Abstract:
BACKGROUND:TMPRSS2:ERG fusions are promising prostate cancer biomarkers. Because they can occur in multiple forms in a single cancer specimen, we developed a quantitative PCR test that detects both type III and type VI TMPRSS2:ERG fusions. The assay is quantified from a standard curve determined with a plasmid-cloned type III TMPRSS2:ERG fusion target. METHODS:We collected expressed prostatic secretion (EPS) under an institutional review board-approved, blinded, prospective study from 74 patients undergoing transrectal ultrasound-guided biopsy for prostate cancer. We compared the characteristic performance of the test for type III and type VI TMPRSS2:ERG fusions in predicting biopsy outcome and distinguishing between high and low Gleason scores with similar tests for the expression of PCA3 and DNA methylation levels of the APC, RARB, RASSF1, and GSTP1 genes. We used logistic regression to analyze the effects of multiple biomarkers in linear combinations. RESULTS:Each test provided a significant improvement in characteristic performance over baseline digital rectal examination (DRE) plus serum prostate-specific antigen (PSA); however, the test for type III and type VI TMPRSS2:ERG fusions yielded the best performance in predicting biopsy outcome [area under the curve (AUC) 0.823, 95% CI 0.728-0.919, P < 0.001] and Gleason grade >7 (AUC 0.844, 95% CI 0.740-0.948, P < 0.001). CONCLUSIONS:Although each test appears to have diagnostic value, PSA plus DRE plus type III and type VI TMPRSS2:ERG provided the best diagnostic performance in EPS specimens.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Clark JP,Munson KW,Gu JW,Lamparska-Kupsik K,Chan KG,Yoshida JS,Kawachi MH,Crocitto LE,Wilson TG,Feng Z,Smith SSdoi
10.1373/clinchem.2008.108845subject
Has Abstractpub_date
2008-12-01 00:00:00pages
2007-17issue
12eissn
0009-9147issn
1530-8561pii
clinchem.2008.108845journal_volume
54pub_type
杂志文章abstract:BACKGROUND:The new Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation was developed to address the systematic underestimation of the glomerular filtration rate (GFR) by the Modification of Diet in Renal Disease (MDRD) Study equation in patients with a relatively well-preserved kidney function. The per...
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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pub_type: 杂志文章
doi:
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doi:
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doi:
更新日期:1992-08-01 00:00:00
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更新日期:1987-10-01 00:00:00
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