Abstract:
BACKGROUND:The liver is frequently subject to insult because of viral infection, alcohol abuse, or toxic chemical exposure. Extensive research has been conducted to identify blood markers that can better discern liver damage, but little progress has been achieved in clinical practice. Recently, circulating microRNAs (miRNAs) have been reported as potential biomarkers for the noninvasive diagnosis of cancer. In this study, we investigated whether plasma miRNAs have diagnostic utility in identifying liver disease. METHODS:The study was divided into 2 phases: marker selection by real-time quantitative PCR analysis of a small set of plasma samples, and marker validation with a large set of plasma samples from 83 patients with chronic hepatitis B viral infections, 15 patients with skeletal muscle disease, and 40 healthy controls. Two mouse model systems, d-galactosamine- and alcohol-induced liver injury, were also developed to evaluate whether differences in miRNA concentration were associated with various liver diseases. RESULTS:Among the miRNA candidates identified, miR-122 presented a disease severity-dependent change in plasma concentration in the patients and animal models. Compared with an increase in aminotransferase activity in the blood, the change in miR-122 concentration appeared earlier. Furthermore, this change was more specific for liver injury than for other organ damage and was more reliable, because the change was correlated with liver histologic stage. CONCLUSIONS:Our findings suggest that circulating miR-122 has potential as a novel, predictive, and reliable blood marker for viral-, alcohol-, and chemical-induced liver injury.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Zhang Y,Jia Y,Zheng R,Guo Y,Wang Y,Guo H,Fei M,Sun Sdoi
10.1373/clinchem.2010.147850subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1830-8issue
12eissn
0009-9147issn
1530-8561pii
clinchem.2010.147850journal_volume
56pub_type
杂志文章abstract:BACKGROUND:Thrombopoietin (TPO), the major hormone controlling platelet production, has been measured in thrombocytopenias with discordant results. The aim of our work was to assess the value of the TPO assay for differential diagnosis of thrombocytopenias in a large cohort of patients classified according to the resul...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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journal_title:Clinical chemistry
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doi:10.1373/clinchem.2005.059568
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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pub_type: 临床试验,杂志文章
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 历史文章,杂志文章
doi:
更新日期:2002-03-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1995-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
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doi:
更新日期:1997-11-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.082081
更新日期:2007-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1994-07-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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doi:
更新日期:1993-04-01 00:00:00
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doi:
更新日期:2000-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1994-11-01 00:00:00
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