Abstract:
BACKGROUND:Vascular smooth muscle cells (SMCs) migrate from the arterial media to the intima in the progression of atherosclerosis, and dysfunction of SMCs leads to enhanced atherogenesis. A soluble form of the LDL receptor relative with 11 ligand-binding repeats (sLR11) is produced by the intimal SMCs, and the circulating concentrations of sLR11 likely reflect the pathophysiological condition of intimal SMCs. Furthermore, polymorphism of the LR11 gene has been found to be related to the onset of Alzheimer disease. This study describes the development of a sandwich immunoassay for quantifying sLR11 in human serum and cerebrospinal fluid. METHODS:We used synthetic peptides or DNA immunization to produce monoclonal antibodies (MAbs) A2-2-3, M3, and R14 against different epitopes of LR11. RESULTS:sLR11 was immunologically identified as a 250-kDa protein in human serum and cerebrospinal fluid by SDS-PAGE separation, and was purified from serum by use of a receptor-associated protein and MAb M3. An immunoassay for quantification of sLR11 with a working range of 0.25-4.0 microg/L was developed using the combination of MAbs M3 and R14. Treatment of serum with 5.25% n-nonanoyl-N-methyl-d-glucamine reduced the matrix effects of serum on the absorbance detection in the ELISA system. The linear dynamic range of the ELISA spanned the variation of circulating sLR11 concentrations in individuals with atherosclerosis. CONCLUSIONS:A sandwich ELISA was established for quantifying sLR11 in serum and cerebrospinal fluid. This technique provides a novel means for assessing the pathophysiology of atherosclerosis, and possibly neurodegenerative diseases.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Matsuo M,Ebinuma H,Fukamachi I,Jiang M,Bujo H,Saito Ydoi
10.1373/clinchem.2009.127027subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1801-8issue
10eissn
0009-9147issn
1530-8561pii
clinchem.2009.127027journal_volume
55pub_type
杂志文章abstract::Diabetic patients in poor glycemic control show increased glycation of total plasma proteins, but little is yet known about the relative extents to which the various individual proteins are glycated. Thus, we studied the non-enzymic glycation of several major plasma proteins and plasma protein fractions in normal and ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract:BACKGROUND:Quantification of 5-hydroxyindole-3-acetic acid (5-HIAA) in urine is useful in diagnosing and monitoring of patients with carcinoid tumors and in the study of serotonin (5-hydroxytryptamine) metabolism in various disorders. We describe an automated method that incorporates on-line solid-phase extraction (SPE...
journal_title:Clinical chemistry
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doi:10.1373/clinchem.2005.050062
更新日期:2005-09-01 00:00:00
abstract::This simple, accurate, and reproducible colorimetric method for determining the activity of angiotensin-I converting enzyme is based upon colorimetry of the quinoneimine dye produced from the substrate p-hydroxyhippuryl-L-histidyl-L-leucine by action of this enzyme through the following series of reactions. The enzyme...
journal_title:Clinical chemistry
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更新日期:1981-11-01 00:00:00
abstract:BACKGROUND:Recent use of Bacillus anthracis spores as a bioweapon has highlighted the need for a continuous monitoring system. Current monitoring systems rely on antibody-derived probes, which are not hardy enough to withstand long-term use under extreme conditions. We describe new, phage-derived probes that can be use...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.038018
更新日期:2004-10-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1992-04-01 00:00:00
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更新日期:2009-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::We measured certain enzyme activities (aldolase, aspartate aminotransferase, creatine kinase, and lactate dehydrogenase) and inflammation markers (alpha 1-antitrypsin, C-reactive protein, fibrinogen, and leukocytes) each day for four days in plasma of patients with severe head injury. The univariate prognostic efficie...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1989-07-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:2000-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:10.1373/clinchem.2005.062604
更新日期:2007-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
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更新日期:2009-04-01 00:00:00
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更新日期:2019-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1977-01-01 00:00:00
abstract::Using high-performance liquid chromatography and gas chromatography, we reevaluated the 24-h influence of a serotonin- and dopamine-rich diet on platelet serotonin and serotonin, 5-hydroxyindoleacetic acid (5-HIAA), and major catecholamine metabolites in the urine of 15 healthy adults. Although there were significant ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::A high (2.0 mol/L) NaCl concentration apparently suppresses the secondary-site binding of bilirubin by human serum albumin. Thus if an excess of bilirubin is added to human serum albumin or to neonatal serum in buffer containing 0.1 mol of tris(hydroxymethyl)aminomethane and 2.0 mol of NaCl per liter (pH 7.5), and the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Two major components of rRNA (18S and 28S rRNA) were separated by electrophoresis in injection-molded acrylic chips with a microchannel 100 microm in width, 40 microm in depth, and with 1 cm of separation distance. Microchannels were filled with 4 g/L hydroxypropylmethylcellulose as sieving polymer and 5 mg/L ethidium...
journal_title:Clinical chemistry
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doi:
更新日期:1998-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1995-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1981-07-01 00:00:00
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pub_type: 临床试验,杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Pairs of values for serum triiodothyronine and serum triiodothyronine binding capacity were represented as maps of points for euthyroid populations (including subjects who were pregnant or were receiving estrogens) in the age groups 18-50, 51-64, 65-79, and greater than 80 years (504 subjects) and thyrotoxic populatio...
journal_title:Clinical chemistry
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doi:
更新日期:1978-10-01 00:00:00
abstract:BACKGROUND:Some of the genetic variants of hemoglobin (Hb) and their chemically modified species are known to affect the measurement of Hb A(1c). The purpose of this study was to characterize Hb species in the blood sample of a 74-year-old German male with an exceptionally low Hb A(1c) value. METHODS:Hemolysates from ...
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更新日期:2003-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1985-07-01 00:00:00
abstract::We describe a simple, highly reproducible kinetic technique for precisely measuring temperature in spectrophotometric systems having reaction cells that are inaccessible to conventional temperature probes. The method is based on the temperature dependence of pseudo-first-order rate constants for the acid-catalyzed hyd...
journal_title:Clinical chemistry
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doi:
更新日期:1981-05-01 00:00:00
abstract::I describe a simple, single-tube batch fractionation procedure for separating MM and MB isoenzymes of creatine kinase on a macroporous strong anion exchanger (AG MP-1, Bio-Rad Laboratories). The isoenzymes can be separated in less than 3 min, with a resulting dilution of the serum with no more than an equal volume of ...
journal_title:Clinical chemistry
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doi:
更新日期:1976-01-01 00:00:00
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更新日期:2011-02-01 00:00:00