA

更新日期：1976-01-01 00:00:00

abstract：BACKGROUND:Fetuin-A inhibits inflammation and has a protective effect against myocardial ischemia. Its deficiency has been found to be associated with cardiovascular death in patients with end-stage renal failure disease. We investigated the association between plasma fetuin-A and clinical outcome after ST-elevation ac...

journal_title：Clinical chemistry

authors： Lim P,Collet JP,Moutereau S,Guigui N,Mitchell-Heggs L,Loric S,Bernard M,Benhamed S,Montalescot G,Randé JL,Guéret P

更新日期：2007-10-01 00:00:00

abstract：:We examined the comparative behavior of subject-specific multivariate and univariate reference regions, using both computer-generated data and serial (semi-annual) measurements of selected analytes in subjects from a large health-maintenance program. Univariate studies under both homeostatic and random-walk time-serie...

journal_title：Clinical chemistry

authors： Harris EK,Yasaka T,Horton MR,Shakarji G

更新日期：1982-03-01 00:00:00