Abstract:
BACKGROUND:Rapid intraoperative parathyroid hormone (PTH) measurements are an important prerequisite for minimally invasive parathyroidectomy, serving as a feasible marker for "cure" because of the short half-life of PTH. Because automated analysis may facilitate monitoring, two automated PTH assays were compared with an established manual method. METHODS:We collected 109 plasma samples during minimally invasive surgery on 20 patients with primary hyperparathyroidism and single-gland disease. PTH was analyzed manually with a test from Nichols and by two automated assays from Diagnostic Product Corporation (DPC) and Roche, respectively. PTH half-life and residual concentrations were calculated by two kinetic models. RESULTS:Despite good overall correlations between methods [DPC = 1.07(Nichols) - 12 ng/L; r = 0.95, S(y/x) = 26 ng/L and Roche = 1.16(Nichols) - 2.82 ng/L; r = 0.98; S(y/x) = 16 ng/L], marked interindividual differences were observed. The iterative kinetic model failed with a nonuniform PTH decrease, but the interpolative model produced valid results. The mean (SD) half-life of 3.7 +/- 1.4 min with DPC differed significantly (P <0.05) from the 4.3 +/- 1.6 min with Roche (Nichols, 4.0 +/- 1.6 min). DPC produced significantly lower mean residual PTH (15 ng/L) vs Roche (27 ng/L); Nichols results were between them (20 ng/L). However, these differences were clinically irrelevant. CONCLUSIONS:Automated methods are as suitable as the manual test. The preoperative baseline PTH is necessary but is insufficient for kinetic calculations.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Bieglmayer C,Prager G,Niederle Bsubject
Has Abstractpub_date
2002-10-01 00:00:00pages
1731-8issue
10eissn
0009-9147issn
1530-8561journal_volume
48pub_type
杂志文章abstract::We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly sim...
journal_title:Clinical chemistry
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doi:
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abstract::In this simple, quick procedure for determining copper in human serum and urine, the serum and urine specimens were analyzed directly after dilution with a solution of HNO3 and Triton X-100, 1 mL/L each. We calibrated with aqueous standards for quantitation in Zeeman background atomic absorption spectrometry. By modif...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
更新日期:1983-08-01 00:00:00
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