Quantification of fragments of human serum inter-alpha-trypsin inhibitor heavy chain 4 by a surface-enhanced laser desorption/ionization-based immunoassay.

abstract：:Creatine kinase (CK; EC 2.7.3.2) has been used as an indicator of myocardial cellular damage. In this study we used a Krebs-Henseleit (KH) solution to reperfuse isolated rat hearts after 24 h of cold preservation and collected the KH reperfusate for assay of CK to assess cellular damage. We wanted to determine the sta...

journal_title：Clinical chemistry

authors： Dunphy G,Ely D

更新日期：1990-05-01 00:00:00

abstract：:In this immunoenzymatic assay for human lutropin (hLH) we used bi-specific antibodies (BiAbs) obtained from the fusion of two hybridomas producing antibodies to beta-D-galactosidase and hLh. The BiAb complexed with the enzyme beta-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay ...

journal_title：Clinical chemistry

authors： Bugari G,Poiesi C,Beretta A,Ghielmi S,Albertini A

更新日期：1990-01-01 00:00:00

abstract：:The advent of hybridoma technology has provided the immunotechnologist a finely tunable instrument that should permit a marked advance in the immunologic sciences. The ability to choose the precise antibody required and the virtually unlimited availability of easily purified antibodies have already resulted in the sim...

journal_title：Clinical chemistry

authors： David GS,Wang R,Bartholomew R,Sevier ED,Adams TH,Greene HE

更新日期：1981-09-01 00:00:00

abstract：:Noninvasive monitoring of blood/tissue glucose concentrations has been successfully accomplished in individual diabetic subjects by using near-infrared (NIR) spectroscopy coupled with chemometric methods. Three different spectrometer configurations were tested: a) a Fourier-transform infrared spectrometer with an indi...

journal_title：Clinical chemistry

authors： Robinson MR,Eaton RP,Haaland DM,Koepp GW,Thomas EV,Stallard BR,Robinson PL

更新日期：1992-09-01 00:00:00

abstract：BACKGROUND:Formation of deposits of the insoluble amyloid beta-peptide is believed to be causally related with neurodegeneration in Alzheimer disease (AD). The beta-peptide originates from a larger amyloid precursor protein (APP) by the action of proteolytic enzymes. The first proteolytic event leading to amyloid forma...

journal_title：Clinical chemistry

authors： Verheijen JH,Huisman LG,van Lent N,Neumann U,Paganetti P,Hack CE,Bouwman F,Lindeman J,Bollen EL,Hanemaaijer R

更新日期：2006-06-01 00:00:00

abstract：:For normal and above-normal concentrations of angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity in plasma, results of a manual fluorometric method [with hippuryl-histidyl-leucine (HHL), 5 mmol/L, as substrate] correlated well with those of an automated spectrophotometric method [with 3-(2-furylacryloyl)-L-phen...

journal_title：Clinical chemistry

authors： Gorski TP,Campbell DJ

更新日期：1991-08-01 00:00:00

abstract：:Creatine kinase (CK) isoenzymes MM, MB, and BB are located primarily in the cell cytosol, and increased CKMB in plasma is the hallmark of myocardial infarction. However, whether CK is released with reversible ischemic injury remains controversial. Here, we assessed plasma CK activity--cytosolic and mitochondrial CK--i...

journal_title：Clinical chemistry

authors： Ishikawa Y,Saffitz JE,Mealman TL,Grace AM,Roberts R

更新日期：1997-03-01 00:00:00

abstract：BACKGROUND:The optimal situation in external quality assessment (EQA) is to use commutable materials. No previous study has examined the commutability of a whole-blood material for point-of-care (POC) testing. The aim of this study was to determine the commutability of the Norwegian Quality Improvement of Laboratory Ex...

journal_title：Clinical chemistry

authors： Bukve T,Sandberg S,Vie WS,Sølvik U,Christensen NG,Stavelin A

更新日期：2019-06-01 00:00:00

abstract：:A modified fermentation procedure based on one described by Homer Smith in his textbook was used to eliminate glucose interference in the automated detrmination of inulin. The manifold of Fjeldbo and Stamey [J. Lab. Clin. Med. 72, 353 (1968)] was modified to enable us to insert the fermentation procedure without sacri...

journal_title：Clinical chemistry

authors： Torelli JA,Middleton B,Stein RM

更新日期：1977-07-01 00:00:00

abstract：:We describe use of near-infrared reflectance spectroscopy (NIRRS) to estimate the amount of fat in feces, for diagnosis of steatorrhea. After sample homogenization, the spectrum of the fecal homogenate is scanned over the near-infrared region. Assay of 94 samples of feces having a known concentration of fat showed the...

journal_title：Clinical chemistry

authors： Koumantakis G,Radcliff FJ

更新日期：1987-04-01 00:00:00

abstract：:It has been estimated that approximately 37% of the US population judged to be at high risk for developing coronary artery disease (CAD), based on the National Cholesterol Education Program guidelines, have increased plasma lipoprotein(a) [Lp(a)], whereas Lp(a) is increased in only 14% of those judged to be at low ris...

journal_title：Clinical chemistry

authors： Marcovina SM,Koschinsky ML,Albers JJ,Skarlatos S

更新日期：2003-11-01 00:00:00

abstract：:In photometry, where both the product formed and one or several reactants absorb light at the same wavelength, the absorbance of the "blank" of the sample at the end of the reaction may be less than that measured at the beginning of the reaction, because of consumption of reactant(s). The blank correction factor for t...

journal_title：Clinical chemistry

authors： Kroll MH,Elin RJ

更新日期：1985-03-01 00:00:00

abstract：BACKGROUND:The fibroblast growth factor (FGF) and FGF receptor (FGFR) axis plays a critical role in tumorigenesis, but little is known of its influence in ovarian cancer. We sought to determine the association of genetic variants in the FGF pathway with risk, therapeutic response, and survival of patients with ovarian ...

journal_title：Clinical chemistry

authors： Meng QH,Xu E,Hildebrandt MA,Liang D,Lu K,Ye Y,Wagar EA,Wu X

更新日期：2014-01-01 00:00:00

abstract：:Taurine was measured in cerebrospinal fluid by reacting it with fluorescamine to form a fluorescent derivative, followed by separation on a reversed-phase column and fluorometric detection and evaluation. The assay is rapid (17 min) and sensitive to as little as 1 mumol/L. The mean value for 27 cerebrospinal fluid sam...

journal_title：Clinical chemistry

authors： Shihabi ZK,White JP

更新日期：1979-08-01 00:00:00

abstract：:We measured glucocorticoid receptors (GR) in mononuclear leukocytes (MNL) isolated from peripheral blood of 145 apparently healthy volunteers (86 men and 59 women). An age-related decrease in the number of GR was suggested between subjects younger than 20 years and elderly subjects; there was no apparent seasonal vari...

journal_title：Clinical chemistry

authors： Tanaka H,Akama H,Ichikawa Y,Homma M,Oshima H

更新日期：1991-10-01 00:00:00

abstract：BACKGROUND:Noninvasive prenatal testing (NIPT) uses cell-free DNA (cfDNA) as an analyte to detect copy-number alterations in the fetal genome. Because maternal and fetal cfDNA contributions are comingled, changes in the maternal genome can manifest as abnormal NIPT results. Circulating tumor DNA (ctDNA) present in case...

journal_title：Clinical chemistry

authors： Dharajiya NG,Grosu DS,Farkas DH,McCullough RM,Almasri E,Sun Y,Kim SK,Jensen TJ,Saldivar JS,Topol EJ,van den Boom D,Ehrich M

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:Age-adjusted morbidity and mortality rates from coronary heart disease (CHD) are higher in men than in women. Androgens are suspected to be responsible for the male disadvantage. The genomic effect of androgens is mediated by the androgen receptor (AR), which has a polymorphic CAG repeat in exon 1. The numbe...

journal_title：Clinical chemistry

authors： Hersberger M,Muntwyler J,Funke H,Marti-Jaun J,Schulte H,Assmann G,Lüscher TF,von Eckardstein A

更新日期：2005-07-01 00:00:00

abstract：BACKGROUND:direct DNA sequencing is the primary clinical technique for identifying mutations in human disease, but sequencing often does not detect intragenic or whole-gene deletions. Oligonucleotide array-based comparative genomic hybridization (CGH) is currently in clinical use to detect major changes in chromosomal ...

journal_title：Clinical chemistry

authors： Wong LJ,Dimmock D,Geraghty MT,Quan R,Lichter-Konecki U,Wang J,Brundage EK,Scaglia F,Chinault AC

更新日期：2008-07-01 00:00:00

abstract：:Each of 65 laboratories analyzed 10 whole-blood samples for erythrocyte protoporphyrin by one or more of several analytical procedures. These procedures were of two types: (a) extraction of protoporphyrin from the erythrocytes into ethyl acetate/acetic acid, re-extraction into hydrochloric acid, and fluorometric measu...

journal_title：Clinical chemistry

authors： Jackson KW

更新日期：1978-12-01 00:00:00

abstract：BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...

journal_title：Clinical chemistry

authors： Ryu S,Chang Y,Kim DI,Kim WS,Suh BS

更新日期：2007-01-01 00:00:00

abstract：:Lactate dehydrogenase (LD) isoenzymes 1 and 2 in human serum were separated on a column of diethylaminoethyl-Sephadex. Samples layered on mini-columns were eluted with buffered sodium chloride (100, 150, and 200 mmol/liter). Lactate dehydrogenase activity in column effluents was measured by the Wacker method, and thei...

journal_title：Clinical chemistry

authors： Mercer DW

更新日期：1978-03-01 00:00:00

abstract：BACKGROUND:Variability among assays used to measure intact parathyroid hormone (iPTH) is of particular concern because of the routine use of iPTH assay results to guide management of osteodystrophy and calcium metabolism in patients with end-stage renal disease (ESRD). The aim of this study was to determine the extent ...

journal_title：Clinical chemistry

authors： Cantor T,Yang Z,Caraiani N,Ilamathi E

更新日期：2006-09-01 00:00:00

abstract：BACKGROUND:Insulin, growth hormone (GH), and growth factors (insulin-like growth factors [IGFs] and their binding proteins [IGFBPs]) are known to influence fetal growth and also the synthesis/secretion of the recently discovered hormones leptin and ghrelin. METHODS:In 153 delivering mothers and their offspring at birt...

journal_title：Clinical chemistry

authors： Chiesa C,Osborn JF,Haass C,Natale F,Spinelli M,Scapillati E,Spinelli A,Pacifico L

更新日期：2008-03-01 00:00:00

abstract：:We evaluated a newly developed solid-phase immunoassay (EIA) of terminal deoxynucleotidyl transferase (TdT, EC 2.7.7.3) and compared it with the enzymatic assay of TdT involving DNA polymerase. We assessed the precision, performance characteristics, and clinical efficacy of the EIA procedure, using 249 specimens of pe...

journal_title：Clinical chemistry

authors： Fleisher M,Stankievic R,Schwartz D,Schwartz MK

更新日期：1987-02-01 00:00:00

abstract：:The main developments in the "ligand assay" field in which I have been involved are traced. These include the original development of "first generation" competitive assays relying on radiolabeled analyte markers; the development of the first "second generation", noncompetitive (ultrasensitive) methods, which rely on t...

journal_title：Clinical chemistry

authors： Ekins RP

更新日期：1998-09-01 00:00:00

abstract：:We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensit...

journal_title：Clinical chemistry

authors： Worah D,Yeung KK,Ward FE,Carrico RJ

更新日期：1981-05-01 00:00:00

abstract：BACKGROUND:The hepatocyte-derived acute-phase reactant C-reactive protein (CRP) has been the subject of intense research over the last 2 decades for its possible role in the pathogenesis of cardiovascular diseases. This research has spawned interest in the use of the blood concentration of CRP for predicting a first co...

journal_title：Clinical chemistry

authors： Hingorani AD,Shah T,Casas JP,Humphries SE,Talmud PJ

更新日期：2009-02-01 00:00:00

abstract：:We have developed and validated simple, rapid immunoassays to measure concentrations of albumin in urine ranging from 5 to 200 mg/L. We use antiserum to human albumin, raised in sheep, and we separate the antibody-bound and free fractions of albumin by using a second antiserum, produced in donkeys, against the Fc frag...

journal_title：Clinical chemistry

authors： Silver A,Dawnay A,Landon J,Cattell WR

更新日期：1986-07-01 00:00:00

abstract：:We review the following labels used as substitutes for radioisotopes in immunoassay systems: bacteriophages, chemiluminescence precursors, fluorochromes, fluorogens, fluorescence quenchers, enzymes, coenzymes, inhibitors, substrates, various particulates, metal atoms, and stable free radicals. New methods for performi...

journal_title：Clinical chemistry

authors： Schall RF Jr,Tenoso HJ

更新日期：1981-07-01 00:00:00

abstract：BACKGROUND:Standardization of hemoglobin (Hb)A(1c) measurements is a process of considerable interest for quality assurance in diabetes management. To contribute to continuous quality improvement and fulfillment of the requirements for reference measurement procedures according to the standards of the International Org...

journal_title：Clinical chemistry

authors： Kaiser P,Akerboom T,Ohlendorf R,Reinauer H

更新日期：2010-05-01 00:00:00