Abstract：
BACKGROUND:The detection of monoclonal free light chains (FLCs) is an important diagnostic aid for a variety of monoclonal gammopathies and is especially important in light-chain diseases, such as light-chain myeloma, primary systemic amyloidosis, and light-chain-deposition disease. These diseases are more prevalent in the elderly, and assays to detect and quantify abnormal amounts of FLCs require reference intervals that include elderly donors. METHODS:We used an automated immunoassay for FLCs and sera from a population 21-90 years of age. We used the calculated reference and diagnostic intervals to compare FLC results with those obtained by immunofixation (IFE) to detect low concentrations of monoclonal kappa and lambda FLCs in the sera of patients with monoclonal gammopathies. RESULTS:Serum kappa and lambda FLCs increased with population age, with an apparent change for those >80 years. This trend was lost when the FLC concentration was normalized to cystatin C concentration. The ratio of kappa FLC to lambda FLC (FLC K/L) did not exhibit an age-dependent trend. The diagnostic interval for FLC K/L was 0.26-1.65. The 95% reference interval for kappa FLC was 3.3-19.4 mg/L, and that for lambda FLC was 5.7-26.3 mg/L. Detection and quantification of monoclonal FLCs by nephelometry were more sensitive than IFE in serum samples from patients with primary systemic amyloidosis and light-chain-deposition disease. CONCLUSIONS:Reference and diagnostic intervals for serum FLCs have been developed for use with a new, automated immunoassay that makes the detection and quantification of monoclonal FLCs easier and more sensitive than with current methods. The serum FLC assay complements IFE and allows quantification of FLCs in light-chain-disease patients who have no detectable serum or urine M-spike.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Katzmann JA,Clark RJ,Abraham RS,Bryant S,Lymp JF,Bradwell AR,Kyle RAsubject
Has Abstractpub_date
2002-09-01 00:00:00pages
1437-44issue
9eissn
0009-9147issn
1530-8561journal_volume
48pub_type
杂志文章abstract：BACKGROUND:Insulin, growth hormone (GH), and growth factors (insulin-like growth factors [IGFs] and their binding proteins [IGFBPs]) are known to influence fetal growth and also the synthesis/secretion of the recently discovered hormones leptin and ghrelin. METHODS:In 153 delivering mothers and their offspring at birt...
journal_title：Clinical chemistry
pub_type： 杂志文章
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doi：
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abstract：:We tested a new amylase reagent involving chromogenic substrates (Pantrak, Calbiochem-Behring) for precision, dynamic range, and susceptibility to potential interferences. Two p-nitrophenyl-alpha-maltaosides are used as substrates, which amylase cleaves to shorter-chain p-nitrophenyl maltaosides, the latter then yield...
journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
更新日期：1982-04-01 00:00:00
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doi：
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journal_title：Clinical chemistry
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doi：
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doi：
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doi：
更新日期：1988-12-01 00:00:00
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1984-05-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1978-11-01 00:00:00
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doi：
更新日期：1997-10-01 00:00:00
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doi：
更新日期：1994-11-01 00:00:00
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doi：
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doi：
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doi：
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更新日期：1999-10-01 00:00:00
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