Hemoglobin Görwihl [alpha2beta(2)5(A2)Pro-->Ala], an electrophoretically silent variant with impaired glycation.

abstract：BACKGROUND:Mutations in the retina-specific ABC transporter (ABCA4) gene have been associated with several forms of macular degenerations. Because the high complexity of the molecular genotype makes scanning of the ABCA4 gene cumbersome, we describe here the first use of denaturing HPLC (DHPLC) to screen for ABCA4 muta...

journal_title：Clinical chemistry

authors： Stenirri S,Fermo I,Battistella S,Galbiati S,Soriani N,Paroni R,Manitto MP,Martina E,Brancato R,Allikmets R,Ferrari M,Cremonesi L

更新日期：2004-08-01 00:00:00

abstract：:In this rapid, cost-effective, enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone (17-OHP) eluted from dried blood spotted on filter paper, second antibody is coated onto the microwell plate and horseradish peroxidase (EC 1.11.1.7) is the label enzyme. Antiserum to 17-OHP was prepared by using 4-(2-car...

journal_title：Clinical chemistry

authors： Maeda M,Arakawa H,Tsuji A,Yamagami Y,Isozaki A,Takahashi T,Haruki E

更新日期：1987-06-01 00:00:00

abstract：:By using bivariate probability estimation for the diagnosis of acute myocardial infarction (AMI) we show how to overcome the difficulties encountered for patients whose clinical presentation is atypical and those encountered when multiple isoenzyme determinations are treated by univariate methods. We use the values fo...

journal_title：Clinical chemistry

authors： Bernstein LH,Good IJ,Holtzman GI,Deaton ML,Babb J

更新日期：1989-03-01 00:00:00

abstract：BACKGROUND:Extracellular matrix alterations are important elements in the arterial changes seen in diabetes, being associated with increased vascular stiffness and the development of cardiovascular diseases. However, no biomarkers for diabetes-related arterial changes have been defined. METHODS:Mammary artery specimen...

journal_title：Clinical chemistry

authors： Cangemi C,Skov V,Poulsen MK,Funder J,Twal WO,Gall MA,Hjortdal V,Jespersen ML,Kruse TA,Aagard J,Parving HH,Knudsen S,Høilund-Carlsen PF,Rossing P,Henriksen JE,Argraves WS,Rasmussen LM

更新日期：2011-11-01 00:00:00

abstract：:We present a unified approach to the use of linear models and matrix least squares with the intention of providing a better understanding of the techniques themselves and of the statistics that arise from these techniques as they are used in clinical chemistry. Emphasis is placed on the importance of appropriate exper...

journal_title：Clinical chemistry

authors： Deming SN,Morgan SL

更新日期：1979-06-01 00:00:00

abstract：:Measurement of acetaminophen by analysis of the second derivative of its spectrum is specific and sensitive. The method of extraction and the use of just one phosphate buffer as reagent makes this method very convenient. Readings are reliable from 10 to 1500 mg/L. A turnaround time of 20 min makes this method well sui...

journal_title：Clinical chemistry

authors： Dingeon B,Charvin MA,Quenard MT,Thome H

更新日期：1988-06-01 00:00:00

abstract：BACKGROUND:Advances in the biological sciences and technology are providing molecular targets for diagnosing and treating cancer. Current classifications in surgical pathology for staging malignancies are based primarily on anatomic features (e.g., tumor-node-metastasis) and histopathology (e.g., grade). Microarrays to...

journal_title：Clinical chemistry

authors： Bernard PS,Wittwer CT

更新日期：2002-08-01 00:00:00

abstract：:We coupled a kinetic procedure to a selective inhibiting method for determining amylase isoenzymes in biological samples, using 4-nitrophenylmaltopentaoside plus 4-nitrophenylmaltohexaoside as substrate and a wheat-germ selective inhibitor with the Gilford S-III spectrophotometer. On plotting remaining amylase activit...

journal_title：Clinical chemistry

authors： Jiménez A,Arenas J,Santos I,Martínez A

更新日期：1986-08-01 00:00:00

abstract：:Creatine kinase isoenzyme BB (CK-BB) is found in the serum of patients with various types of cancer. Using both radioimmunoassay and agarose electrophoresis, we found abnormal amounts of this isoenzyme in the serum of 15 of 17 patients with untreated prostatic carcinoma. Among patients with other types of adenocarcino...

journal_title：Clinical chemistry

authors： Silverman LM,Dermer GB,Zweig MH,Van Steirteghem AC,Tökés ZA

更新日期：1979-08-01 00:00:00

abstract：BACKGROUND:Patients differ in responses to warfarin, which is commonly prescribed to treat thromboembolic events. Genetic variations in the cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9), vitamin K epoxide reductase complex, subunit 1 (VKORC1), and gamma-glutamyl carboxylase (GGCX) genes have been shown...

journal_title：Clinical chemistry

authors： Rai AJ,Udar N,Saad R,Fleisher M

更新日期：2009-04-01 00:00:00

abstract：:We evaluated superoxide dismutase (EC 1.15.1.1) and glutathione peroxidase (EC 1.11.1.9) activities in the erythrocytes of lead-exposed and nonexposed workers from distinct industries localized in the State of São Paulo, Brazil. Concentrations of either lead or protoporphyrin IX, or both, in blood were used to indicat...

journal_title：Clinical chemistry

authors： Monteiro HP,Abdalla DS,Arcuri AS,Bechara EJ

更新日期：1985-10-01 00:00:00

abstract：:To see if saliva is a valid substitute for plasma in assay of progesterone even when concentrations of hormone and binding proteins are fluctuating, we determined the concentrations of total and free progesterone in plasma and salivary progesterone in specimens from 36 women volunteers during the course of pregnancy a...

journal_title：Clinical chemistry

authors： Meulenberg PM,Hofman JA

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step. METHODS:We evaluated the sensitivity and specificity of homogeneous scanning, using a saturatin...

journal_title：Clinical chemistry

authors： Reed GH,Wittwer CT

更新日期：2004-10-01 00:00:00

abstract：BACKGROUND:alpha(1)-Antitrypsin is the major plasma serine protease inhibitor. Its deficiency is mainly associated with the alleles PI*S and PI*Z and can lead to obstructive lung disease in adults and to liver cirrhosis during childhood. METHODS:A multiplex PCR method has been established that uses two sets of primers...

journal_title：Clinical chemistry

authors： von Ahsen N,Oellerich M,Schütz E

更新日期：2000-02-01 00:00:00

abstract：BACKGROUND:Identifying novel risk markers in cardiovascular patients remains a research priority. Longer follow-up generally is considered favorable in such studies, but associations of interest may become attenuated with increasing follow-up. This issue has not been adequately addressed in the context of patient cohor...

journal_title：Clinical chemistry

authors： Breitling LP,Mons U,Hahmann H,Koenig W,Rothenbacher D,Brenner H

更新日期：2017-03-01 00:00:00

abstract：:Diminished ability to utilize acetate (acetate intolerance) developed in a male patient on chronic hemodialysis after five years of maintenance dialysis. His ability to utilize lactate was also subnormal. We studied acetate metabolism in vitro by isolating lymphocytes from the patient's blood before dialysis and measu...

journal_title：Clinical chemistry

authors： Egan JD,Wells IC

更新日期：1979-03-01 00:00:00

abstract：:The clinical relevance of likelihood ratios (L-values) for revising the physician's diagnostic probabilities has been recognized. However, the calculation of L-values, particularly in the case of quantitative or mixed quantitative-binary test results, raises problems that have not yet been addressed. Based on a very g...

journal_title：Clinical chemistry

authors： Albert A

更新日期：1982-05-01 00:00:00

abstract：BACKGROUND:Serial measurements of neurohormones have been shown to improve prognostication in the setting of acute heart failure (HF) or chronic HF without therapeutic intervention. We investigated the prognostic role of serial measurements of emerging neurohormones and BNP in a cohort of chronic HF patients undergoing...

journal_title：Clinical chemistry

authors： Neuhold S,Huelsmann M,Strunk G,Struck J,Adlbrecht C,Gouya G,Elhenicky M,Pacher R

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Prostate-specific antigen (PSA) occurs in serum both free and in complex with protease inhibitors. The complex with alpha1-antichymotrypsin (ACT) is the major form in serum, and the proportion of PSA-ACT is higher in prostate cancer (PCa) than in benign prostatic hyperplasia (BPH). PSA also forms a complex w...

journal_title：Clinical chemistry

authors： Zhang WM,Finne P,Leinonen J,Vesalainen S,Nordling S,Stenman UH

更新日期：1999-06-01 00:00:00

abstract：BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...

journal_title：Clinical chemistry

authors： Ryu S,Chang Y,Kim DI,Kim WS,Suh BS

更新日期：2007-01-01 00:00:00

abstract：:We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measureme...

journal_title：Clinical chemistry

authors： Rautela GS,Liedtke RJ

更新日期：1978-01-01 00:00:00

abstract：BACKGROUND:Intraventricular hemorrhage (IVH) is a major cause of neurologic disabilities in preterm newborns. We evaluated the use of plasma activin A concentrations to predict the development of perinatal IVH. METHODS:We measured nucleated erythrocyte (NRBC) counts, plasma activin A, hypoxanthine (Hyp), and xanthine ...

journal_title：Clinical chemistry

authors： Florio P,Perrone S,Luisi S,Vezzosi P,Longini M,Marzocchi B,Petraglia F,Buonocore G

更新日期：2006-08-01 00:00:00

abstract：BACKGROUND:The differential diagnosis of D-2-hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) can be accomplished only by the measurement of the corresponding 2-hydroxyglutarate (2-HG). Available methods for the determination of D- a...

journal_title：Clinical chemistry

authors： Struys EA,Jansen EE,Verhoeven NM,Jakobs C

更新日期：2004-08-01 00:00:00

abstract：:To evaluate a theoretical model for calculating free thyroid hormones, based on the law of mass action, we compared values for both calculated and measured free thyroxin and free triiodothyronine in a group of normal subjects. To determine whether the concentrations of circulating free hormones were also predictable f...

journal_title：Clinical chemistry

authors： Fresco G,Curti G,Biggi A,Fontana B

更新日期：1982-06-01 00:00:00

abstract：:A monoclonal-antibody-based competitive radioimmunoassay for measuring human protein C is reported. With use of a purified protein C standard, the solid-phase assay was sensitive to less than 80 micrograms of protein C per liter. Intraassay CVs ranged from 5% to 8%; the inter-assay CV was 5.4%. Analytical recovery ave...

journal_title：Clinical chemistry

authors： Howard PR,Bovill EG,Mann KG,Tracy RP

更新日期：1988-02-01 00:00:00

abstract：BACKGROUND:Coffee consumption has been associated with several risk factors for coronary heart disease, including increased cholesterol, increased blood pressure, and increased plasma total homocysteine (tHcy). tHcy is determined by several B-vitamins. However, reports about the association between coffee intake and B-...

journal_title：Clinical chemistry

authors： Ulvik A,Vollset SE,Hoff G,Ueland PM

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:The optimal situation in external quality assessment (EQA) is to use commutable materials. No previous study has examined the commutability of a whole-blood material for point-of-care (POC) testing. The aim of this study was to determine the commutability of the Norwegian Quality Improvement of Laboratory Ex...

journal_title：Clinical chemistry

authors： Bukve T,Sandberg S,Vie WS,Sølvik U,Christensen NG,Stavelin A

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:Neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years and is characterized by a wide clinical and biological heterogeneity, from spontaneously regressive forms to cancers with a rapid and fatal progression. MYCN oncogene amplification is considered the most important p...

journal_title：Clinical chemistry

authors： Raggi CC,Bagnoni ML,Tonini GP,Maggi M,Vona G,Pinzani P,Mazzocco K,De Bernardi B,Pazzagli M,Orlando C

更新日期：1999-11-01 00:00:00

abstract：:In galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. We describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. Galactose is oxidized by galactose oxidase to D-galacto-hexadialdos...

journal_title：Clinical chemistry

authors： Mason GA,Summer GK,Dutton HH,Schwaner RC Jr

更新日期：1977-06-01 00:00:00

abstract：BACKGROUND:The Dutch project "Calibration 2000" aims at harmonization of laboratory results via calibration by development of matrix-based secondary reference materials. We considered the selection, preparation, and characterization of 34 potential reference materials (PRMs). METHODS:Sixteen PRMs were prepared either ...

journal_title：Clinical chemistry

authors： Cobbaert C,Weykamp C,Baadenhuijsen H,Kuypers A,Lindemans J,Jansen R

更新日期：2002-09-01 00:00:00