Abstract:
:Human myocardial creatine kinase isoenzyme MM is present as a single form in tissue, but upon its release into plasma two additional forms, with faster anodal migration, are apparent on polyacrylamide electrophoresis. We designate the three forms as MM3, MM2, and MM1 in increasing order of anodal mobility. When tissue creatine kinase isoenzyme MM (MM3) is incubated with either carboxypeptidase N or carboxypeptidase B it is converted into the two additional forms, MM2 and MM1. The carboxy terminal amino acid of human, canine, and rabbit tissue MM3 was determined to be lysine, a specific substrate for carboxypeptidases N and B. Evidently the mechanism for the production of multiple forms of creatine MM in human plasma is the hydrolysis of a positively charged C-terminal lysine residue from one M subunit (MM2), followed by hydrolysis of the C-terminal lysine from the other subunit (MM1).
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Perryman MB,Knell JD,Roberts Rsubject
Has Abstractpub_date
1984-05-01 00:00:00pages
662-4issue
5eissn
0009-9147issn
1530-8561journal_volume
30pub_type
杂志文章abstract::We studied the association between gamma-glutamyltransferase (GGT) and apolipoproteins A or B in serum of 42 patients with various hepatobiliary diseases. Binding of the enzyme to apolipoprotein A is not related to a clearly defined disease, but appears to be mainly influenced by the ratio of total cholesterol to GGT ...
journal_title:Clinical chemistry
pub_type: 杂志文章
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:1977-01-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1981-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1994-03-01 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2000-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1989-06-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
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doi:10.1373/clinchem.2004.046847
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journal_title:Clinical chemistry
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更新日期:1992-08-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1996-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1997-07-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1979-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
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