Toward absolute methods in clinical chemistry: application of mass fragmentography to high-accuracy analyses.

Abstract:

:We review recently developed reference methods based on mass fragmentography (specific ion monitoring) with use of isotope-labeled internal standard, for determination of cholesterol, triglycerides, urea, glucose, cortisol, progesterone, and testosterone. With an optimal ratio between standard and material to be determined, the relative standard deviation of these methods, based on duplicate determinations, is between 1.3 and 2.7%. The very high specificity of the methods, in combination with the fact that the ratio between labeled and unlabeled molecules is determined with a high degree of accuracy, makes it likely that the most significant errors in the methods are related to errors in pipetting. The possibility is discussed that these methods might be developed into "absolute" or "definitive" methods by subjecting each individual step in the determination to a detailed analysis with respect to possible error. We also discuss some of the consequences of the different reference methods on the choice of routine methods.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Björkhem I,Blomstrand R,Lantto O,Svensson L,Ohman G

subject

Has Abstract

pub_date

1976-11-01 00:00:00

pages

1789-801

issue

11

eissn

0009-9147

issn

1530-8561

journal_volume

22

pub_type

杂志文章
  • Copy number and expression alterations of miRNAs in the ovarian cancer cell line OVCAR-3: impact on kallikrein 6 protein expression.

    abstract:BACKGROUND:Kallikrein-related peptidase 6 (KLK6), a member of the serine protease family of kallikrein (KLK) genes, is dysregulated in ovarian carcinomas (OCa) and its overexpression is associated with poor prognosis. Regulation of its expression is poorly understood and is likely to be influenced by multiple mechanism...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2012.193060

    authors: Bayani J,Kuzmanov U,Saraon P,Fung WA,Soosaipillai A,Squire JA,Diamandis EP

    更新日期:2013-01-01 00:00:00

  • Highly specific radioimmunoassay for human insulin based on immune exclusion of all insulin precursors.

    abstract::We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed, respectively, against the B10 region, the junction between A chain and C-peptide, and the junction between B chain a...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Deberg M,Houssa P,Frank BH,Sodoyez-Goffaux F,Sodoyez JC

    更新日期:1998-07-01 00:00:00

  • Assessing quality and functionality of DNA from fresh and archival dried blood spots and recommendations for quality control guidelines.

    abstract:BACKGROUND:Dried blood spots (DBS) are a convenient and inexpensive method for biobanking. Although many countries have established population-based DBS biobanks from neonatal screening programs, the quality and usefulness of DNA from DBS have not been extensively assessed. METHODS:We compared 4 common DNA extraction ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2007.087510

    authors: Sjöholm MI,Dillner J,Carlson J

    更新日期:2007-08-01 00:00:00

  • Potentiometry of Na+ in undiluted serum and urine with use of an improved neutral carrier-based solvent polymeric membrane electrode.

    abstract::We present an improved Na+-selective liquid membrane electrode for measurement of Na+ concentrations in both undiluted serum and urine. The values for urinary Na+ obtained with the ion-selective electrode agree well with those obtained with the flame photometer. The correlation gives a standard residual deviation of +...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Anker P,Jenny HB,Wuthier U,Asper R,Ammann D,Simon W

    更新日期:1983-08-01 00:00:00

  • Serum protein profiles by "high-performance" liquid chromatography with detection at multiple wavelengths.

    abstract::We have separated serum proteins in only 15 min by high-performance liquid chromatography. The identification of several peaks in the chromatographic profile was greatly aided by the use of multiple-wavelength detection. We have found a good correlation between retention times and electrophoretic mobilities. Serum sam...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Schlabach TD,Abbott SR

    更新日期:1980-09-01 00:00:00

  • Commutability of a Whole-Blood External Quality Assessment Material for Point-of-Care C-Reactive Protein, Glucose, and Hemoglobin Testing.

    abstract:BACKGROUND:The optimal situation in external quality assessment (EQA) is to use commutable materials. No previous study has examined the commutability of a whole-blood material for point-of-care (POC) testing. The aim of this study was to determine the commutability of the Norwegian Quality Improvement of Laboratory Ex...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2018.300202

    authors: Bukve T,Sandberg S,Vie WS,Sølvik U,Christensen NG,Stavelin A

    更新日期:2019-06-01 00:00:00

  • High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material.

    abstract::We describe specifications for high-purity 4-nitrophenol, which is suitable for spectrophotometric standardization. Such a reference material is needed in clinical enzymology to establish the proper molar absorptivity of 4-nitrophenol under final reaction conditions, particularly for measuring alkaline phosphatase act...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Bowers GN Jr,McComb RB,Christensen RG,Schaffer R

    更新日期:1980-05-01 00:00:00

  • Detection and characterization of blocking-type anti-acetylcholine receptor antibodies in sera from patients with myasthenia gravis.

    abstract::We developed a highly sensitive, convenient assay for measuring blocking-type anti-acetylcholine receptor antibodies, which inhibit the binding of 125I-labeled alpha-bungarotoxin (alpha-BuTx) to the acetylcholine receptor (AChR). This procedure detected inhibitory activities in sera from 76% of patients with myastheni...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Hara H,Hayashi K,Ohta K,Itoh N,Nishitani H,Ohta M

    更新日期:1993-10-01 00:00:00

  • Intravascular monitoring: problems and promise.

    abstract::Intravascular monitoring of arterial blood gases provides the means to detect precipitous change in cardiopulmonary function almost instantaneously, promising major improvement in the ability to manage critically ill patients. Attempts to miniaturize and repackage conventional electrodes for continuous intravascular u...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Halbert SA

    更新日期:1990-08-01 00:00:00

  • Interlaboratory standardization of glycated hemoglobin determinations.

    abstract::As the clinical utility of glycated hemoglobin (gHb) measurement increases, so does the need for standardization of values between different methods and different laboratories. Using three different methods, we examined the feasibility of interlaboratory standardization of gHb measurement. A liquid-chromatographic (HP...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Little RR,England JD,Wiedmeyer HM,McKenzie EM,Mitra R,Erhart PM,Durham JB,Goldstein DE

    更新日期:1986-02-01 00:00:00

  • Improved continuous-flow method for detemination of total serum hexosamines.

    abstract::We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilita...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Sobocinski PZ,Canterbury WJ,Jurgens KH

    更新日期:1976-08-01 00:00:00

  • Steroid characteristics of mineralocorticoid adrenocortical hypertension.

    abstract::Adrenocortical causes of hypertension are established by examining the mineralocorticoid hormones produced in the zona glomerulosa and zona fasciculata. In the zona glomerulosa, aldosterone excess leads to hypertension, hypokalemia, and suppressed plasma renin activity, with increased concentrations of urinary aldoste...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Biglieri EG,Kater CE

    更新日期:1991-10-01 00:00:00

  • Clinical and genetic correlates of growth differentiation factor 15 in the community.

    abstract:BACKGROUND:Growth differentiation factor 15 (GDF15), a stress-responsive cytokine produced in cardiovascular cells under conditions of inflammation and oxidative stress, is emerging as an important prognostic marker in individuals with and without existing cardiovascular disease (CVD). We therefore examined the clinica...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,meta分析

    doi:10.1373/clinchem.2012.190322

    authors: Ho JE,Mahajan A,Chen MH,Larson MG,McCabe EL,Ghorbani A,Cheng S,Johnson AD,Lindgren CM,Kempf T,Lind L,Ingelsson E,Vasan RS,Januzzi J,Wollert KC,Morris AP,Wang TJ

    更新日期:2012-11-01 00:00:00

  • High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.

    abstract:BACKGROUND:High-resolution DNA melting analysis of small amplicons is a simple and inexpensive technique for genotyping. Microfluidics allows precise and rapid control of temperature during melting. METHODS:Using a microfluidic platform for serial PCR and melting analysis, 4 targets containing single nucleotide varian...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2017.276147

    authors: Pryor RJ,Myrick JT,Palais RA,Sundberg SO,Paek JY,Wittwer CT,Knight IT

    更新日期:2017-10-01 00:00:00

  • Hyperamylasemia, specific pancreatic enzymes, and hypoxanthine during recovery from diabetic ketoacidosis.

    abstract::The contribution of the exocrine pancreas to hyperamylasemia in diabetic ketoacidosis was investigated by measuring total amylase, salivary and pancreatic isoamylases, cathodic trypsin-like immunoreactivity, and pancreatic lipase in 12 consecutive patients recovering from diabetic ketoacidosis. Hyperamylasemia was pre...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Møller-Petersen J,Andersen PT,Hjørne N,Ditzel J

    更新日期:1985-12-01 00:00:00

  • Measurement of hemoglobin A1 by liquid chromatography and by agar gel electrophoresis compared.

    abstract::We compare measurement of total fast hemoglobin (HbA1) by "high-performance" liquid chromatography and by electrophoresis on agar gel. Blood samples were obtained from a diverse population (n = 222): offspring of two diabetic parents, diabetic patients with and without retinopathy, diabetic and non-diabetic pregnant w...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Hayes EJ,Gleason RE,Soeldner JS,Wacks M,Blankstein L

    更新日期:1981-03-01 00:00:00

  • Multiplex tetra-primer amplification refractory mutation system PCR to detect 6 common germline mutations of the MUTYH gene associated with polyposis and colorectal cancer.

    abstract:BACKGROUND:We describe a simple tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) for detecting MUTYH mutations, which are associated with colorectal adenomas and colorectal cancer. METHODS:We designed specific T-ARMS-PCR assays for 6 mutations (Y165C, G382D, 1395_7delGGA, Y90X, 1103delC, and R231...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2005.060137

    authors: Piccioli P,Serra M,Gismondi V,Pedemonte S,Loiacono F,Lastraioli S,Bertario L,De Angioletti M,Varesco L,Notaro R

    更新日期:2006-04-01 00:00:00

  • One-step chemiluminescent immunoassay of free thyroxin with acridinium-ester-labeled thyroxin evaluated and compared with a two-step radioimmunoassay.

    abstract::This new one-step chemiluminescent immunoassay of free thyroxin (FT4) involving a thyroxin-immunoglobulin conjugate labeled with acridinium ester (Magic Lite System; Ciba Corning Diagnostics Corp., Medfield, MA) is rapid (one 1-h incubation), requires two calibrators per run, and takes 10 s per sample for the quantifi...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Bounaud JY,Bounaud MP,Begon F

    更新日期:1988-12-01 00:00:00

  • Buprenorphine and norbuprenorphine in hair of pregnant women and their infants after controlled buprenorphine administration.

    abstract:BACKGROUND:Buprenorphine is under investigation as a pharmacotherapeutic agent for treating opioid dependence in pregnant women. We hypothesized that there would be a relationship between the cumulative maternal dose of buprenorphine during pregnancy and the concentration of buprenorphine and norbuprenorphine in matern...

    journal_title:Clinical chemistry

    pub_type: 临床试验,杂志文章

    doi:10.1373/clinchem.2007.091413

    authors: Goodwin RS,Wilkins DG,Averin O,Choo RE,Schroeder JR,Jasinski DR,Johnson RE,Jones HE,Huestis MA

    更新日期:2007-12-01 00:00:00

  • Gas-chromatographic determination of amantadine in human urine.

    abstract::We describe a gas--liquid-chromatographic method for determining the concentration of amantadine hydrochloride in urine with beta-phenylethylamine as internal standard. The urine sample is made alkaline and extracted with 0.5 mL of chloroform. After centrifugation the aqueous layer is aspirated, and an aliquot of the ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Stumph MJ,Noall MW,Knight V

    更新日期:1980-02-01 00:00:00

  • Laboratory diagnosis of Bence Jones proteinuria in a patient with plasma cell leukemia.

    abstract::Kappa Bence Jones proteinuria was found by immunoelectrophoretic techniques in a patient with plasma cell leukemia, who presented with no M-proteins either in serum or urine. However, a significant decrease in normal immunoglobulins was observed. On microscopic examination of kidney sections obtained a autopsy, protei...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Chow C,Spitzer RW

    更新日期:1975-10-01 00:00:00

  • Mycophenolic acid binding to human serum albumin: characterization and relation to pharmacodynamics.

    abstract::Mycophenolate mofetil, the prodrug form of the immunosuppressive agent mycophenolic acid (MPA), is currently in clinical trials evaluating its effectiveness in transplant recipients. In this study, we validated an ultrafiltration system for the reliable measurement of free MPA. Using this technique, we evaluated facto...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Nowak I,Shaw LM

    更新日期:1995-07-01 00:00:00

  • High-performance liquid chromatography of 125I-labeled mouse epidermal growth factor radioiodinated by six different methods.

    abstract::Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Kienhuis CB,Heuvel JJ,Ross HA,Foekens JA,Benraad TJ

    更新日期:1992-05-01 00:00:00

  • Toxicity of free p-cresol: a prospective and cross-sectional analysis.

    abstract:BACKGROUND:Uremic syndrome is the consequence of the retention of solutes usually cleared by the healthy kidneys. p-Cresol can be considered a prototypic protein-bound uremic toxin. It is conceivable, analogous with drugs, that the non-protein-bound fraction of p-cresol exerts toxicity. This aspect had never been evalu...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/49.3.470

    authors: De Smet R,Van Kaer J,Van Vlem B,De Cubber A,Brunet P,Lameire N,Vanholder R

    更新日期:2003-03-01 00:00:00

  • Development and evaluation of a urine protein expert system.

    abstract::Based on the quantitative determination of creatinine, total protein, albumin, alpha 1-microglobulin, IgG, alpha 2-macroglobulin, and N-acetyl-beta, D-glucosaminidase in urine in combination with a test strip screening, the findings of hematuria, leukocyturia, and proteinuria can be assigned to prerenal, renal, or pos...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Ivandić M,Hofmann W,Guder WG

    更新日期:1996-08-01 00:00:00

  • Induced gene transcription: implications for biomarkers.

    abstract::Numerous xenobiotics regulate cellular functions by altering transcription of target genes. Use of sensitive and specific biomarkers based on gene transcript levels may help clarify the shape of the dose-response curve in the low-dose region associated with human exposures to environmental concentrations of chemicals....

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Sewall CH,Bell DA,Clark GC,Tritscher AM,Tully DB,Vanden Heuvel J,Lucier GW

    更新日期:1995-12-01 00:00:00

  • Measurement of glycated protein by a rapid and specific method for absolute quantification of lysine-bound glucose.

    abstract::We modified the liquid-chromatographic assay of Schleicher and Wieland (J Clin Chem Clin Biochem 1981; 19:81-7) for measuring lysine-bound glucose in serum proteins, increasing its performance and practicality. After precipitating serum proteins from 10- to 50-microL samples with ethanol (700 mL/L) and hydrolyzing the...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Drexel H,Klocker H,Patsch JR,Breier C,Braunsteiner H

    更新日期:1987-09-01 00:00:00

  • A direct radioimmunoassay for aldosterone in unextracted serum and plasma.

    abstract::We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactiv...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Lee TP,Tan CH

    更新日期:1981-12-01 00:00:00

  • Anion-exchange chromatographic determination of bromide in serum.

    abstract::In this liquid-chromatographic method for the determination of bromide in human serum, an important and unique feature is pre-analysis ultrafiltration of the sample, which effectively removes proteins. The chromatographic system consists of an anion-exchange column (Whatman Partisil SAX 10-micron particles), an isocra...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Miller ME,Cappon CJ

    更新日期:1984-05-01 00:00:00

  • Iron and total iron-binding capacity in serum of patients receiving iron-dextran: Kodak Ektachem methodologies, spectrophotometry, and atomic-absorption spectrometry compared.

    abstract::We compared the analytical performance of the Kodak Ektachem XR700 assays of iron (Fe) and total iron-binding capacity (TIBC) with that of a conventional ferrozine assay (performed with a Cobas-Bio) and occasionally with that of atomic-absorption spectrometry (AAS). The correlation was modest between Kodak and Cobas-B...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Vercammen M,Goedhuys W,Boeyckens A,De Roy R,Sennesael J,Sevens C,Gorus F

    更新日期:1990-10-01 00:00:00