Abstract：
:In considering factors that might influence measurement of free thyroxin (T4), we evaluated the proportion (%) of T4 that could be stripped from thyroxin-binding globulin (TBG). The percentage of free T4 was measured in serially diluted sera from four normal subjects, four patients with hyperthyroidism or hypothyroidism, four pregnant women, and four malnourished subjects with low TBG. The critical percentage of stripping was determined by the product of the percentage free T4 and the critical dilution factor (the point where the percentage free T4 began to decrease). The mean values obtained for the respective patient groups--6.38%, 2.76%, 16.73%, 9.75%, and 4.28%--were proved to be related to the rate of saturation of TBG with T4. Values for percentage stripping determined with the "GammaCoat two-step RIA" and the "LiquiSol RIA" were well within the critical percentage stripping by equilibrium dialysis, except in the case of low-TBG serum as measured by LiquiSol RIA. Free T4 concentration as measured by LiquiSol RIA decreased as sample volume decreased. These findings were ascribed to the relatively high values for percentage stripping in the LiquiSol RIA, which led to erroneously low values for free T4.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Nakagawa T,Matsumura K,Takeda K,Shinoda N,Matsuda A,Matsushita T,Tagami Tsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
313-8issue
2eissn
0009-9147issn
1530-8561journal_volume
36pub_type
杂志文章abstract：:We describe a discrete automated radioimmunoassay system for determining choriomammotropin (placental lactogen) in human serum. With the present system it can be measured in as many as 37 unknown sera (50 muL) and three quality-control sera, in duplicate, within 1.5 h. The time required for sample preparation, incubat...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique. ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：BACKGROUND:Cardiac troponins are associated with cardiovascular risk in the general population, but whether temporal changes in cardiac troponin I provide independent prognostic information remains uncertain. Using a large community-based cohort with follow-up close to the present day, we aimed to investigate the assoc...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2018.301069
更新日期：2019-07-01 00:00:00
abstract：:Investigating the feasibility and validity of determining plasma ferritin concentration in blood obtained by finger prick, we studied 29 adults (ages 21-49 years) and 35 children (ages 14-66 months). Blood was sampled simultaneously in the same subject from both the antecubital vein (venous blood) and by finger pricki...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:We have measured by reversed-phase HPLC concentrations of amino acids in plasma in groups of 80 normal appropriate-weight term babies fed from birth either a casein formula (WhiteCap SMA, n = 26), a whey formula (Gold Cap SMA, n = 26), or breast milk (n = 28). They were studied from day 11 to week 15 postpartum. The t...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:Using "high performance" liquid chromatography, we studied non-protein-bound fractions and total concentrations of 18 solutes accumulating in sera from a group of 12 patients who were undergoing chronic ambulatory peritoneal dialysis (CAPD) and in predialysis sera from a group of 15 hemodialysis (HD) patients. We moni...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:We describe a method for radioimmunoassay of conjugated cholic acid, chenodeoxycholic acid, and deoxycholic acid in serum. In the method, 125I-labeled bile acid conjugates are used as the tracers along with antibodies raised against individual bile acid-bovine serum albumin conjugates. Antibody-bound and free bile aci...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：:A convenient screening procedure for presence of drugs of abuse in urine consists of two steps: adsorption of the drugs from urine onto a paper loaded with cation-exchange resin and detection of the adsorbed drugs by direct radioimmunoassay. The first step can be performed in the field, the second in a central laborat...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-10-01 00:00:00
abstract：:We compared the analytical performance of the Kodak Ektachem XR700 assays of iron (Fe) and total iron-binding capacity (TIBC) with that of a conventional ferrozine assay (performed with a Cobas-Bio) and occasionally with that of atomic-absorption spectrometry (AAS). The correlation was modest between Kodak and Cobas-B...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：BACKGROUND:False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation dif...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.091488
更新日期：2007-12-01 00:00:00
abstract：:We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filt...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:To validate azotemic markers as an index for intradialytic changes in solute concentration, we compared eight solutes (pseudouridine, xanthine, hypoxanthine, peak 4, peak 5, p-hydroxyhippuric acid, indoxyl sulfate, and hippuric acid) with five classical azotemic markers (urea, creatinine, uric acid, phosphate, and pot...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:The aim of this investigation was to study the distribution of arsenic species in human organs following fatal acute intoxication by arsenic trioxide. The collected autopsy samples of most organs were ground and dried, and the total arsenic was measured by electrothermal atomic absorption spectrometry (ETAAS). The ars...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:The potential errors of the one-point calibration technique are described mathematically. I examine the theoretical causes and effects of analytical errors in absorbance and of errors in determining calibration concentration, and discuss techniques for minimizing their impact. The dependence of these errors on the cal...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:We describe a "high-performance" liquid-chromatographic assay for measuring ritalinic acid in 300 muL of serum. The procedure includes adsorption of ritalinic acid and internal standard (alpha,alpha-dimethyl-beta-emthylsuccinimide) from serum onto charcoal, from which both compounds are eluted with methanol. The metha...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:We determined the urinary excretion of 5-fluorouracil (5FU) and its metabolites during six days of chemotherapy, using the non-invasive approach of 19F nuclear magnetic resonance. With this method, which requires no labeled drug, one can study the biological sample directly and simultaneously identify and quantify all...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Liquid scintillation counting of 3H-labeled whole-blood samples is severely impaired owing to quenching by blood pigments. In this study, dry oxidation and chemical solubilization followed by decolorization were the two general methods used to eliminate color quenching. Three blood volumes were examined: 0.25, 0.50, a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:Gene mutations responsible for the majority of Duchenne/Becker muscular dystrophy (DMD/BMD) and cystic fibrosis (CF) chromosomes have been identified. We describe a DNA-based strategy, rather than the traditional biochemical assays, for screening newborns. DNA sequences spanning the CF mutation and several DMD/BMD del...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：BACKGROUND:The use of nonstandardized N-terminal pro-B-type natriuretic peptide (NT-proBNP) assays can contribute to the misdiagnosis of heart failure (HF). Moreover, there is yet to be established a common consensus regarding the circulating forms of NT-proBNP being used in current assays. We aimed to characterize and...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2012.200204
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:A previously developed monoclonal/polyclonal ELISA (Mono/Poly) to detect plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in breast, prostate, and other cancers. Here we describe the clinical validation of a new dual monoclonal (Dual Mono) assay. We compared both assays ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.117465
更新日期：2009-05-01 00:00:00
abstract：:We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay cor...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Problems encountered in the uroporphyrinogen I synthase assay were found to be due to the Triton reagent, slit-width and excitation wavelength settings of the spectrophotofluorometer, and leg-phase in the enzyme reaction. Of Triton X-100 from four suppliers, only three were found suitable. Differences in enzyme activi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：BACKGROUND:Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. We studied the extent to which PMP subpopulations parallel platelet activation in vitro and in vivo. METHODS:Using flow cytometry, we analyzed PMP subpopulations from resting and activa...
journal_title：Clinical chemistry
pub_type： 临床试验,杂志文章
doi：10.1373/clinchem.2005.057414
更新日期：2006-04-01 00:00:00
abstract：:We describe a gas--liquid-chromatographic method for determining the concentration of amantadine hydrochloride in urine with beta-phenylethylamine as internal standard. The urine sample is made alkaline and extracted with 0.5 mL of chloroform. After centrifugation the aqueous layer is aspirated, and an aliquot of the ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
abstract：:Over the past decade, significant improvements in immunochemical and electrophoretic techniques have enabled collection of heretofore unavailable data on proteins in biological fluids, greatly increasing our understanding of protein physiology in the various body compartments and providing the foundation for clinical ...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1982-05-01 00:00:00
abstract：:We compared results for measurements of creatine kinase isoenzyme MB (CK-MB) by immunoinhibition vs immunoprecipitation, using sera from 53 normal healthy individuals, 55 patients with increased CK-MB associated with acute myocardial infarction, and 42 patients whose blood exhibited one or more abnormal forms of CK by...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:An optimized assay for alkaline phosphatase (EC 3.1.3.1) is reported. A discrete analyzer, the DuPont Automatic Clinical Analyzer (aca), was used for this study. The assay is based on results of response-surface experimental co-optimization techniques, and response is enhanced over the present aca assay. A key feature...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:In this simple, quick procedure for determining copper in human serum and urine, the serum and urine specimens were analyzed directly after dilution with a solution of HNO3 and Triton X-100, 1 mL/L each. We calibrated with aqueous standards for quantitation in Zeeman background atomic absorption spectrometry. By modif...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：BACKGROUND:Institutions face increasing charges related to molecular genetic testing for neurological diseases. The literature contains little information on the utilization and performance of these tests. METHODS:A retrospective utilization review was performed to determine the diagnostic yield of neurogenetic tests ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2006.083360
更新日期：2007-06-01 00:00:00
abstract：BACKGROUND:Reduced renal function is an established risk factor for cardiovascular events. We compared 3 measures of renal function--serum cystatin C, serum creatinine, and calculated creatinine clearance--as predictors of subsequent cardiovascular events in a community-based population of elderly individuals. METHODS...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.135962
更新日期：2010-05-01 00:00:00
