Abstract:
:In line 4 transgenic mice, six to eight copies of a 50-kb lambda recombinant clone are arranged in a head-to-tail tandem array on chromosome 3. Embryos homozygous for the transgene become arrested in their development on Day 5 of gestation shortly after implantation. The insertion site was cloned using a small segment of the transgene as a probe. Comparison of the insertion site with the wildtype locus indicates that a 22-kb deletion of host DNA has occurred in line 4 mice. Restriction enzyme analyses showed that neither the tandem array nor the flanking chromosomal DNA had any detectable rearrangements. Sequencing of the junctions between host and foreign DNA, however, revealed insertions of small fragments of DNA of unknown origin as well as an insertion of a DNA segment derived from another region of the transgene. Therefore, disruption of an essential gene in the line 4 transgenic mouse may have been caused by the insertion of 300-400 kb of foreign DNA or a deletion of sequences in the host genome.
journal_name
Genomicsjournal_title
Genomicsauthors
Mark WH,Signorelli K,Blum M,Kwee L,Lacy Edoi
10.1016/0888-7543(92)90216-fsubject
Has Abstractpub_date
1992-05-01 00:00:00pages
159-66issue
1eissn
0888-7543issn
1089-8646pii
0888-7543(92)90216-Fjournal_volume
13pub_type
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