Abstract:
BACKGROUND:Fetuin-A inhibits inflammation and has a protective effect against myocardial ischemia. Its deficiency has been found to be associated with cardiovascular death in patients with end-stage renal failure disease. We investigated the association between plasma fetuin-A and clinical outcome after ST-elevation acute myocardial infarction (STEMI). METHODS:We measured fetuin-A in 284 consecutive patients with STEMI and correlated these data with the occurrence of death at 6 months (n = 25). We also measured fetuin-A in a control group and chose the 95th percentile as the cutoff to define abnormality. RESULTS:Patient mean (SD) age was 60 (14) years, and creatinine clearance was 83 (31) mL/min; 82% were men. Mean (SD) plasma fetuin-A concentrations at admission [188 (69) mg/L, P = 0.01] and at day 3 [163 (57) mg/L, P <0.0001] were lower in patients than in controls [219 (39) mg/L; 95th percentile 140 mg/L]. Fetuin-A <140 mg/L was observed in 20% of patients at admission vs 40% at day 3 (P <0.001). Fetuin-A concentrations did not correlate with peak cardiac troponin values but did correlate inversely with C-reactive protein (CRP) and NT-pro-brain natriuretic peptide (NT-proBNP). Fetuin-A <140 mg/L at admission (OR = 3.3, P = 0.03) and at day 3 (OR = 6.3, P = 0.002) was an independent correlate of death at 6 months, irrespective of NT-proBNP, CRP, or Controlled Abciximab and Device Investigation to Lower Late Angioplasty Complications (CADILLAC) risk score. Conversely, fetuin-A > or = 140 mg/L was associated with an excellent survival rate [negative predictive value (NPV) = 97% overall], even in high-risk populations with CADILLAC risk score > or = 6 (NPV = 90% in patients). CONCLUSIONS:Fetuin-A is an important predictor of death at 6 months in STEMI patients independent of NT-proBNP, CRP, and CADILLAC risk score.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Lim P,Collet JP,Moutereau S,Guigui N,Mitchell-Heggs L,Loric S,Bernard M,Benhamed S,Montalescot G,Randé JL,Guéret Pdoi
10.1373/clinchem.2006.084947subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1835-40issue
10eissn
0009-9147issn
1530-8561pii
clinchem.2006.084947journal_volume
53pub_type
杂志文章abstract::We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 mul) of whole blood under conditions of pH, pCO2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The met...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-11-01 00:00:00
abstract:BACKGROUND:A unique requirement for the molecular diagnosis of mitochondrial DNA (mtDNA) disorders is the ability to detect heteroplasmic mtDNA mutations and to distinguish them from homoplasmic sequence variations before further testing (e.g., sequencing) is performed. We evaluated the potential utility of temporal te...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Methods for measuring thyroid autoantibodies--to thyroperoxidase (TPOAb), thyroglobulin (TgAb), and thyrotropin receptor (TRAb)--have improved over the last decade, but increasingly, accurate and sensitive methods are needed for identifying patients with autoimmune thyroid diseases and individuals at high risk for ons...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::A plasma protein required for the support of ristocetin-induced platelet aggregation was isolated from antihemophilic factor concentrate and radiolabeled with 125I. A double-antibody radioimmunoassay was developed, with use of specific rabbit anti-VIII related antigen serum and goat anti-rabbit globulin. The assay is ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-09-01 00:00:00
abstract::The basic theory of thyroxin-analog-based radioimmunoassays for free thyroxin has been extended to evaluate definitively the effects arising from residual binding of the tracer analog to serum proteins. Using experimentally determined binding constants and computer simulation techniques, we studied the effects of thyr...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::The human ras gene plays a fundamental role in the transduction of extracellular signals to the nucleus, thereby regulating cell growth and differentiation. Point mutations in the ras gene convert it into a transforming oncogene that has been found in many solid and hematologic malignancies. We describe a rapid and se...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::We describe modifications to two cartridges, those for alkaline phosphatase and creatinine, on an 18-channel SMAC (Sequential Multichannel Analyzer Computerized), which allow all 18 tests to be run with a commonly prediluted sample. This decreases the required sample size and eliminates the problem of fibrin clots in ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::We compared results obtained with the Beckman Creatinine Analyzer (Beckman Instruments, Inc.) to those with the GEMSAEC Centrifugal Analyzer (Electro-Nucleonics, Inc.) and with a manual method for determination of creatinine in serum and urine. Linearity, stability, recovery, and precision were evaluated. Interference...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::We are developing a method for quantifying plasma lipoproteins by proton nuclear magnetic resonance (NMR) spectroscopy that offers advantages over existing clinical methods. We showed that the major lipoproteins have distinct NMR properties sufficient to permit their concentrations to be extracted from a computer line...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::Serum specimens from patients on primidone therapy were assayed by two currently available procedures: a commercially available enzyme immunoassay (EMIT) and a published gas-chromatographic procedure. Both procedures were done with commercially available materials and equipment. Results by the two procedures agreed we...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract::The kidney and its response to the antidiuretic hormone (ADH) are the principal protective mechanisms necessary to maintain a normal plasma tonicity (osmolality). Hence, determination of the response of the ADH-renal axis to an abnormal plasma tonicity is an important step to understanding water homeostasis. Determina...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1994-07-01 00:00:00
abstract::Use of an 125I radioimmunoassay involving antiserum coupled to magnetizable cellulose, after prednisolone-21-phosphate interference is removed by dichloromethane extraction at pH 7.4 and endogenous glucocorticoid interference is removed by selective chemical derivatization with Girard's Reagent T [(carboxymethyl)trime...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract:BACKGROUND:Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. METHODS:We developed a method for measuring serum testosterone (T) and 5alpha-dihydrotestosterone (DHT) simultaneously via liquid-liquid extraction followed by liquid chromatography-tandem ma...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.103846
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:Fecal calprotectin (FC) has been proposed as a marker of inflammatory bowel disease (IBD), but few studies have evaluated its usefulness in patients with chronic diarrhea of various causes. We evaluated the diagnostic accuracy of a FC assay in identifying "organic" causes of chronic diarrhea in consecutive a...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.6.861
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:In clinical and forensic toxicology, general unknown screening is used to detect and identify exogenous compounds. In this study, we aimed to develop a comprehensive general unknown screening method based on liquid chromatography coupled with a hybrid triple-quadrupole linear ion trap mass spectrometer. MET...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.067116
更新日期:2006-09-01 00:00:00
abstract::Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with bromcresol purple but not with bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indist...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:Eph receptors and their ligands, the ephrins, represent a large class of cell-cell communication molecules with well-defined developmental functions. Their role in healthy adult tissues and in human disease is still largely unknown, although diverse roles in carcinogenesis have been postulated. METHODS:We e...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.026849
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Recurrent pregnancy loss, (RPL) affecting 1%-2% of couples, is defined as ≥3 consecutive pregnancy losses before 20-week' gestation. Women with RPL are routinely screened for etiological factors, but routine screening of male partners is not currently recommended. Recently it has been suggested that sperm qu...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2018.289348
更新日期:2019-01-01 00:00:00
abstract::We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filt...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::We have developed an HPLC method for measuring carbamylated hemoglobin (CarHb), based on the quantification of valine hydantoin formed from the released NH2-terminal carbamyl valine residue after acid hydrolysis of hemoglobin. In uremia, CarHb is produced by nonenzymatic post-translational modification of the terminal...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight wit...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::In routine analysis for immunoglobulin light chains in pediatric diagnostics, the age-related reference intervals for serum kappa (kappa) and lambda (lambda) light chains were evaluated in 1543 healthy subjects (newborns to age 16 years, including 168 premature infants). Light-chain analysis was performed by rate neph...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Serum folate, pteroylglutamic acid standards, and N-5-methyltetrahydrofolate standards extract differently during sample preparation for radiometric assay. B/B0 values for each material were determined before and after an alkaline extraction procedure. Serum folate most closely resembled N-5-methyltetrahydrofolate sta...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-04-01 00:00:00
abstract::We assessed the accuracy and precision of seven commercial kits for serum ferritin. The concentration of ferritin as determined by these assays for a liver ferritin reference standard, a human heart ferritin standard, and normal sera correlated highly, but the absolute values varied widely. Use of an international ref...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract:BACKGROUND:Soluble CD146 (sCD146), is an endothelial marker with similar diagnostic power as natriuretic peptides in decompensated heart failure (HF). While natriuretic peptides are released by the failing heart, sCD146 may be released by veins in response to stretch induced by systemic congestion in HF. This study inv...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:10.1373/clinchem.2016.260471
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Published literature was systematically reviewed to determine the diagnostic accuracy of new protein markers of acute coronary syndromes (ACS) in symptomatic outpatients at low risk of ACS and related complications comparable to patients evaluated in emergency department chest pain units. METHODS:Studies we...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2005.052456
更新日期:2005-11-01 00:00:00
abstract::We evaluated the effect on drug concentration of the duration of sample storage in three different tubes for blood collection: SST (Becton Dickinson), AutoSep (Terumo), and Microvette (Sarstedt), all of which contain an inert barrier (e.g., gel) for cleaner separation of serum from coagulum during centrifugation. With...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract:BACKGROUND:There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS:Transferrin was iron-saturated by mixing the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.023341
更新日期:2003-11-01 00:00:00
abstract::A modified pentamer formation assay (PFA) for quantification of tacrolimus and active metabolites after extraction from whole blood is described. The lower limit of detection was 2 microg/L. Intraassay precision (CV) was 5.7-13.7%, and the interassay CV was 6. 1-14.9%. Tacrolimus trough concentrations in 104 whole blo...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00