Two-dimensional gel electrophoresis of human brain proteins. II. Specific proteins and brain subfractions.

abstract：:Using 123 specimens, we compared the concordance of three different methods for determining glycohemoglobin (GHb): the Diamat (Bio-Rad Laboratories), an automated analyzer measuring HbA1c by cation-exchange chromatography; an assay with the IMx analyzer (Abbott Laboratories), based on boronate affinity binding; and an...

journal_title：Clinical chemistry

authors： Turpeinen U,Karjalainen U,Stenman UH

更新日期：1995-02-01 00:00:00

abstract：BACKGROUND:Hypoxic ischemic encephalopathy (HIE) is a major cause of permanent neurological disabilities in full-term newborns. We measured activin A in urine collected immediately after birth in asphyxiated full-term newborns, and assessed the ability of the measurements to predict the occurrence of perinatal encephal...

journal_title：Clinical chemistry

authors： Florio P,Luisi S,Moataza B,Torricelli M,Iman I,Hala M,Hanna A,Petraglia F,Gazzolo D

更新日期：2007-03-01 00:00:00

abstract：:We studied the relationship between serum erythropoietin (EPO) concentration and iron status in 67 patients undergoing chronic hemodialysis. Serum concentrations of EPO were measured by RIA with recombinant human EPO. The geometric mean of the serum EPO concentration was 10.9 int. units/L (mean +/- SD range = 7.8 - 15...

journal_title：Clinical chemistry

authors： Seguchi C,Shima T,Misaki M,Takarada Y,Okazaki T

更新日期：1992-02-01 00:00:00

abstract：:Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are co...

journal_title：Clinical chemistry

authors： Goldberg RN

更新日期：1976-10-01 00:00:00

abstract：BACKGROUND:The fibroblast growth factor (FGF) and FGF receptor (FGFR) axis plays a critical role in tumorigenesis, but little is known of its influence in ovarian cancer. We sought to determine the association of genetic variants in the FGF pathway with risk, therapeutic response, and survival of patients with ovarian ...

journal_title：Clinical chemistry

authors： Meng QH,Xu E,Hildebrandt MA,Liang D,Lu K,Ye Y,Wagar EA,Wu X

更新日期：2014-01-01 00:00:00

abstract：:This assay was developed to measure apolipoprotein (apo) B in blood samples spotted onto filter paper. The long-term aim is to detect young families with the dominantly inherited familial hypercholesterolemia, during current neonatal screening programs. The interassay CV was 5.6%, and apo B concentrations correlated c...

journal_title：Clinical chemistry

authors： Wang XL,Dudman NP,Wilcken DE

更新日期：1989-06-01 00:00:00

abstract：BACKGROUND:Calcitonin (CT) is a sensitive marker for evaluation of medullary thyroid cancer (MTC). However, CT measurement can vary with assay- and nonassay-dependent factors, and procalcitonin (PCT) measurement has been proposed for evaluating questionable increases in CT. METHODS:We tested 2 fully automated CT assay...

journal_title：Clinical chemistry

authors： Kratzsch J,Petzold A,Raue F,Reinhardt W,Bröcker-Preuss M,Görges R,Mann K,Karges W,Morgenthaler N,Luster M,Reiners C,Thiery J,Dralle H,Fuhrer D

更新日期：2011-03-01 00:00:00

abstract：:This relatively simple, rapid method for quantification of homovanillic acid (HVA) and vanillylmandelic acid (VMA) involves solvent extraction and capillary gas chromatography. With use of this extraction method, the overall analytical recovery from an aqueous solution is 92.5% for HVA and 79.2% for VMA. 3,4-Dihydroxy...

journal_title：Clinical chemistry

authors： Tuchman M,Crippin PJ,Krivit W

更新日期：1983-05-01 00:00:00

abstract：BACKGROUND:Methylmalonic acid (MMA) in plasma or serum is widely used for assessment of vitamin B(12) status. However, data are sparse regarding factors, besides renal function, that may influence MMA concentrations. We searched for important determinants of plasma MMA in the general population. METHODS:In 6946 middle...

journal_title：Clinical chemistry

authors： Vogiatzoglou A,Oulhaj A,Smith AD,Nurk E,Drevon CA,Ueland PM,Vollset SE,Tell GS,Refsum H

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:Detection of circulating tumor cells (CTCs) in the peripheral blood is a rapidly developing research field with clear clinical implications for the staging and monitoring of cancer patients. Current CTC assays, including the US Food and Drug Administration-cleared CellSearch® system, typically use markers [e...

journal_title：Clinical chemistry

authors： Pantel K,Denève E,Nocca D,Coffy A,Vendrell JP,Maudelonde T,Riethdorf S,Alix-Panabières C

更新日期：2012-05-01 00:00:00

abstract：:The deleterious, disruptive effects of estrogen mimics on the endocrine system were discovered after the compounds were released into the environment. Their chemical structure does not obviously resemble that of steroid hormones; hence, their estrogenic effects were totally unexpected. In addition to occupational expo...

journal_title：Clinical chemistry

authors： Sonnenschein C,Soto AM,Fernandez MF,Olea N,Olea-Serrano MF,Ruiz-Lopez MD

更新日期：1995-12-01 00:00:00

abstract：:Urokinase plasminogen activator (uPA) is a serine protease causally involved in cancer invasion and metastasis. In this study, high concentrations of uPA in primary breast cancers were independently associated with both a shortened disease-free interval and overall survival. For the disease-free interval as endpoint, ...

journal_title：Clinical chemistry

authors： Duffy MJ,Duggan C,Mulcahy HE,McDermott EW,O'Higgins NJ

更新日期：1998-06-01 00:00:00

abstract：:We studied 25 borderline-hyperparathyroidism patients whose total serum calcium concentration was within normal limits (reference range: 2.25--2.75 mmol/liter) but whose concentrations of serum ionized calcium were above normal (reference range: 1.03--1.23 mmol/liter). Their hyperparathyroidism was histopathologically...

journal_title：Clinical chemistry

authors： Larsson L,Ohman S

更新日期：1978-11-01 00:00:00

abstract：BACKGROUND:Tamoxifen is a standard endocrine therapy for the prevention and treatment of steroid hormone receptor-positive breast cancer. CONTENT:Tamoxifen requires enzymatic activation by cytochrome P450 (CYP) enzymes for the formation of active metabolites 4-hydroxytamoxifen and endoxifen. As compared with the paren...

journal_title：Clinical chemistry

authors： Brauch H,Mürdter TE,Eichelbaum M,Schwab M

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Small RNAs are key players in the regulation of gene expression and differentiation. However, many different classes of small RNAs (sRNAs) have been described with distinct biogenesis pathways and, as a result, with different biochemical properties. To analyze sRNAs by deep sequencing, complementary DNA synt...

journal_title：Clinical chemistry

authors： Meistertzheim M,Fehlmann T,Drews F,Pirritano M,Gasparoni G,Keller A,Simon M

更新日期：2019-12-01 00:00:00

abstract：BACKGROUND:There have been no large-scale analyses of resource utilization comparing the overall costs to treat pediatric patients vs adult patients. Likewise, there have been no studies evaluating the costs of the various components of hospitalization (e.g., accommodation, laboratory, radiology, and drugs) among adult...

journal_title：Clinical chemistry

authors： Young DS,Sachais BS,Jefferies LC

更新日期：2002-01-01 00:00:00

abstract：:Demonstrable quality of laboratory services entails two parts. First, one needs a quality policy statement, identification of user needs, choice of measurement procedures, reference measurement system to provide traceability, control materials, and proficiency testing with materials having reference-measurement-assign...

journal_title：Clinical chemistry

authors： Dybkaer R

更新日期：1994-07-01 00:00:00

abstract：:Erythrocyte protoporphyrin content is increased in several human and veterinary disorders in which heme synthesis is disrupted. The nature of the defect determines whether zinc protoporphyrin, unchelated (free) protoporphyrin, or both are present in the erythrocytes. Extraction of the protoporphyrin into acetone/water...

journal_title：Clinical chemistry

authors： Hart D,Piomelli S

更新日期：1981-02-01 00:00:00

abstract：:We developed a solid-phase two-site immunoenzymometric assay (IEMA) of the estrogen-induced 52-kDa cathepsin D (EC 3.4.23.5) and its processed forms (48-kDa and 34-kDa proteins) in cytosols of breast cancer tissues, using two monoclonal antibodies directed against two different epitopes of these antigens. The first an...

journal_title：Clinical chemistry

authors： Rogier H,Freiss G,Besse MG,Cavalié-Barthez G,Garcia M,Pau B,Rochefort H,Paolucci F

更新日期：1989-01-01 00:00:00

abstract：:The herbicide paraquat (1,1'-dimethyl-4,4'-bipyridylium dichloride; PQ), is a poison known to cause delayed mortality due to lung and kidney injuries. High-resolution proton nuclear magnetic resonance (1H NMR) spectroscopy has been extensively applied in evaluating nephrotoxicity by the characteristic perturbations in...

journal_title：Clinical chemistry

authors： Bairaktari E,Katopodis K,Siamopoulos KC,Tsolas O

更新日期：1998-06-01 00:00:00

abstract：BACKGROUND:Adequate detection of designer steroids in the urine of athletes is still a challenge in doping control analysis and requires knowledge of steroid metabolism. In this study we investigated whether uPA(+/+)-SCID mice carrying functional primary human hepatocytes in their liver would provide a suitable alterna...

journal_title：Clinical chemistry

authors： Lootens L,Meuleman P,Pozo OJ,Van Eenoo P,Leroux-Roels G,Delbeke FT

更新日期：2009-10-01 00:00:00

abstract：:The accuracy of estimates of blood-alcohol concentration based on measurements of breath-alcohol concentration in a randomly selected subject by a random quantitative evidential breath-alcohol analyzer is evaluated with respect to the breath analyzer itself, its calibration, and the biological variables of the subject...

journal_title：Clinical chemistry

authors： Simpson G

更新日期：1987-02-01 00:00:00

abstract：:We describe the interlaboratory testing of a candidate Reference Method (Part I) for uric acid in serum. The method is based on the ultrasound spectrophotometric quantitation of uric acid before and after incubation with uricase. A comprehensive investigation involving 12 laboratories was organized to document the tra...

journal_title：Clinical chemistry

authors： Duncan P,Gochman N,Bayse D,Burnett R,Carey N,Carter R,Fellows WD,Garber C,Kessler G,McComb R,Miller W,Nast P,Ryan W,Schaffer R,Tejeda B,Vanderlinde R,Widdowson G

更新日期：1982-02-01 00:00:00

abstract：:A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without...

journal_title：Clinical chemistry

authors： Ohkubo A,Kamei S,Yamanaka M,Arai F,Kitajima M,Kondo A

更新日期：1981-07-01 00:00:00

abstract：:In this clinically useful enzyme immunoassay of digoxin in serum, we mix sample, beta-galactosidase-labeled digoxin, and anti-digoxin Fab fragments for 30 min at room temperature, then use Sepharose-bound second antibody for phase separation, and measure the unbound enzyme activity directly in the supernate of the equ...

journal_title：Clinical chemistry

authors： Hinds JA,Pincombe CF,Morris H,Duffy P

更新日期：1986-01-01 00:00:00

abstract：BACKGROUND:Little is known about lipoprotein(a) [Lp(a)] as a predictor of vascular events among ethnic Chinese. We prospectively investigated the association of Lp(a) with cardiovascular disease and all-cause death in a community-based cohort. METHODS:We conducted a community-based prospective cohort study of 3484 par...

journal_title：Clinical chemistry

authors： Chien KL,Hsu HC,Su TC,Sung FC,Chen MF,Lee YT

更新日期：2008-02-01 00:00:00

abstract：:We describe an improved procedure for the preparation of plasma or serum for determination of theophylline by reverse phase high-performance liquid chromatography. Quantitative results are available in less than 30 min from receipt of sample. The chromatogram is complete in 8 to 16 min, which includes the use of an in...

journal_title：Clinical chemistry

authors： Nelson JW,Cordry AL,Aron CG,Bartell RA

更新日期：1977-01-01 00:00:00

abstract：:We describe a rapid, sensitive, and specific "high performance" liquid chromatographic analysis for disopyramide and its mono-N-dealkylated metabolite in serum, urine, and saliva. We used a mu-Bondapak CN column and an acetate buffer mobile phase containing methanol. Retention times for the two compounds and the inter...

journal_title：Clinical chemistry

authors： Lima JJ

更新日期：1979-03-01 00:00:00

abstract：BACKGROUND:Because reference values for vitamin B(12) concentrations and vitamin B(12)-binding capacities for pregnant women have not been established, the reference values for nonpregnant women are often applied to assess vitamin B(12) status. The aim of the present study was to describe ranges of biochemical indices ...

journal_title：Clinical chemistry

authors： Koebnick C,Heins UA,Dagnelie PC,Wickramasinghe SN,Ratnayaka ID,Hothorn T,Pfahlberg AB,Hoffmann I,Lindemans J,Leitzmann C

更新日期：2002-06-01 00:00:00

abstract：:A sensitive, specific, and simple method for determining serum or urine arylesterase (EC 3.1.1.2) is described. The enzyme acts on phenyl acetate to release phenol, which produces a stable indophenol dye with 4-aminoantipyrine and potassium ferricyanide. Arylesterase, a thiol enzyme, is reactivated by 2-mercaptoethano...

journal_title：Clinical chemistry

authors： Lorentz K,Flatter B,Augustin E

更新日期：1979-10-01 00:00:00