Abstract:
:Environmental decline triggers a switch in reproductive strategy of Daphnia magna from asexual to sexual reproduction; however, the molecular basis of such environmental sex determination remains largely unknown. In insects most closely related to branchiopod crustaceans, orthologs of the Drosophila transformer gene such as dipteran transformer (tra), honeybee feminizer (fem) and complementary sex determiner (csd) function as sex determining factors. Therefore, we cloned a D. magna transformer gene (dmagtra) and analyzed its expression. The predicted amino acid sequence has 380 amino acids including an arginine-serine-rich region, which is characteristic of insect orthologs of Tra. Residues 180 to 205 are highly conserved with known Tra orthologs. The domain organization of DmagTra is distinct from known Tra orthologs; moreover, dmagtra does not display any detectable sexual dimorphic differences in expression or splicing patterns. We infer from these results that dmagtra may not be responsible for sex determination in D. magna.
journal_name
Genomicsjournal_title
Genomicsauthors
Kato Y,Kobayashi K,Oda S,Tatarazako N,Watanabe H,Iguchi Tdoi
10.1016/j.ygeno.2009.12.005subject
Has Abstractpub_date
2010-03-01 00:00:00pages
160-5issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(09)00275-4journal_volume
95pub_type
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