Abstract:
:Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is a recessively inherited defect in the mitochondrial beta-oxidation of fatty acids. A single nucleotide change, the A985 --> G transition, in the MCAD gene accounts for approximately 90% of all the disease-causing mutations in the patients. We have used PCR to amplify a segment of the human MCAD gene and typed the allelic sequence variation at base 985 by a colorimetric oligonucleotide ligation assay (OLA). PCR/OLA provides a technique that permits differentiation of the homozygotes, heterozygotes, and normals for the A985 --> G allele in the MCAD gene. Genotyping of 1908 random Finnish DNA samples by OLA identified 10 carriers of the mutant allele, but no homozygotes were found. The calculated carrier frequency for the A985 --> G mutation was 1:191 (95% confidence limits, 1:118-1:501), and the calculated frequency for the A985 --> G homozygotes was 1:147,000 (95% confidence limits, 1:56,000-1:1,004,000).
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Romppanen EL,Mononen T,Mononen Isubject
Has Abstractpub_date
1998-01-01 00:00:00pages
68-71issue
1eissn
0009-9147issn
1530-8561journal_volume
44pub_type
杂志文章abstract::A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
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更新日期:1999-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1976-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-05-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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pub_type: 临床试验,杂志文章
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journal_title:Clinical chemistry
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