Abstract：
BACKGROUND:MALDI-TOF mass spectrometry (MS) is set to make inroads into clinical chemistry because it offers advantages over other analytical platforms. These advantages include low acquisition and operating costs, ease of use, ruggedness, and high throughput. When coupled with innovative front-end strategies and applied to important clinical problems, it can deliver rapid, sensitive, and cost-effective assays. CONTENT:This review describes the general principles of MALDI-TOF MS, highlights the unique features of the platform, and discusses some practical methods based upon it. There is substantial potential for MALDI-TOF MS to make further inroads into clinical chemistry because of the selectivity of mass detection and its ability to independently quantify proteoforms. SUMMARY:MALDI-TOF MS has already transformed the practice of clinical microbiology and this review illustrates how and why it is now set to play an increasingly important role in in vitro diagnostics in particular, and clinical chemistry in general.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Duncan MW,Nedelkov D,Walsh R,Hattan SJdoi
10.1373/clinchem.2015.239491subject
Has Abstractpub_date
2016-01-01 00:00:00pages
134-43issue
1eissn
0009-9147issn
1530-8561pii
clinchem.2015.239491journal_volume
62pub_type
杂志文章,评审abstract：:We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%. The assay is simple, rapid, and inexpensive. Albumin excretion was 6.2 (SD 4.1) mg/...
journal_title：Clinical chemistry
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abstract：BACKGROUND:Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS:The CD...
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更新日期：1982-04-01 00:00:00
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更新日期：1988-04-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1989-06-01 00:00:00
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doi：
更新日期：1990-02-01 00:00:00
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更新日期：1998-06-01 00:00:00
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doi：
更新日期：1994-02-01 00:00:00
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doi：
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doi：
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doi：
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更新日期：2002-11-01 00:00:00
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doi：
更新日期：1990-08-01 00:00:00
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更新日期：1979-04-01 00:00:00
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doi：
更新日期：1999-09-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1993-04-01 00:00:00
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doi：
更新日期：1983-12-01 00:00:00
