Abstract:
:A gene family of Na+/H+ exchanger isoforms has been identified. Characterization of rabbit NHE3 suggests that it is the apical epithelial Na+/H+ exchanger isoform responsible for transepithelial, electroneutral Na+ absorption in intestinal and renal epithelial cells. We have previously isolated from a human kidney cortex library a partial human NHE3 cDNA, clone HKC-3. Using HKC-3 to probe human/rodent somatic cell hybrid mapping panels, the human NHE3 gene was physically mapped to the distal portion of chromosome 5p15.3. Southern analysis of EcoRI-digested human genomic DNAs of CEPH pedigrees probed with HKC-3 detected three polymorphic sites containing a total of nine alleles that segregate in a Mendelian fashion. The observed heterozygosity for the NHE3 locus in unrelated individuals was 71%. Linkage analysis between NHE3 and other markers known to map at 5p15 confirmed the localization of NHE3 to chromosome 5p15.3, making NHE3 the most telomeric gene identified on the short arm of chromosome 5.
journal_name
Genomicsjournal_title
Genomicsauthors
Brant SR,Bernstein M,Wasmuth JJ,Taylor EW,McPherson JD,Li X,Walker S,Pouyssegur J,Donowitz M,Tse CMdoi
10.1006/geno.1993.1122subject
Has Abstract,Author List Incompletepub_date
1993-03-01 00:00:00pages
668-72issue
3eissn
0888-7543issn
1089-8646pii
S0888754383711225journal_volume
15pub_type
杂志文章相关文献
GENOMICS文献大全abstract::We describe the construction of a high-resolution radiation hybrid (RH) map of the domestic cat genome, which includes 2662 markers, translating to an estimated average intermarker distance of 939 kilobases (kb). Targeted marker selection utilized the recent feline 1.9x genome assembly, concentrating on regions of low...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.09.010
更新日期:2009-04-01 00:00:00
abstract::We analyzed the whole genomes of cecum microbiomes of Ethiopian indigenous chickens from two distinct geographical zones: Afar (AF) district (Dulecha, 730 m above sea level) and Amhara (AM) district (Menz Gera Midir, 3300 m). Through metagenomic analysis we found that microbial populations were mainly dominated by Bac...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.11.011
更新日期:2020-03-01 00:00:00
abstract::Small-scale changes in gene order and orientation are common in plant genomes, even across relatively short evolutionary distances. We investigated the association of retrotransposons in and near rice gene pairs with gene pair conservation, inversion, rearrangement, and deletion in sorghum, maize, and Brachypodium. Co...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2012.02.006
更新日期:2012-05-01 00:00:00
abstract::Fishes possess more genes than other vertebrates, possibly because of a genome duplication event during the evolution of the teleost (ray-finned) fish lineage. To further explore this idea, we cloned five genes encoding phosphoinositide-specific phospholipase C-delta (PLC-delta), designated respectively PoPLC-deltas, ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.07.012
更新日期:2008-11-01 00:00:00
abstract::To facilitate studies of the SRY gene, a 4741-bp portion of the sex-determining region of the human Y chromosome was sequenced and characterized. Two RNAs were found to hybridize to this genomic segment, one transcript deriving from SRY and the second cross-hybridizing to a pseudogene located 2.5 kb 5' of the SRY open...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1395
更新日期:1993-09-01 00:00:00
abstract::The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1256
更新日期:1994-05-01 00:00:00
abstract::The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome). An interspecific backcross of SB/Le and Mus spretus mice was used t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4478
更新日期:1997-01-15 00:00:00
abstract::The complete coding sequence for mouse tropoelastin was obtained from overlapping reverse transcriptase polymerase chain reaction (PCR) amplimers. These cDNA fragments were derived from mouse tropoelastin mRNA using PCR oligomers complementary to conserved domains within rat tropoelastin mRNA. A comparison of coding d...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1467
更新日期:1994-09-01 00:00:00
abstract::The region of the dystrophin gene containing introns 45-50 is characterized by a high rate of recombination events that give rise to large deletions causing dystrophinopathy. The nucleotide sequence of this intronic region has recently been released in GenBank. With the aim of further understanding the mechanism favor...
journal_title:Genomics
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::The identification of noncoding functional elements within vertebrate genomes, such as those that regulate gene expression, is a major challenge. Comparisons of orthologous sequences from multiple species are effective at detecting highly conserved regions and can reveal potential regulatory sequences. The GDF6 gene c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.05.003
更新日期:2005-09-01 00:00:00
abstract::The presence of an extra copy of human chromosome 21 (trisomy 21), especially region 21q22.2, causes many phenotypes in Down syndrome, including mental retardation. To study genes potentially responsible for some of these phenotypes, we cloned a human candidate gene (DYRK) from 21q22.2 and its murine counterpart (Dyrk...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0636
更新日期:1996-12-15 00:00:00
abstract::Spt3 is a Saccharomyces cerevisiae transcription factor that is required in vivo for the transcription of a number of RNA polymerase II-transcribed genes. We report the cloning of the gene encoding the human homologue of Spt3, SUPT3H, and its initial functional analysis. The human and yeast Spt3 homologues share an ov...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5500
更新日期:1998-10-01 00:00:00
abstract::Cardiomyopathies are severe degenerative disorders of the myocardium that lead to heart failure. During the last three decades bovine dilated cardiomyopathy (BDCMP) was observed worldwide in cattle of Holstein-Friesian origin. In the Swiss cattle population BDCMP affects Fleckvieh and Red Holstein breeds. The heart of...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.09.005
更新日期:2011-01-01 00:00:00
abstract::LRP1B is a novel candidate tumor suppressor gene that is inactivated by genetic and transcript alterations in nearly 50% of non-small-cell lung cancer cell lines. The gene-encoded protein is highly homologous to the gigantic lipoprotein receptor-related protein 1 (LRP1) that belongs to the family of low-density lipopr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6331
更新日期:2000-10-15 00:00:00
abstract::The genome sequence of Mycobacterium tuberculosis strain H37Rv is an important and valuable reference point in the study of M. tuberculosis phylogeny, molecular epidemiology, and drug-resistance mutations. However, it is becoming apparent that use of H37Rv as a sole reference genome in analysing clinical isolates pres...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2017.07.004
更新日期:2017-10-01 00:00:00
abstract::Thirteen moderately to highly informative microsatellite DNA polymorphisms based on (dC-dA)n.(dG-dT)n repeats were mapped to segments of human chromosome 5 using both linkage analysis and a panel of somatic cell hybrids which contained rearranged chromosomes. The markers were distributed throughout most of the length ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90077-r
更新日期:1991-11-01 00:00:00
abstract::We developed a model of influenza virus infection of neutrophils by inducing differentiation of the MPRO promyelocytic cell line. After 5 days of differentiation, about 20-30% of mature neutrophils could be detected. Only a fraction of neutrophils were infected by highly virulent influenza (HVI) virus, but were unable...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2012.11.008
更新日期:2013-02-01 00:00:00
abstract::Background Bioinformatics tools are of great significance and are used in different spheres of life sciences. There are wide variety of tools available to perform primary analysis of DNA and protein but most of them are available on different platforms and many remain undetected. Accessing these tools separately to pe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.08.010
更新日期:2020-11-01 00:00:00
abstract::An alarming increase in the human population necessitates doubling the world food production in the next few decades. Although a number of possible biotechnological measures are under consideration, central to these efforts is the development of transgenic crops to produce more food, and the traits with which plants c...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2017.07.007
更新日期:2017-10-01 00:00:00
abstract::More than 500 backcross progeny from four intersubspecific backcrosses were typed for six markers on distal mouse chromosome 16. Five of these represented genes that mapped within the Sod-1 to Ets-2 interval, which was shown previously to contain the weaver (wv) gene. The map order, including previously mapped referen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1336
更新日期:1993-08-01 00:00:00
abstract::Lymphocyte antigen-6 (LY-6) superfamily members are cysteine-rich, generally GPI-anchored cell surface proteins, which have definite or putative immune related roles. A cluster of five potential LY-6 superfamily members is located in the human and mouse major histocompatibility complex class III region. Comparative an...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2002.6794
更新日期:2002-07-01 00:00:00
abstract::Syndactyly in Holstein cattle is an autosomal recessive abnormality characterized by the fusion of the functional digits. This disorder has been previously mapped to the telomeric part of bovine chromosome 15. Here, we describe the fine-mapping of syndactyly in Holstein cattle to a 3.5-Mb critical interval using a com...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.05.007
更新日期:2006-11-01 00:00:00
abstract::The imprinted U2af1-rs1 gene that maps to mouse chromosome 11 is predominately expressed from the paternal allele. We examined the methylation of genomic sequences in and around the U2af1-rs1 locus to establish the extent of sequence modifications that accompanied the silencing of the maternal allele. The analysis of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0348
更新日期:1996-07-01 00:00:00
abstract::Next generation sequencing techniques produce enormous data but its analysis and visualization remains a big challenge. To address this, we have developed Genome Annotator Light(GAL), a Docker based package for genome analysis and data visualization. GAL integrated several existing tools and in-house programs inside a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.03.012
更新日期:2020-01-01 00:00:00
abstract::In line 4 transgenic mice, six to eight copies of a 50-kb lambda recombinant clone are arranged in a head-to-tail tandem array on chromosome 3. Embryos homozygous for the transgene become arrested in their development on Day 5 of gestation shortly after implantation. The insertion site was cloned using a small segment...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90216-f
更新日期:1992-05-01 00:00:00
abstract::A genetic map of nine loci defined by polymorphic DNA markers was created using a single cross of F344/N and LEW/N rats. The markers contained polymorphic simple sequence repeats identified in five genes, renin (Ren), cardiac troponin T (Tnnt3), synaptotagmin (Syt2), Na+,K(+)-ATPase catalytic subunit (Atp1a2), and the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1466
更新日期:1993-11-01 00:00:00
abstract::The localization of a gene responsible for a normal variant of the human electroencephalogram to the distal part of chromosome 20q is reported. A linkage analysis, including 17 families with 191 individuals, tested with 73 RFLPs and 22 blood and serological markers, was performed for the low-voltage electroencephalogr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90408-k
更新日期:1992-01-01 00:00:00
abstract::Receptors for cholcystokinin (CCK) can be pharmacologically classified into at least two distinct subtypes, CCKAR and CCKBR. In an effort to determine whether the CCKA and CCKB receptors may be associated with certain CNS or gastrointestinal diseases, we have localized and compared the human and mouse chromosomal loci...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80018-h
更新日期:1995-02-10 00:00:00
abstract::The v-ets oncogene family shares a conserved peptide motif called the ETS domain that mediates sequence-specific DNA binding. This motif is unique among transcription factor families. Using partially degenerate oligonucleotides from conserved regions of the ETS domain and the polymerase chain reaction, we isolated a n...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1169
更新日期:1994-03-15 00:00:00
abstract::Alternative splicing is an important cellular mechanism that increases the diversity of gene products. The number of alternatively spliced genes reported so far in plants is much smaller than that in mammals, but is increasing as a result of the explosive growth of available EST and genomic sequences. We have searched...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00204-0
更新日期:2003-12-01 00:00:00