Determination of serum bilirubin concentration during phototherapy of newborns and in vitro: results compared by the direct spectrometric method and the diazo method.

abstract：BACKGROUND:Galectin-3 has emerged as a potential useful novel biomarker for heart failure and cardiovascular disease (CVD). However, it remains unclear whether galectin-3 is associated with recurrent cardiovascular events during long-term follow-up of patients with stable coronary heart disease (CHD) after adjustment f...

journal_title：Clinical chemistry

authors： Jansen H,Koenig W,Jaensch A,Mons U,Breitling LP,Scharnagl H,Stojakovic T,Schunkert H,Brenner H,Rothenbacher D

更新日期：2016-10-01 00:00:00

abstract：:We assessed the performance of three commercially available polyclonal immunoassays for apparent cyclosporine in 120 whole-blood specimens collected from transplant recipients just before their next dose of cyclosporine (CsA). The assays were (a) Abbott's TDx fluorescent polarization immunoassay for CsA and its metabo...

journal_title：Clinical chemistry

authors： Strassman MJ,Lensmeyer GL,Wiebe DA,Carlson IH

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Intravenous and subcutaneous microdialysis was performed to compare the free concentrations and pharmacokinetics of L-3, 4-dihyroxyphenylalanine (L-dopa) in blood and tissue in healthy subjects and in patients with Parkinson disease. METHODS:Nine healthy volunteers and 10 patients with Parkinson disease, st...

journal_title：Clinical chemistry

authors： Dizdar N,Kullman A,Norlander B,Olsson JE,Kagedal B

更新日期：1999-10-01 00:00:00

abstract：BACKGROUND:Cystic fibrosis is a life-threatening genetic disorder that has been associated with mutations in the CFTR [cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)] gene. Hundreds of CFTR mutations have been detected to date. Current CFTR genotyping assays target a s...

journal_title：Clinical chemistry

authors： Abou Tayoun AN,Tunkey CD,Pugh TJ,Ross T,Shah M,Lee CC,Harkins TT,Wells WA,Tafe LJ,Amos CI,Tsongalis GJ

更新日期：2013-10-01 00:00:00

abstract：:The main developments in the "ligand assay" field in which I have been involved are traced. These include the original development of "first generation" competitive assays relying on radiolabeled analyte markers; the development of the first "second generation", noncompetitive (ultrasensitive) methods, which rely on t...

journal_title：Clinical chemistry

authors： Ekins RP

更新日期：1998-09-01 00:00:00

abstract：BACKGROUND:Advances in the biological sciences and technology are providing molecular targets for diagnosing and treating cancer. Current classifications in surgical pathology for staging malignancies are based primarily on anatomic features (e.g., tumor-node-metastasis) and histopathology (e.g., grade). Microarrays to...

journal_title：Clinical chemistry

authors： Bernard PS,Wittwer CT

更新日期：2002-08-01 00:00:00

abstract：:Since 1991, the Ontario Laboratory Proficiency Testing Program has assessed the analytical performance of creatine kinase (CK; EC 2.7.3.2) isoenzyme-2, using fresh human serum supplemented with purified human CK isoenzymes. In Ontario, the 142 laboratories licensed to analyze CK-2 use a variety of methods: electrophor...

journal_title：Clinical chemistry

authors： Henderson AR,Stark JA,McQueen MJ,Patten RL,Krishnan S,Wood DE,Webb S

更新日期：1994-02-01 00:00:00

abstract：:A total of 1357 polypeptides [946 acidic (isoelectric focusing) and 411 basic (nonequilibrium pH-gradient electrophoresis)] from human HeLa cells have been separated and catalogued with use of high-resolution two-dimensional gel electrophoresis. Of these polypeptides, 1266 were detected by labeling cells with [35S]met...

journal_title：Clinical chemistry

authors： Bravo R,Celis JE

更新日期：1982-04-01 00:00:00

abstract：BACKGROUND:Next generation sequencing (NGS)-based assays continue to redefine the field of genetic testing. Owing to the complexity of the data, bioinformatics has become a necessary component in any laboratory implementing a clinical NGS test. CONTENT:The computational components of an NGS-based work flow can be conc...

journal_title：Clinical chemistry

authors： Oliver GR,Hart SN,Klee EW

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...

journal_title：Clinical chemistry

authors： Ryu S,Chang Y,Kim DI,Kim WS,Suh BS

更新日期：2007-01-01 00:00:00

abstract：:Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a DNA sequence for diagnostic purposes. We have combined SDA with fluorescence polarization detection in a closed, homogeneous format. A fluorescently labeled oligodeoxynucleotide detector probe hybridizes to the amplification prod...

journal_title：Clinical chemistry

authors： Walker GT,Nadeau JG,Linn CP,Devlin RF,Dandliker WB

更新日期：1996-01-01 00:00:00

abstract：:In considering factors that might influence measurement of free thyroxin (T4), we evaluated the proportion (%) of T4 that could be stripped from thyroxin-binding globulin (TBG). The percentage of free T4 was measured in serially diluted sera from four normal subjects, four patients with hyperthyroidism or hypothyroidi...

journal_title：Clinical chemistry

authors： Nakagawa T,Matsumura K,Takeda K,Shinoda N,Matsuda A,Matsushita T,Tagami T

更新日期：1990-02-01 00:00:00

abstract：BACKGROUND:Reports of the use of multiplex enzyme assay screening for Pompe disease, Fabry disease, Gaucher disease, Niemann-Pick disease types A and B, and Krabbe disease have engendered interest in the use of this assay in newborn screening. We modified the assay for high-throughput use in screening laboratories. ME...

journal_title：Clinical chemistry

authors： Zhang XK,Elbin CS,Chuang WL,Cooper SK,Marashio CA,Beauregard C,Keutzer JM

更新日期：2008-10-01 00:00:00

abstract：:Cardiac troponin I (cTnI) is a regulatory protein unique to myocardium. We used a cardiospecific 30-min ELISA to measure cTnI in EDTA-plasma samples serially drawn from 28 patients before and after coronary artery bypass grafting (CABG)--26 elective and 2 salvage cases. The cTnI increase in 22 of the elective CABG pat...

journal_title：Clinical chemistry

authors： Mair J,Larue C,Mair P,Balogh D,Calzolari C,Puschendorf B

更新日期：1994-11-01 00:00:00

abstract：:In the present clinical evaluation of Triage immunoassay for detection of drugs of abuse, we examined a possible connection between the laboratory skills of the personnel using it and the quality of analytical results; we also evaluated the degree of concordance between the Triage results and those obtained by an inst...

journal_title：Clinical chemistry

authors： de la Torre R,Domingo-Salvany A,Badia R,Gonzàlez G,McFarlane D,San L,Torrens M

更新日期：1996-09-01 00:00:00

abstract：BACKGROUND:Efforts to reduce the high global prevalence of nutritional anemia require the use of both reliable laboratory assays to distinguish iron deficiency from other causes of anemia and cost-effective methods for collection of blood specimens under field conditions. The suitability of using small plasma samples s...

journal_title：Clinical chemistry

authors： Flowers CH,Cook JD

更新日期：1999-10-01 00:00:00

abstract：:To produce bispecific antibodies (BiAbs) for enzyme immunoassay (EIA) to replace antibody-enzyme conjugates, we developed a panel of 8-azaguanine/ouabain-resistant anti-urease variant hybridoma cell lines for use in hybridoma-hybridoma fusions. These variants represent mouse immunoglobulin subclasses IgM, IgG1, IgG2a,...

journal_title：Clinical chemistry

authors： Takahashi M,Fuller SA

更新日期：1988-09-01 00:00:00

abstract：BACKGROUND:The discovery of cell-free fetal DNA in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis and monitoring. Among the fetal markers that have been described, methylation markers are sex and polymorphism independent. Methylation-sensitive restriction endonucleases are commonly u...

journal_title：Clinical chemistry

authors： Tong YK,Chiu RW,Leung TY,Ding C,Lau TK,Leung TN,Lo YM

更新日期：2007-11-01 00:00:00

abstract：:The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique. ...

journal_title：Clinical chemistry

authors： Kudirka PJ,Schroeder RR,Hewitt TE,Toren EC Jr

更新日期：1976-04-01 00:00:00

abstract：BACKGROUND:The gene that causes most cases of hereditary hemochromatosis is designated HFE. Individuals with mutations in the HFE gene may have increased serum iron, transferrin saturation, and ferritin concentrations relative to individuals with the wild-type genotype. METHODS:We generated reference centiles for perc...

journal_title：Clinical chemistry

authors： Koziol JA,Ho NJ,Felitti VJ,Beutler E

更新日期：2001-10-01 00:00:00

abstract：:A method is presented for the assay of delta-aminolaevulinate dehydratase (EC 4.2.1.24) to be performed on 10 microL of whole blood. Results compare favorably with those obtained with an accepted method (J. Clin. Chem. Clin. Bio-chem. 12: 389-390, 1974) that involves use of 200 microL of blood. ...

journal_title：Clinical chemistry

authors： Wigfield DC,Farant JP

更新日期：1981-01-01 00:00:00

abstract：BACKGROUND:Mitochondrial diseases are clinically and genetically heterogeneous, with variable penetrance, expressivity, and differing age of onset. Disease-causing point mutations and large deletions in the mitochondrial genome often exist in a heteroplasmic state. Current molecular analyses require multiple different ...

journal_title：Clinical chemistry

authors： Zhang W,Cui H,Wong LJ

更新日期：2012-09-01 00:00:00

abstract：:I present the general pattern relationship between (a) plasma fibrinogen and cholinesterase activity and (b) plasma fibrinogen and hemoglobin, in 250 and 310 hospitalized adults, respectively. Although responses of cholinesterase were often unrelated to fibrinogen, when its activity in plasma was depressed, above-norm...

journal_title：Clinical chemistry

authors： Rice EW

更新日期：1977-01-01 00:00:00

abstract：:Development of a new, sensitive immunoassay for measuring transforming growth factor beta 1 (TGF-beta1) is described and compared with four commercially available TGF-beta1 immunoassays. Preanalytical conditions were evaluated. The nonlinearity found in serum or plasma is due to masking of TGF-beta1 by binding protein...

journal_title：Clinical chemistry

authors： Kropf J,Schurek JO,Wollner A,Gressner AM

更新日期：1997-10-01 00:00:00

abstract：BACKGROUND:There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS:Transferrin was iron-saturated by mixing the...

journal_title：Clinical chemistry

authors： Helander A,Husa A,Jeppsson JO

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:We used a difference in bias approach to evaluate the commutability of 4 frozen serum pools for 8 direct methods for measurement of HDL and LDL cholesterol (HDLC and LDLC). METHODS:Freshly collected nonfrozen sera from 138 diseased and 37 nondiseased patients and 4 frozen pools from the CDC Lipid Standardiz...

journal_title：Clinical chemistry

authors： Korzun WJ,Nilsson G,Bachmann LM,Myers GL,Sakurabayashi I,Nakajima K,Nakamura M,Shamburek RD,Remaley AT,Miller WG

更新日期：2015-08-01 00:00:00

abstract：BACKGROUND:Risk assessments of patients should be based on objective variables, such as biological markers that can be measured routinely. The acute response to stress causes the release of catecholamines from the adrenal medulla accompanied by chromogranin A (CGA). To date, no study has evaluated the prognostic value ...

journal_title：Clinical chemistry

authors： Zhang D,Lavaux T,Voegeli AC,Lavigne T,Castelain V,Meyer N,Sapin R,Aunis D,Metz-Boutigue MH,Schneider F

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:Recent studies have suggested that single-stranded DNA (ssDNA) library preparation can enrich short DNA species from the plasma of healthy individuals, cancer patients, and transplant recipients. Based on previous observations that fetal DNA molecules in the maternal plasma are shorter than maternal DNA mole...

journal_title：Clinical chemistry

authors： Vong JSL,Tsang JCH,Jiang P,Lee WS,Leung TY,Chan KCA,Chiu RWK,Lo YMD

更新日期：2017-05-01 00:00:00

abstract：:Agarose column chromatography has been used to separate plasma lipoproteins into very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL) and high-density lipoproteins (HDL). Applied to the diagnosis of primary type III hyperlipoproteinemia, the procedure is capable of demonstrating three characteristic an...

journal_title：Clinical chemistry

authors： Shepherd J,Packard CJ,Dryburgh FJ,Third JL

更新日期：1975-12-01 00:00:00

abstract：:We describe an enzyme-linked immunosorbent assay for determination of light-chain ratios for IgG, IgA, and IgM in serum. A commercial serum with known overall kappa and lambda concentrations was used as standard. To capture the respective immunoglobulins, we used antibodies to gamma, lambda and mu chains coated onto m...

journal_title：Clinical chemistry

authors： Chui SH,Lam CW,Lai KN

更新日期：1990-03-01 00:00:00