解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::To identify genes important for taste receptor cell function, we analyzed the sequences and expression patterns of clones isolated from a mouse taste receptor cell-enriched cDNA library. Here, we report the analyses of two novel genes, Gpr113 and Trcg1. Gpr113 encodes a G-protein-coupled receptor belonging to family 2...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.12.005
更新日期:2005-04-01 00:00:00
abstract::Genes playing essential roles in iron homeostasis have yet to be identified. We report the discovery of a strong candidate gene affecting iron homeostasis in two allelic anemia mouse mutants: hea (hereditary erythroblastic anemia) and fsn (flaky skin). To clone this novel gene positionally, we established a large back...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.11.008
更新日期:2005-03-01 00:00:00
abstract::Friedreich ataxia results from frataxin insufficiency caused by repeat expansion in intron 1 of the frataxin gene. Since the coding sequence is unchanged, the potential exists to ameliorate symptoms by increasing frataxin promoter activity. We therefore defined the minimal frataxin promoter in humans. Despite the fact...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.10.013
更新日期:2005-02-01 00:00:00
abstract::Fluorescence in situ hybridization mapping of fully integrated human BAC clones to primate chromosomes, combined with precise breakpoint localization by PCR analysis of flow-sorted chromosomes, was used to analyze the evolutionary rearrangements of the human 3q21.3-syntenic region in orangutan, siamang gibbon, and sil...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.10.007
更新日期:2005-01-01 00:00:00
abstract::We have analyzed the organization of the chicken alpha-globin gene domain using DNA miniarrays and have found two novel chromatin loop attachment regions. We have found a 40-kb loop domain that includes all the alpha-globin genes in cells of erythroid origin. One of the domain borders colocalizes almost exactly with a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.09.008
更新日期:2005-01-01 00:00:00
abstract::Central to reconstruction of cis-regulatory networks is identification and classification of naturally occurring transcription factor-binding sites according to the genes that they control. We have examined salient characteristics of 9-mers that occur in various orders and combinations in the proximal promoters of hum...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.08.013
更新日期:2004-12-01 00:00:00
abstract::This study has investigated the transcriptional regulation of the Emr1 gene in murine macrophages and defined an enhancer element within the proximal promoter that is necessary for Emr1 expression in myeloid cells. This element consists of an extended purine-rich sequence (PuRS) of 83 consecutive purine residues conta...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.08.016
更新日期:2004-12-01 00:00:00
abstract::Duplicated segments of genomic DNA can catalyze both gene evolution and chromosome evolution. Here we describe a rodent-specific duplication involving the Uqcrb gene, a cis-regulatory element for the Gdf6 gene, and a chromosomal rearrangement. Comparisons of Gdf6 sequences from several placental mammals and platypus r...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.07.009
更新日期:2004-11-01 00:00:00
abstract::A comparison study of short SAGE versus GeneChip and long SAGE was conducted to determine if data were interchangeable between the techniques. Although SAGE and Affymetrix chip expression levels showed a significant correlation using the set of genes for which there was reliable and unambiguous mapping from tag-to-gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.06.014
更新日期:2004-10-01 00:00:00
abstract::Inducible costimulatory receptor (ICOS) is one recently identified member of the CD28 family of costimulatory molecules. Evidence suggests ICOS functions as a critical immune regulator and, to evaluate these effects, we employed the canine model system that has been used to develop strategies currently in clinical use...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.06.009
更新日期:2004-10-01 00:00:00
abstract::The gene mutated in chorea-acanthocytosis (CHAC; approved gene symbol VPS13A) encodes chorein, a protein similar to yeast Vps13p. We detected several similar putative human proteins by BLAST analysis of chorein. We characterized the structure of three new genes encoding these CHAC-similar proteins, located on chromoso...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.04.012
更新日期:2004-09-01 00:00:00
abstract::We report a comparative map of canine chromosome 1 (CFA1) incorporating single nucleotide polymorphisms (SNPs) and insertion/deletion (indel) polymorphisms, developed by using cross-species primers, radiation hybrid analysis, and pool-and-sequence identification of genetic variations. Fifty-five genes were chosen with...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.04.001
更新日期:2004-08-01 00:00:00
abstract::A cluster of genes coding for proteins of the extracellular matrix (ECM) containing sequence motifs essential for integrin-receptor interactions is located on HSA4q21 and on BTA6, within the critical region of a quantitative trait locus (QTL) affecting milk protein production. Genes within this cluster are involved in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.03.005
更新日期:2004-08-01 00:00:00
abstract::The EF-hand superfamily of calcium binding proteins includes the S100, calcium binding protein, and troponin subfamilies. This study represents a genome, structure, and expression analysis of the S100 protein family, in mouse, human, and rat. We confirm the high level of conservation between mammalian sequences but sh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.02.002
更新日期:2004-07-01 00:00:00
abstract::Mutations in genes encoding polycystin-1 (PC1) and polycystin-2 cause autosomal dominant polycystic kidney disease. The polycystin protein family is composed of Ca2+-permeable pore-forming subunits and receptor-like integral membrane proteins. Here we describe a novel member of the polycystin-1-like subfamily, polycys...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.02.008
更新日期:2004-07-01 00:00:00
abstract::The cytotoxic activity of mouse natural killer cells is regulated in part through cell surface molecules belonging to the Ly49 multigene family. In mice, the genomic sequence of the Ly49 gene cluster has been examined in detail and this analysis provided a model of the expansion of this multigene family. In the presen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.01.010
更新日期:2004-07-01 00:00:00
abstract::To identify novel genes regulating the biologic response to lipopolysaccharide (LPS), we used a combination of quantitative trait locus (QTL) analysis and microarray-based gene expression studies of C57BL/6J x DBA/2J(BXD) F2 and recombinant inbred (RI) mice. A QTL affecting pulmonary TNF-alpha production was identifie...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.12.008
更新日期:2004-06-01 00:00:00
abstract::The hypoxanthine phosphoribosyltransferase (Hprt) locus has been shown to have minimal influence on transgene expression when used as a surrogate site in the mouse genome. We have developed a method to transfer bacterial artificial chromosomes (BACs) as a single copy into the partially deleted Hprt locus of embryonic ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.12.015
更新日期:2004-06-01 00:00:00
abstract::We present evidence of a potentially serious source of error intrinsic to all spotted cDNA microarrays that use IMAGE clones of expressed sequence tags (ESTs). We found that a high proportion of these EST sequences contain 5'-end poly(dT) sequences that are remnants from the oligo(dT)-primed reverse transcription of p...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.12.010
更新日期:2004-06-01 00:00:00
abstract::Odorant receptors (ORs) and vomeronasal receptors (V1Rs and V2Rs) are large superfamilies of chemosensory receptors. As an extension of previous research using the 2001 Celera mouse genome assembly, we analyzed OR and V1R genes in the 2002 public mouse genome assembly. We identified 1403 OR genes (1068 potentially int...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.10.009
更新日期:2004-05-01 00:00:00
abstract::Members of the apolipoprotein gene cluster (APOA1/C3/A4/A5) on human chromosome 11q23 play an important role in lipid metabolism. Polymorphisms in both APOA5 and APOC3 are strongly associated with plasma triglyceride concentrations. The close genomic locations of these two genes as well as their functional similarity ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.11.016
更新日期:2004-05-01 00:00:00
abstract::One of the major challenges in genome research is the identification of the complete set of genes in a genome. Alignments of expressed sequences (RNA and EST) with genomic sequences have been used to characterize genes. However, the number of alignments far exceeds the likely number of genes in a genome, suggesting th...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.07.003
更新日期:2004-04-01 00:00:00
abstract::Recently, we identified multiple unique sequences in the 21q22.3 region and predicted them to be a cluster of genes encoding hair-specific keratin-associated proteins (KAPs). Detailed computer-aided analysis of these clustered genes revealed that the cluster spans over 165 kb and consists of 21 KAP-related sequences i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.09.024
更新日期:2004-04-01 00:00:00
abstract::Several mammalian sialidases have been cloned so far and here we describe the identification and expression of a new member of the human sialidase gene family. The NEU4 gene, identified by searching sequence databases for entries showing homologies to the human cytosolic sialidase NEU2, maps in 2q37 and encodes a 484-...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.08.019
更新日期:2004-03-01 00:00:00
abstract::The canine species, including wolf and jackal, have four digits on the hind limb. It was thought that an extra first digit on the hind limb, named dewclaw, was a hereditary defect. For genetically related canine pedigrees with 73 members with dewclaws, we carried out a genome-wide scan for linkage with microsatellites...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00234-9
更新日期:2004-02-01 00:00:00
abstract::TaqMAMA combines the quantitative strengths of TaqMan with the allele-specific PCR of MAMA. In this article we develop TaqMAMA as a technique for screening human DNA samples for known genetic polymorphisms. In the first set of experiments, plasmids that model all types of genetic polymorphisms were used to understand ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.08.005
更新日期:2004-02-01 00:00:00
abstract::Bacterial artificial chromosomes (BACs) provide a well-characterized resource for studying the functional organization of genes and other large chromosomal domains. To facilitate functional studies in cell cultures, we have developed a simple approach for generating stable cell lines with variable copy numbers of any ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00205-2
更新日期:2004-01-01 00:00:00
abstract::Histamine N-methyltransferase (HNMT), a cytosolic histamine-metabolizing enzyme, is the only known product of the 50-kb human HNMT. Here, a detailed investigation of HNMT products revealed the existence of a new brain mRNA product of HNMT. This species, named HNMT-Short (HNMT-S), encodes a 126-amino-acid protein. Nort...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00236-2
更新日期:2004-01-01 00:00:00
abstract::Alternative splicing is an important cellular mechanism that increases the diversity of gene products. The number of alternatively spliced genes reported so far in plants is much smaller than that in mammals, but is increasing as a result of the explosive growth of available EST and genomic sequences. We have searched...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00204-0
更新日期:2003-12-01 00:00:00
abstract::G-protein-coupled receptors (GPCRs) are important mediators of signal transduction, and mutations in GPCR-encoding genes can lead to disease states. Here we describe a null mutation in an orphan GPCR-encoding gene that is predicted to inactivate completely the encoded receptor. The TA(3) receptor is a putative member ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00173-3
更新日期:2003-11-01 00:00:00
abstract::It is hypothesized that autosomal retroposons compensate for the loss of their inactivated essential X-chromosome progenitors during spermatogenesis. Here we test this Retroposon Compensatory Mechanism (RCM) hypothesis using the Zfy gene family. The mouse autosomal retroposon Zfa is expressed in testes at the same dev...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00155-1
更新日期:2003-09-01 00:00:00
abstract::We report a comprehensive comparative analysis of human and mouse olfactory receptor (OR) genes encoding OR37 subtypes to determine the repertoire, chromosomal organization, and relatedness of these genes. Two OR37 clusters were found in both mouse (chromosome 4) and human (chromosome 9); with five genes in cluster I ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00116-2
更新日期:2003-09-01 00:00:00
abstract::A dense gene-based SNP map was constructed across a 360-kb region containing the interleukin-1 gene cluster (IL1A, IL1B, and IL1RN), focusing on IL1RN. In total, 95 polymorphisms were confirmed or identified primarily by direct sequencing. Polymorphisms were precisely mapped to completed BAC and genomic sequences span...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00123-x
更新日期:2003-08-01 00:00:00
abstract::TM4SF11 is only 102 kb from the chemokine gene cluster composed of SCYA22, SCYD1, and SCYA17 on chromosome 16q13. CKLF maps on chromosome 16q22. CKLFs have some characteristics associated with the CCL22/MDC, CX3CL1/fractalkine, CCL17/TARC, and TM4SF proteins. Bioinformatics based on CKLF2 cDNA and protein sequences in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00095-8
更新日期:2003-06-01 00:00:00
abstract::On the basis of previous observations in chromosomes 21 and 22, we hypothesize that there is a tissue-specific organization of cardiovascular gene transcripts in the human genome. To examine the distribution of heart-derived transcripts, we assigned a nonredundant set of 4628 fetal and 3574 adult known and uncharacter...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00008-9
更新日期:2003-05-01 00:00:00
abstract::Cataract Tohoku (Cat(Tohm)) is a dominant cataract mutation that leads to severe degeneration of lens fiber cells. Linkage analysis showed that the Cat(Tohm) mutation is located on mouse chromosome 10, close to the gene for aquaporin-0 (Aqp0), which encodes a membrane protein that is expressed specifically in lens fib...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00029-6
更新日期:2003-04-01 00:00:00
abstract::Loss of heterozygosity (LOH) of chromosomal regions is crucial in tumor progression. In this study we assessed the potential of the Affymetrix GeneChip HuSNP mapping assay for detecting genome-wide LOH in prostate tumors. We analyzed two human prostate cell lines, P69SV40Tag (P69) and its tumorigenic subline, M12, and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00020-x
更新日期:2003-03-01 00:00:00
abstract::RIMs are synaptic proteins that are essential for normal neurotransmitter release. We now show that while invertebrates contain only a single RIM gene, vertebrates contain four: two large genes encoding RIM1alpha (0.50 Mb) or RIM2alpha, 2beta, and 2gamma (0.50-0.75 Mb) and two smaller genes encoding RIM3gamma (14 kb) ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(02)00024-1
更新日期:2003-02-01 00:00:00
abstract::We have reported that a novel c-Myc binding protein, AMY-1, stimulated the transcription activity of c-Myc and was translocated from the cytoplasm to the nucleus in a c-Myc-dependent manner. AMY-1 works as an inducer of human K562 cell differentiation upon induction of AraC. To characterize the expression or functiona...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00006-5
更新日期:2003-02-01 00:00:00
abstract::We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3' end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5' end b...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(02)00009-5
更新日期:2003-01-01 00:00:00