Abstract:
:We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 mul) of whole blood under conditions of pH, pCO2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The method consists of gradually oxygenating a blood sample by adding H2O2 in the presence of catalase (EC 1.11.1.6), to produce the reaction H2O2 leads to H2O + 1/2 O2. Because the total oxygen content of blood can be derived from the known rate of H202 addition and the pO2 is determined in the liquid phase by an oxygen electrode, the two functions (total O2 content) and (% oxygen saturation) vs. pO2 are simple to calculate. pCO2 and pH are controlled by adding base simultaneously with the gradual oxygenation of blood. The method described thus avoids the direct measurement of oxygen saturation of whole blood.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Rossi-Bernardi L,Luzzana M,Samaja M,Davi M,DaRiva-Ricci D,Minoli J,Seaton B,Berger RLsubject
Has Abstractpub_date
1975-11-01 00:00:00pages
1747-53issue
12eissn
0009-9147issn
1530-8561journal_volume
21pub_type
杂志文章abstract::We describe a new method for quantitatively measuring substances of clinical interest by high-performance liquid affinity chromatography (HPLAC). As a model system we selected analysis for transferrin in human serum with immobilized antibodies in a high-performance liquid chromatographic system. SelectiSpher-10 Activa...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1976-11-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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更新日期:1996-02-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1989-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1982-01-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1988-07-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1987-10-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
更新日期:1986-01-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:2003-11-01 00:00:00
abstract::In this new radioimmunoassay system for determination of amatoxins in urine and plasma, a novel chemical approach is used for antigen and 125I-tracer production, based on a detoxified alpha-amanitin derivative (aldoamanitin). Total assay time, including data processing, is less than 100 min. The lowest detectable conc...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1982-11-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1986-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1976-07-01 00:00:00