Buprenorphine and norbuprenorphine in hair of pregnant women and their infants after controlled buprenorphine administration.

abstract：:We present a simple method for subclass typing of IgG paraproteins, with which we have demonstrated a large number of paraproteins that were undetected by conventional immunofixation techniques. The types and distribution of IgG subclass paraproteins were analyzed in 92 human sera in which IgG paraproteins had been de...

journal_title：Clinical chemistry

authors： Klouche M,Bradwell AR,Wilhelm D,Kirchner H

更新日期：1995-10-01 00:00:00

abstract：:A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after ...

journal_title：Clinical chemistry

authors： Egberts J,Soederhuizen W,Gebhardt DO

更新日期：1983-02-01 00:00:00

abstract：:The increasing use of monoclonal antibodies (MAbs) for disease diagnosis and therapy has created a class of patients at risk for systematic error in clinical testing due to interference by human anti-murine antibodies (HAMA). HAMA interference is often difficult to detect and can cause either an increase or a decrease...

journal_title：Clinical chemistry

authors： Morrissey NE,Quadri SF,Kinders R,Brigham C,Rose S,Blend MJ

更新日期：1993-03-01 00:00:00

abstract：:We tested 231 urine samples by six immunoassay methods--EMIT d.a.u., EMIT II, Roche Abuscreen Online, Abbott TDx, Diagnostic Products Corp. (DPC) double-antibody radioimmunoassay (RIA), and Biosite Triage--and by negative chemical ionization gas chromatography/mass spectrometry to determine how the immunoassays perfor...

journal_title：Clinical chemistry

authors： Fitzgerald RL,Rexin DA,Herold DA

更新日期：1994-03-01 00:00:00

abstract：BACKGROUND:The gene that causes most cases of hereditary hemochromatosis is designated HFE. Individuals with mutations in the HFE gene may have increased serum iron, transferrin saturation, and ferritin concentrations relative to individuals with the wild-type genotype. METHODS:We generated reference centiles for perc...

journal_title：Clinical chemistry

authors： Koziol JA,Ho NJ,Felitti VJ,Beutler E

更新日期：2001-10-01 00:00:00

abstract：:A one-stage semiautomated method for estimating total phospholipids in plasma is reported. The phospholipids are extracted into aqueous ethanol, combined with a Lewis acid, and the change in pH is monitored colorimetrically. The method, compared with the acid digestion method of Gomori [J. Lab. Clin. Med. 27, 955 (194...

journal_title：Clinical chemistry

authors： Wootton DG

更新日期：1977-09-01 00:00:00

abstract：BACKGROUND:Sensitive and specific biomarkers for the early detection of esophageal squamous cell carcinoma (ESCC) are urgently needed to reduce the high morbidity and mortality of the disease. The discovery of serum microRNAs (miRNAs) and their unique concentration profiles in patients with various diseases makes them ...

journal_title：Clinical chemistry

authors： Zhang C,Wang C,Chen X,Yang C,Li K,Wang J,Dai J,Hu Z,Zhou X,Chen L,Zhang Y,Li Y,Qiu H,Xing J,Liang Z,Ren B,Yang C,Zen K,Zhang CY

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Noninvasive prenatal testing (NIPT) uses cell-free DNA (cfDNA) as an analyte to detect copy-number alterations in the fetal genome. Because maternal and fetal cfDNA contributions are comingled, changes in the maternal genome can manifest as abnormal NIPT results. Circulating tumor DNA (ctDNA) present in case...

journal_title：Clinical chemistry

authors： Dharajiya NG,Grosu DS,Farkas DH,McCullough RM,Almasri E,Sun Y,Kim SK,Jensen TJ,Saldivar JS,Topol EJ,van den Boom D,Ehrich M

更新日期：2018-02-01 00:00:00

abstract：:We describe a procedure for determining amitriptyline and imipramine and their active metabolites nortriptyline and desipramine, respectively, at therapeutic concentrations in human plasma by use of liquid chromatography. The drugs are extracted at pH 10.5 into hexane/isoamyl alcohol, which is evaporated and the resid...

journal_title：Clinical chemistry

authors： Vandemark FL,Adams RF,Schmidt GJ

更新日期：1978-01-01 00:00:00

abstract：:We report here the normal range of serum alkaline phophatase activity as measured by the method proposed by Hausamen et al. [Clin. Chim. Acta 15, 241 (1967)] with a much larger sample size than used in previous investigations. In the statistical analysis the sample population is subdivided by sex and age, two variable...

journal_title：Clinical chemistry

authors： Eastman JR,Bixler D

更新日期：1977-09-01 00:00:00

abstract：:Clinicians experience difficulty in correctly interpreting the results of in vitro thyroid function tests in the presence of abnormalities of thyrobinding proteins or when results are borderline. This difficulty has been largely resolved in our laboratory by three innovations. First, the borderline areas for each of t...

journal_title：Clinical chemistry

authors： Pain RW,Duncan BM

更新日期：1976-10-01 00:00:00

abstract：:We have developed and validated simple, rapid immunoassays to measure concentrations of albumin in urine ranging from 5 to 200 mg/L. We use antiserum to human albumin, raised in sheep, and we separate the antibody-bound and free fractions of albumin by using a second antiserum, produced in donkeys, against the Fc frag...

journal_title：Clinical chemistry

authors： Silver A,Dawnay A,Landon J,Cattell WR

更新日期：1986-07-01 00:00:00

abstract：:This HPLC/immunoassay procedure measures digoxin in serum with no interference from digoxin metabolites or digoxin-like factors. We used solid-phase (C18 and Diol) extraction, a C18 column with a tetrahydrofuran/water mobile phase, and final quantification by fluorescence polarization immunoassay. Deslanoside and gito...

journal_title：Clinical chemistry

authors： Stone JA,Soldin SJ

更新日期：1988-12-01 00:00:00

abstract：BACKGROUND:Distinguishing adenocarcinoma and squamous cell carcinoma subtypes of non-small cell lung cancers is critical to patient care. Preoperative minimally-invasive biopsy techniques, such as fine needle aspiration (FNA), are increasingly used for lung cancer diagnosis and subtyping. Yet, histologic distinction of...

journal_title：Clinical chemistry

authors： Bensussan AV,Lin J,Guo C,Katz R,Krishnamurthy S,Cressman E,Eberlin LS

更新日期：2020-11-01 00:00:00

abstract：:We report here the evaluation of the Abbott TDx assay with a monoclonal antibody for selectively quantifying cyclosporine (CsA) in whole blood. Over the clinically relevant concentration ranges, results with this assay demonstrated within- and between-run CVs of less than 2.5% and 5%, respectively; sensitivity of 25 m...

journal_title：Clinical chemistry

authors： Yatscoff RW,Copeland KR,Faraci CJ

更新日期：1990-11-01 00:00:00

abstract：BACKGROUND:Prostate-specific antigen (PSA) is a valuable prostatic cancer biomarker that is now widely used for population screening, diagnosis, and monitoring of patients with prostate cancer. Despite the voluminous literature on this biomarker, relatively few reports have addressed the issue of its physiological func...

journal_title：Clinical chemistry

authors： Diamandis EP

更新日期：2000-07-01 00:00:00

abstract：BACKGROUND:Previous studies have shown a single linear relationship between mean plasma glucose (MPG) and hemoglobin A(1c) (HbA(1c)). We examined the relationship in different treatment groups of patients with type 1 diabetes participating in the Diabetes Control and Complications Trial (DCCT). METHODS:Seven-point glu...

journal_title：Clinical chemistry

authors： Kilpatrick ES,Rigby AS,Atkin SL

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Paternal zygosity testing is used for determining homo- or hemizygosity of RHD in pregnancies that are at a risk of hemolytic disease of the fetus and newborn. At present, this is achieved by using real-time PCR or the Rhesus box PCR, which can be difficult to interpret and unreliable, particularly for black...

journal_title：Clinical chemistry

authors： Sillence KA,Halawani AJ,Tounsi WA,Clarke KA,Kiernan M,Madgett TE,Avent ND

更新日期：2017-08-01 00:00:00

abstract：:To obtain the best estimates of the average intraindividual biological variability (CVb) in the concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), high-density lipoprotein cholesterol (HDLC), and triglyceride serum lipids in a person's blood, we evaluated results from 30 studies publ...

journal_title：Clinical chemistry

authors： Smith SJ,Cooper GR,Myers GL,Sampson EJ

更新日期：1993-06-01 00:00:00

abstract：BACKGROUND:Immunoassays specific for 1-84 parathyroid hormone (PTH) reportedly reflect the bioactivity of PTH; however, PTH immunoassays can be susceptible to interference by cross-reacting PTH fragments. In addition, these assays currently lack standardization. A methodology using immunocapture purification with liqui...

journal_title：Clinical chemistry

authors： Kumar V,Barnidge DR,Chen LS,Twentyman JM,Cradic KW,Grebe SK,Singh RJ

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND:Detection of circulating tumor cells (CTCs) in the peripheral blood is a rapidly developing research field with clear clinical implications for the staging and monitoring of cancer patients. Current CTC assays, including the US Food and Drug Administration-cleared CellSearch® system, typically use markers [e...

journal_title：Clinical chemistry

authors： Pantel K,Denève E,Nocca D,Coffy A,Vendrell JP,Maudelonde T,Riethdorf S,Alix-Panabières C

更新日期：2012-05-01 00:00:00

abstract：:Development of a new, sensitive immunoassay for measuring transforming growth factor beta 1 (TGF-beta1) is described and compared with four commercially available TGF-beta1 immunoassays. Preanalytical conditions were evaluated. The nonlinearity found in serum or plasma is due to masking of TGF-beta1 by binding protein...

journal_title：Clinical chemistry

authors： Kropf J,Schurek JO,Wollner A,Gressner AM

更新日期：1997-10-01 00:00:00

abstract：BACKGROUND:FMR1 full mutations (FMs) (CGG expansion >200) in males mosaic for a normal (<45 CGG) or gray-zone (GZ) (45-54 CGG) allele can be missed with the standard 2-step fragile X syndrome (FXS) testing protocols, largely because the first-line PCR tests showing a normal or GZ allele are not reflexed to the second-l...

journal_title：Clinical chemistry

authors： Aliaga SM,Slater HR,Francis D,Du Sart D,Li X,Amor DJ,Alliende AM,Santa Maria L,Faundes V,Morales P,Trigo C,Salas I,Curotto B,Godler DE

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:An assay using a mouse antisialyl Lewis X (sLeX) antibody (CSLEX-1) is used clinically for screening and monitoring patients with breast cancer in Japan. However, the IgM isoform of CSLEX-1 is not preferred for the assay because the bulkiness of IgM generally causes poor accessibility to the antigen. To solv...

journal_title：Clinical chemistry

authors： Yamashita J,Kobayashi I,Tatematsu K,Sezutsu H,Noda K,Ishihara H

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Gradient gel electrophoresis (GGE) and nuclear magnetic resonance (NMR) spectroscopy are both widely accepted methods for measuring LDL and HDL particle size. However, whether or not GGE- or NMR-measured LDL or HDL particle size predicts coronary heart disease (CHD) risk to a similar extent is currently unkn...

journal_title：Clinical chemistry

authors： Arsenault BJ,Lemieux I,Després JP,Wareham NJ,Stroes ES,Kastelein JJ,Khaw KT,Boekholdt SM

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:Cleavage of conjugates is an important step in toxicological analysis, especially of urine samples. The aim of this study was to combine the advantages and to reduce the disadvantages of acid hydrolysis and conventional enzymatic hydrolysis procedures. METHODS:beta-Glucuronidase (GRD; EC 3.2.1.31) and aryls...

journal_title：Clinical chemistry

authors： Toennes SW,Maurer HH

更新日期：1999-12-01 00:00:00

abstract：:We describe the development of a stable reference material for prostatic acid phosphatase, derived from human prostatic tissue and human seminal fluid. The enzyme was purified by an L-tartramic acid affinity-chromatography technique. Two-dimensional electrophoresis revealed essentially no contaminating proteins, and s...

journal_title：Clinical chemistry

authors： Duncan PH,Van Etten RL,MacNeil ML,Shaw LM

更新日期：1984-08-01 00:00:00

abstract：BACKGROUND:Improved methods are required to screen drug candidates for their influences on protein interactions. There is also a compelling need for miniaturization of screening assays, with attendant reductions in reagent consumption and assay costs. METHODS:We used sensitive, miniaturized proximity ligation assays (...

journal_title：Clinical chemistry

authors： Gustafsdottir SM,Wennström S,Fredriksson S,Schallmeiner E,Hamilton AD,Sebti SM,Landegren U

更新日期：2008-07-01 00:00:00

abstract：:We assessed the LKB "Delfia" (time-resolved dissociation-enhanced lanthanide fluoroimmunoassay) and the Amersham "Amerlite" (enhanced luminescent immunometry) assays of thyrotropin in serum. Both assays are sensitive (respective detection limits: 0.02 and 0.04 milli-int. unit/L) and have very good within- and between-...

journal_title：Clinical chemistry

authors： Parnham AJ,Tarbit IF

更新日期：1987-08-01 00:00:00

abstract：BACKGROUND:Carcinoembryonic antigen (CEA) is one of the most widely used tumor markers worldwide. Its main application is mostly in gastrointestinal cancers, especially in colorectal malignancy. Although in use for almost 30 years, the clinical value of CEA in colorectal cancer is still not clear. METHODS:The literatu...

journal_title：Clinical chemistry

authors： Duffy MJ

更新日期：2001-04-01 00:00:00