Abstract:
BACKGROUND:Gradient gel electrophoresis (GGE) and nuclear magnetic resonance (NMR) spectroscopy are both widely accepted methods for measuring LDL and HDL particle size. However, whether or not GGE- or NMR-measured LDL or HDL particle size predicts coronary heart disease (CHD) risk to a similar extent is currently unknown. METHODS:We used GGE and NMR to measure LDL and HDL particle size in a nested case-control study of 1025 incident cases of CHD and 1915 controls from the EPIC (European Prospective Investigation into Cancer and Nutrition)-Norfolk study. The study sample included apparently healthy men and women age 45-79 years followed for an average of 6 years. RESULTS:Pearson correlation coefficients showed that the overall agreement between NMR and GGE was better for the measurement of HDL size (r = 0.78) than for LDL size (r = 0.47). The odds ratio for future CHD among participants in the bottom tertile of LDL size (smallest LDL particles) was 1.35 (95% CI, 1.12-1.63) for GGE and 1.74 (1.41-2.15) for NMR. For HDL size, these respective odds ratios were 1.41 (1.16-1.72) and 1.85 (1.47-2.32). After adjustment for potential confounders, the relationship between small LDL or HDL particles and CHD was no longer significant, irrespective of the method. CONCLUSIONS:In this prospective population study, we found that the relationships between NMR-measured LDL and HDL sizes and CHD risk were slightly higher than those obtained with GGE.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Arsenault BJ,Lemieux I,Després JP,Wareham NJ,Stroes ES,Kastelein JJ,Khaw KT,Boekholdt SMdoi
10.1373/clinchem.2009.140939subject
Has Abstractpub_date
2010-05-01 00:00:00pages
789-98issue
5eissn
0009-9147issn
1530-8561pii
clinchem.2009.140939journal_volume
56pub_type
杂志文章abstract::We review recently developed reference methods based on mass fragmentography (specific ion monitoring) with use of isotope-labeled internal standard, for determination of cholesterol, triglycerides, urea, glucose, cortisol, progesterone, and testosterone. With an optimal ratio between standard and material to be deter...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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pub_type: 临床试验,杂志文章
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journal_title:Clinical chemistry
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doi:
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1980-04-01 00:00:00
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doi:
更新日期:1987-12-01 00:00:00
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