Abstract:
:We have analyzed the organization of the chicken alpha-globin gene domain using DNA miniarrays and have found two novel chromatin loop attachment regions. We have found a 40-kb loop domain that includes all the alpha-globin genes in cells of erythroid origin. One of the domain borders colocalizes almost exactly with a strong MAR element and with a block of enhancer-blocking elements found earlier at the upstream end of the alpha-globin gene domain. The domain structure was found to be different in a lymphoid cell line DT40. We propose to use the technique of DNA arrays to map the nuclear matrix attachment sites that define the borders of chromosome loop domains. The technique of DNA arrays permits a large number of DNA sequences to be immobilized on a glass or nylon matrix. This may prove useful for mapping chromatin loop positions within the human genome by using a pool of chromatin loop attachment regions as a probe in a hybridization with a DNA chip containing a specific DNA region.
journal_name
Genomicsjournal_title
Genomicsauthors
Ioudinkova E,Petrov A,Razin SV,Vassetzky YSdoi
10.1016/j.ygeno.2004.09.008subject
Has Abstractpub_date
2005-01-01 00:00:00pages
143-51issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(04)00272-1journal_volume
85pub_type
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