abstract：BACKGROUND:We sought to develop a tandem mass spectrometry assay in which the enzymatic activities of 3 lysosomal enzymes (α-glucosidase, α-galactosidase A, and α-l-iduronidase) could be quantified in dried blood spots by using a single assay buffer. METHODS:A 3-mm dried blood spot punch was incubated in a single assa...

journal_title：Clinical chemistry

authors： Duffey TA,Bellamy G,Elliott S,Fox AC,Glass M,Turecek F,Gelb MH,Scott CR

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Sensitive and specific biomarkers for the early detection of esophageal squamous cell carcinoma (ESCC) are urgently needed to reduce the high morbidity and mortality of the disease. The discovery of serum microRNAs (miRNAs) and their unique concentration profiles in patients with various diseases makes them ...

journal_title：Clinical chemistry

authors： Zhang C,Wang C,Chen X,Yang C,Li K,Wang J,Dai J,Hu Z,Zhou X,Chen L,Zhang Y,Li Y,Qiu H,Xing J,Liang Z,Ren B,Yang C,Zen K,Zhang CY

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:The liver is frequently subject to insult because of viral infection, alcohol abuse, or toxic chemical exposure. Extensive research has been conducted to identify blood markers that can better discern liver damage, but little progress has been achieved in clinical practice. Recently, circulating microRNAs (m...

journal_title：Clinical chemistry

authors： Zhang Y,Jia Y,Zheng R,Guo Y,Wang Y,Guo H,Fei M,Sun S

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Standardization of clinical measurements of C-reactive protein (CRP) is based on the availability of reference materials. The serum protein reference material ERM-DA470 was used as a calibrant for various commercially available immunoassays but has now been exhausted. The recently released ERM-DA470k/IFCC wa...

journal_title：Clinical chemistry

authors： Rzychon M,Zegers I,Schimmel H

更新日期：2010-09-01 00:00:00

abstract：BACKGROUND:We evaluated the analytical performance of 4 cystatin C assays (Siemens N Latex on BNII, Roche Tina-quant on Cobas c501, Genzyme on Cobas c501, and Tosoh ST AIA-PACK on Tosoh AIA-600II) according to guidelines published by the Clinical and Laboratory Standards Institute. METHODS:We evaluated total imprecisi...

journal_title：Clinical chemistry

authors： Li J,Dunn W,Breaud A,Elliott D,Sokoll LJ,Clarke W

更新日期：2010-08-01 00:00:00

abstract：BACKGROUND:Myeloperoxidase (MPO) is a biomarker of inflammation and oxidative stress produced by neutrophils, monocytes, and endothelial cells. Concentrations of MPO predict mortality in patients with chronic heart failure. This study sought to investigate the diagnostic accuracy and prognostic value of MPO in patients...

journal_title：Clinical chemistry

authors： Reichlin T,Socrates T,Egli P,Potocki M,Breidthardt T,Arenja N,Meissner J,Noveanu M,Reiter M,Twerenbold R,Schaub N,Buser A,Mueller C

更新日期：2010-06-01 00:00:00

abstract：BACKGROUND:The antiphospholipid syndrome (APS) is an important cause of acquired thromboembolic complications and pregnancy morbidity. Its diagnosis is based on clinical and laboratory criteria, defined by strict guidelines. The original clinical and laboratory criteria for the identification of APS patients were publi...

journal_title：Clinical chemistry

authors： Devreese K,Hoylaerts MF

更新日期：2010-06-01 00:00:00

abstract：BACKGROUND:Reduced renal function is an established risk factor for cardiovascular events. We compared 3 measures of renal function--serum cystatin C, serum creatinine, and calculated creatinine clearance--as predictors of subsequent cardiovascular events in a community-based population of elderly individuals. METHODS...

journal_title：Clinical chemistry

authors： Beilby J,Divitini ML,Knuiman MW,Rossi E,Hung J

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:Standardization of hemoglobin (Hb)A(1c) measurements is a process of considerable interest for quality assurance in diabetes management. To contribute to continuous quality improvement and fulfillment of the requirements for reference measurement procedures according to the standards of the International Org...

journal_title：Clinical chemistry

authors： Kaiser P,Akerboom T,Ohlendorf R,Reinauer H

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:Gradient gel electrophoresis (GGE) and nuclear magnetic resonance (NMR) spectroscopy are both widely accepted methods for measuring LDL and HDL particle size. However, whether or not GGE- or NMR-measured LDL or HDL particle size predicts coronary heart disease (CHD) risk to a similar extent is currently unkn...

journal_title：Clinical chemistry

authors： Arsenault BJ,Lemieux I,Després JP,Wareham NJ,Stroes ES,Kastelein JJ,Khaw KT,Boekholdt SM

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:A reduced response to aspirin and clopidogrel predicts ischemic events, but reliable tests are needed to identify low responders. We compared 3 platelet-function tests during long-term dual treatment with aspirin and clopidogrel. METHODS:Patients who underwent a percutaneous coronary intervention and were r...

journal_title：Clinical chemistry

authors： Madsen EH,Saw J,Kristensen SR,Schmidt EB,Pittendreigh C,Maurer-Spurej E

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:Lectin-like oxidized LDL receptor 1 (LOX-1) is implicated in atherothrombotic diseases. Activation of LOX-1 in humans can be evaluated by use of the LOX index, obtained by multiplying the circulating concentration of LOX-1 ligands containing apolipoprotein B (LAB) times that of the soluble form of LOX-1 (sLO...

journal_title：Clinical chemistry

authors： Inoue N,Okamura T,Kokubo Y,Fujita Y,Sato Y,Nakanishi M,Yanagida K,Kakino A,Iwamoto S,Watanabe M,Ogura S,Otsui K,Matsuda H,Uchida K,Yoshimoto R,Sawamura T

更新日期：2010-04-01 00:00:00

abstract：BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...

journal_title：Clinical chemistry

authors： Lindau-Shepard BA,Pass KA

更新日期：2010-03-01 00:00:00

abstract：BACKGROUND:The new Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation was developed to address the systematic underestimation of the glomerular filtration rate (GFR) by the Modification of Diet in Renal Disease (MDRD) Study equation in patients with a relatively well-preserved kidney function. The per...

journal_title：Clinical chemistry

authors： White CA,Akbari A,Doucette S,Fergusson D,Knoll GA

更新日期：2010-03-01 00:00:00

abstract：BACKGROUND:C-reactive protein (CRP) increases in response to inflammation and is purported to be a risk factor for atherogenesis. We recently demonstrated that a scavenger receptor, lectin-like oxidized LDL receptor (LOX-1), is a receptor for CRP. In light of the overlapping ligand spectrum of scavenger receptors such ...

journal_title：Clinical chemistry

authors： Fujita Y,Kakino A,Harada-Shiba M,Sato Y,Otsui K,Yoshimoto R,Sawamura T

更新日期：2010-03-01 00:00:00

abstract：BACKGROUND:Immunoassays specific for 1-84 parathyroid hormone (PTH) reportedly reflect the bioactivity of PTH; however, PTH immunoassays can be susceptible to interference by cross-reacting PTH fragments. In addition, these assays currently lack standardization. A methodology using immunocapture purification with liqui...

journal_title：Clinical chemistry

authors： Kumar V,Barnidge DR,Chen LS,Twentyman JM,Cradic KW,Grebe SK,Singh RJ

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND:Multiple reaction monitoring mass spectrometry (MRM-MS) of peptides with stable isotope-labeled internal standards (SISs) is increasingly being used to develop quantitative assays for proteins in complex biological matrices. These assays can be highly precise and quantitative, but the frequent occurrence of ...

journal_title：Clinical chemistry

authors： Abbatiello SE,Mani DR,Keshishian H,Carr SA

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND:Serial measurements of neurohormones have been shown to improve prognostication in the setting of acute heart failure (HF) or chronic HF without therapeutic intervention. We investigated the prognostic role of serial measurements of emerging neurohormones and BNP in a cohort of chronic HF patients undergoing...

journal_title：Clinical chemistry

authors： Neuhold S,Huelsmann M,Strunk G,Struck J,Adlbrecht C,Gouya G,Elhenicky M,Pacher R

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Methylmalonic acid (MMA) in plasma or serum is widely used for assessment of vitamin B(12) status. However, data are sparse regarding factors, besides renal function, that may influence MMA concentrations. We searched for important determinants of plasma MMA in the general population. METHODS:In 6946 middle...

journal_title：Clinical chemistry

authors： Vogiatzoglou A,Oulhaj A,Smith AD,Nurk E,Drevon CA,Ueland PM,Vollset SE,Tell GS,Refsum H

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS:We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLi...

journal_title：Clinical chemistry

authors： Wenzel JJ,Walch H,Bollwein M,Niller HH,Ankenbauer W,Mauritz R,Höltke HJ,Zepeda HM,Wolf H,Jilg W,Reischl U

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:Although cardiac troponin (cTn) is a cornerstone marker in the assessment and management of patients with acute coronary syndrome (ACS) and heart failure (HF), cTn is not diagnostically specific for any single myocardial disease process. This narrative review discusses increases in cTn that result from acute...

journal_title：Clinical chemistry

authors： Kelley WE,Januzzi JL,Christenson RH

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. ME...

journal_title：Clinical chemistry

authors： Seegmiller JC,Sviridov D,Larson TS,Borland TM,Hortin GL,Lieske JC

更新日期：2009-11-01 00:00:00

abstract：BACKGROUND:The next-generation, high-sensitivity cardiac troponin assays can measure quantifiable concentrations of cTn in a majority of individuals, but there are few studies assessing these assays for risk stratification. The present study was undertaken to determine if a research hs-cTnI assay can be useful for pred...

journal_title：Clinical chemistry

authors： Kavsak PA,Wang X,Ko DT,MacRae AR,Jaffe AS

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Vascular smooth muscle cells (SMCs) migrate from the arterial media to the intima in the progression of atherosclerosis, and dysfunction of SMCs leads to enhanced atherogenesis. A soluble form of the LDL receptor relative with 11 ligand-binding repeats (sLR11) is produced by the intimal SMCs, and the circula...

journal_title：Clinical chemistry

authors： Matsuo M,Ebinuma H,Fukamachi I,Jiang M,Bujo H,Saito Y

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Adequate detection of designer steroids in the urine of athletes is still a challenge in doping control analysis and requires knowledge of steroid metabolism. In this study we investigated whether uPA(+/+)-SCID mice carrying functional primary human hepatocytes in their liver would provide a suitable alterna...

journal_title：Clinical chemistry

authors： Lootens L,Meuleman P,Pozo OJ,Van Eenoo P,Leroux-Roels G,Delbeke FT

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Tamoxifen is a standard endocrine therapy for the prevention and treatment of steroid hormone receptor-positive breast cancer. CONTENT:Tamoxifen requires enzymatic activation by cytochrome P450 (CYP) enzymes for the formation of active metabolites 4-hydroxytamoxifen and endoxifen. As compared with the paren...

journal_title：Clinical chemistry

authors： Brauch H,Mürdter TE,Eichelbaum M,Schwab M

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Altered germline expression of genes may represent a powerful marker of genetic or epigenetic predisposition to cancer or other diseases. METHODS:We developed and validated a method of nonfluorescent primer extension that uses a single dideoxynucleotide and denaturing HPLC (DHPLC) to analyze the relative al...

journal_title：Clinical chemistry

authors： Aceto GM,De Lellis L,Catalano T,Veschi S,Radice P,Di Iorio A,Mariani-Costantini R,Cama A,Curia MC

更新日期：2009-09-01 00:00:00

abstract：BACKGROUND:Formalin-fixed paraffin-embedded (FFPE) tumor material represents a valuable resource for the analysis of RNA-based biomarkers, both in research laboratories and in routine clinical testing. A robust and automated RNA-extraction method with a high sample throughput is required. METHODS:We evaluated extracti...

journal_title：Clinical chemistry

authors： Bohmann K,Hennig G,Rogel U,Poremba C,Mueller BM,Fritz P,Stoerkel S,Schaefer KL

更新日期：2009-09-01 00:00:00

abstract：BACKGROUND:The prognostic value of changes in paraprotein markers after stem cell transplantation is unknown. We evaluated disease response using serum immunofixation (s-IFIX), total kappa and lambda ratio (KLR), and free light chain (FLC) ratio in myeloma patients who underwent autologous or autologous plus allogeneic...

journal_title：Clinical chemistry

authors： Giarin MM,Giaccone L,Sorasio R,Sfiligoi C,Amoroso B,Cavallo F,Cipriani A,Palumbo A,Boccadoro M

更新日期：2009-08-01 00:00:00

abstract：BACKGROUND:PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response in bone marrow (BM) and peripheral blood (PB). PHOX2B has been identified as a sensitive and specific MRD marker; however, its expression varies between tumors....

journal_title：Clinical chemistry

authors： Stutterheim J,Gerritsen A,Zappeij-Kannegieter L,Yalcin B,Dee R,van Noesel MM,Berthold F,Versteeg R,Caron HN,van der Schoot CE,Tytgat GA

更新日期：2009-07-01 00:00:00

abstract：BACKGROUND:In patient and population samples, generation of analytical results that are comparable and independent of the measurement system, time, and location is essential for the utility of laboratory information supplied in healthcare. Obtaining analytical measurement results with such characteristics is the aim of...

journal_title：Clinical chemistry

authors： Vesper HW,Thienpont LM

更新日期：2009-06-01 00:00:00

abstract：BACKGROUND:Measurement of plasma renin is important for the clinical assessment of hypertensive patients. The most common methods for measuring plasma renin are the plasma renin activity (PRA) assay and the renin immunoassay. The clinical application of renin inhibitor therapy has thrown into focus the differences in i...

journal_title：Clinical chemistry

authors： Campbell DJ,Nussberger J,Stowasser M,Danser AH,Morganti A,Frandsen E,Ménard J

更新日期：2009-05-01 00:00:00

abstract：BACKGROUND:A previously developed monoclonal/polyclonal ELISA (Mono/Poly) to detect plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in breast, prostate, and other cancers. Here we describe the clinical validation of a new dual monoclonal (Dual Mono) assay. We compared both assays ...

journal_title：Clinical chemistry

authors： Anborgh PH,Wilson SM,Tuck AB,Winquist E,Schmidt N,Hart R,Kon S,Maeda M,Uede T,Stitt LW,Chambers AF

更新日期：2009-05-01 00:00:00

abstract：BACKGROUND:National Cholesterol Education Program (NCEP) guidelines recommend development of direct assays for LDL cholesterol (LDL-C) measurement, but it is unclear how these assays compare with Friedewald calculation in predicting cardiovascular disease (CVD). METHODS:In a study of 27 331 healthy women with triglyce...

journal_title：Clinical chemistry

authors： Mora S,Rifai N,Buring JE,Ridker PM

更新日期：2009-05-01 00:00:00

abstract：BACKGROUND:Patients differ in responses to warfarin, which is commonly prescribed to treat thromboembolic events. Genetic variations in the cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9), vitamin K epoxide reductase complex, subunit 1 (VKORC1), and gamma-glutamyl carboxylase (GGCX) genes have been shown...

journal_title：Clinical chemistry

authors： Rai AJ,Udar N,Saad R,Fleisher M

更新日期：2009-04-01 00:00:00

abstract：BACKGROUND:For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). ...

journal_title：Clinical chemistry

authors： Voelkerding KV,Dames SA,Durtschi JD

更新日期：2009-04-01 00:00:00

abstract：BACKGROUND:The term "transrenal DNA" was coined in 2000 to signify that DNA in urine may come from the passage of plasma DNA through the kidney barrier. Although DNA in the urine has the potential to provide a completely noninvasive source of nucleic acids for molecular diagnosis, its existence remains controversial. ...

journal_title：Clinical chemistry

authors： Hung EC,Shing TK,Chim SS,Yeung PC,Chan RW,Chik KW,Lee V,Tsui NB,Li CK,Wong CS,Chiu RW,Lo YM

更新日期：2009-04-01 00:00:00

abstract：BACKGROUND:The determination of cellular beta-galactocerebrosidase activity is an established procedure to diagnose Krabbe disease and monitor the efficacy of gene/stem cell-based therapeutic approaches aimed at restoring defective enzymatic activity in patients or disease models. Current biochemical assays for beta-ga...

journal_title：Clinical chemistry

authors： Martino S,Tiribuzi R,Tortori A,Conti D,Visigalli I,Lattanzi A,Biffi A,Gritti A,Orlacchio A

更新日期：2009-03-01 00:00:00

abstract：BACKGROUND:Adiponectin is an adipocyte-derived hormone circulating in different multimer complexes. The high-molecular-weight (HMW) complex is likely the active form of this protein and has been recognized as a risk marker for type 2 diabetes and coronary artery disease (CAD). Because quantification of HMW adiponectin ...

journal_title：Clinical chemistry

authors： Liu D,Schuster T,Baumann M,Roos M,Sollinger D,Lutz J,Heemann U,von Eynatten M

更新日期：2009-03-01 00:00:00

abstract：BACKGROUND:High-sensitivity C-reactive protein (hsCRP) testing is relatively inexpensive and has been shown to predict the risk of cardiovascular disease (CVD) and diabetes in multiple patient groups, including those treated with statin therapy. JUPITER (Justification for the Use of Statins in Primary Prevention: An In...

journal_title：Clinical chemistry

authors： Mora S,Musunuru K,Blumenthal RS

更新日期：2009-02-01 00:00:00