Plasma glucose concentrations of whole blood, as determined with a multilayer-film analytical element.

Abstract:

:A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without further manipulations. Precise measurement of sample volume spotted on the film is not necessary for good results, nor is knowledge of the hematocrit in the range 10-46%. The minimum concentration of glucose detectable by this method is 100 mg/L. The coefficients of variation, within-run and between-run, were all less than 2.5% for 800-4000 mg/L glucose concentrations. Macromolecules and hydrophobic substances in blood did not interfere. This method seems suitable for emergency use, especially.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Ohkubo A,Kamei S,Yamanaka M,Arai F,Kitajima M,Kondo A

subject

Has Abstract

pub_date

1981-07-01 00:00:00

pages

1287-90

issue

7

eissn

0009-9147

issn

1530-8561

journal_volume

27

pub_type

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