Abstract:
:The imprinted mouse H19 gene exhibits maternal allele-specific expression and paternal allele-specific hypermethylation. We previously demonstrated that a 14-kb H19 minitransgene possessing 5' differentially methylated sequence recapitulates the endogenous H19 imprinting pattern when present as high-copy arrays. To investigate the minimal sequences that are sufficient for H19 transgene imprinting, we have tested new transgenes in mice. While transgenes harboring limited or no 3' H19 sequence indicate that multiple elements within the 8-kb 3' fragment are required for appropriate imprinting, transgenes incorporating 1.7 kb of additional 5' sequence mimic the endogenous H19 pattern, including proper imprinting of low-copy arrays. One of these imprinted lines had a single 15.7-kb transgene integrant. This is the smallest H19 transgene identified thus far to display imprinting properties characteristic of the endogenous gene, suggesting that all cis-acting elements required for H19 imprinting in endodermal tissues reside within the 15.7-kb transgenic sequence.
journal_name
Genomicsjournal_title
Genomicsauthors
Cranston MJ,Spinka TL,Elson DA,Bartolomei MSdoi
10.1006/geno.2001.6514subject
Has Abstractpub_date
2001-04-01 00:00:00pages
98-107issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(01)96514-0journal_volume
73pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The promoter is a regulatory DNA region and important for gene transcriptional regulation. It is located near the transcription start site (TSS) upstream of the corresponding gene. In the post-genomics era, the availability of data makes it possible to build computational models for robustly detecting the promoters as...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.08.009
更新日期:2020-03-01 00:00:00
abstract::Chromosomal region 1p36.2 harbors an intriguing gene cluster of about 1 Mb. In addition to normal high-copy-number repeats, this cluster consists entirely of locally repeated sequences among which there are tRNA and small nuclear RNA (snRNA) genes. In 23 PACs and YACs from the 1p36.2 cluster, we identified eight diffe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5972
更新日期:1999-11-15 00:00:00
abstract::The Drosophila melanogaster small optic lobes gene (sol) is required for normal development of the neuropiles of the medulla and lobula complexes of the adult optic lobes. The predicted protein products of sol and its human homologue SOLH contain zinc-finger-like repeats, a calpain-like protease domain, and a C-termin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6098
更新日期:2000-02-15 00:00:00
abstract::Despite the broad variety of available microRNA (miRNA) prediction tools, their application to the discovery and annotation of novel miRNA genes in domestic species is still limited. In this study we designed a comprehensive pipeline (eMIRNA) for miRNA identification in the yet poorly annotated porcine genome and demo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.12.005
更新日期:2020-05-01 00:00:00
abstract::SHIP2 is a new member of the inositol polyphosphate 5-phosphatase family showing homology to SHIP1. The structure of both enzymes is characterized by the presence of a 5' SH2 domain, a central catalytic domain, and a 3' proline-rich region. Recent results suggest that SHIP2 and SHIP1 act downstream of various receptor...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5995
更新日期:1999-12-01 00:00:00
abstract::Evi9, a common site of retroviral integration in BXH2 murine myeloid leukemias, encodes a C2H2 zinc finger protein and is overexpressed in these leukemic cells. To investigate a possible role of EVI9 in the human hematopoietic system, we isolated the cDNA clone of the human homologue. Human EVI9, located on the chromo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6385
更新日期:2000-12-15 00:00:00
abstract::We have isolated and examined the gene for the heart isoform of cytochrome c oxidase subunit VIIa (COX VIIa-H) in mouse, an isoform gene previously thought to be lacking in rodents. Interspecies amino acid comparisons indicate that mouse COX VIIa-H protein displays 82.5 and 70.9% identity with the bovine and human hea...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5279
更新日期:1998-05-01 00:00:00
abstract::One of the important goals in systems biology is to infer transcription network based on gene expression data. Validation of the reconstructed network often requires benchmark datasets, e.g. gene expression data, which are usually unattainable. Synthetic datasets are therefore often needed to test the structure learni...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.08.009
更新日期:2009-11-01 00:00:00
abstract::Ten microsatellite DNA polymorphisms located on human chromosome 10 were regionally mapped using subchromosomal somatic cell hybrids and linkage analysis. The resulting order of the markers from pter-qter was [D10S89, D10S111], D10S107, D10S109, [D10S91, D10S110, D10S108, D10S88, D10S168], and D10S169. Order of the ma...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90455-2
更新日期:1992-03-01 00:00:00
abstract::We conducted a comprehensive analysis of codon usage bias (CUB) based on the available non-redundant full-length cDNA (nrFLcDNA) and expressed sequence tags (ESTs) data of cultivar Micro-Tom and evaluated the associations of observed CUB and measurements of transcriptional and translational effectiveness. The analysis...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.02.008
更新日期:2013-05-01 00:00:00
abstract::Creating transgenic mice is an important technology for genetic studies and is currently performed by pronuclear microinjection of plasmid DNA into fertilized eggs. Since survival of injected embryos and integration of plasmid DNA are not efficient, total efficiency is only around 3% with a standard protocol. To circu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.02.006
更新日期:2010-05-01 00:00:00
abstract::We have identified a novel human gene, designated C1orf10, using modified differential display PCR. The C1orf10 gene, which spans 5 kb in length, is composed of three exons. The deduced protein contains 495 amino acids with one transmembrane domain. The amino acid sequence of C1orf10 is characterized by the presence o...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6344
更新日期:2000-11-01 00:00:00
abstract::Williams syndrome (WS) is a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Hemizygosity of the elastin (ELN) gene can account for the vascular and connective tissue abnormalities observed in WS patients, but the genes that contribute to features such as infantile hypercalcemi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0469
更新日期:1996-09-01 00:00:00
abstract::A NotI end clone library has been constructed from a human-hamster hybrid cell line containing only human chromosome 11. Fifty-one NotI clones were chosen to characterize the library. The majority of NotI clones hybridize to small 15- to 200-kb fragments and have proven to be valuable for chromosome 11 physical mappin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1120
更新日期:1993-03-01 00:00:00
abstract::A linkage analysis with chromosome 9 markers was performed in 33 families with Friedreich ataxia (FA). Linkage with D9S15, previously established by S. Chamberlain et al. (1988, Nature London 334:248-249) was confirmed in our sample (z(theta) = 6.82 at theta = 0.02) while INFB (interferon-beta gene) shows looser linka...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90323-6
更新日期:1989-01-01 00:00:00
abstract::A large insert canine genomic bacterial artificial chromosome (BAC) library was built from a Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density hybridization filters. Insert analysis of randomly selected clones indicated a mean insert size of 155 kb and predicted 8.1 coverage of the cani...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5772
更新日期:1999-05-15 00:00:00
abstract::We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The recepto...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9996
更新日期:1995-09-20 00:00:00
abstract::Type XI collagen, a minor structural component of cartilage fibrils, is composed of three chains, alpha 1(XI), alpha 2(XI), and alpha 3(XI). Using a cloned fragment of the human alpha 2(XI) collagen gene (COL11A2) as a molecular probe for in situ hybridization and somatic cell hybrid mapping, we have localized the gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90135-3
更新日期:1989-11-01 00:00:00
abstract::The WD-repeat protein family consists of a large group of structurally related yet functionally diverse proteins found predominantly in eukaryotic cells. These factors contain several (4-16) copies of a recognizable amino-acid sequence motif (the WD unit) thought to be organized into a "propeller-like" structure invol...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6682
更新日期:2002-01-01 00:00:00
abstract::A linkage analysis with 12 DNA markers from proximal 19q was performed in eight families with central core disease (CCO). Two-point analysis gave a peak lod score of Z = 4.95 at theta = 0.00 for the anonymous marker D19S190 and of Z = 2.53 at theta = 0.00 for the ryanodine receptor (RYR1) candidate gene. Multipoint li...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1302
更新日期:1993-07-01 00:00:00
abstract::The human genome contains a group of gene families whose members map within the same regions of chromosomes 1, 6, and 9. The number of gene families involved and their pronounced clustering to the same areas of the genome indicate that their mapping relationship is nonrandom. By combining mapping data and sequence inf...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0328
更新日期:1996-07-01 00:00:00
abstract::We have established PCR assays for the genes coding for the major proteins of the desmosome type of cell junction, the desmosomal cadherins DGI (desmoglein) and DGII/III (desmocollins), and the plaque proteins DPI/II (desmoplakin) and DPIII (plakoglobin) and used them to test human-mouse and human-rat somatic cell hyb...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90446-l
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:Renal fibrosis is a final common pathway of chronic kidney disease. SIRT1, a NAD+-dependent protein deacetylase, deacetylates the p65 of NF-κB and shows protective effects in kidney disorders. miR-373 directly targets the 3'UTR of SIRT1. However, roles of miR-373 in renal fibrosis are unclear. METHODS:TGF-β...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.04.017
更新日期:2019-07-01 00:00:00
abstract::We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e, and spanning the quaking (qk) region. We describe six new probes distributed across 1.2 Mb: D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and D17Leh507. Probes D17Leh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1227
更新日期:1994-05-01 00:00:00
abstract:AIM:The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control. METHOD:The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spinal cord i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.018
更新日期:2020-09-01 00:00:00
abstract::Hemizygous deletions on chromosome 22q11.2 result in developmental disorders referred to as DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS). We report the isolation of a novel gene, PCQAP (PC2 glutamine/Q-rich-associated protein), that maps to the DiGeorge typically deleted region and encodes a protein identi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6566
更新日期:2001-06-15 00:00:00
abstract::Positional cloning strategies for the hemochromatosis gene have previously concentrated on a target area restricted to a maximum genomic expanse of 400 kb around the HLA-A and HLA-F loci. Recently, the candidate region has been extended to 2-3 Mb on the distal side of the MHC. In this study, 10 coding sequences [hemoc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0110
更新日期:1996-03-01 00:00:00
abstract::The presence of an extra copy of human chromosome 21 (trisomy 21), especially region 21q22.2, causes many phenotypes in Down syndrome, including mental retardation. To study genes potentially responsible for some of these phenotypes, we cloned a human candidate gene (DYRK) from 21q22.2 and its murine counterpart (Dyrk...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0636
更新日期:1996-12-15 00:00:00
abstract::The ky mouse mutant, kyphoscoliosis, exhibits a degenerative muscle disease resulting in chronic deformation of the spinal column. Using an interspecific backcross segregating the ky mutation, we have mapped the ky locus to a small region of mouse chromosome 9. ky is nonrecombinant with the microsatellites D9Mit24 and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80127-8
更新日期:1995-01-01 00:00:00
abstract::Members of the apolipoprotein gene cluster (APOA1/C3/A4/A5) on human chromosome 11q23 play an important role in lipid metabolism. Polymorphisms in both APOA5 and APOC3 are strongly associated with plasma triglyceride concentrations. The close genomic locations of these two genes as well as their functional similarity ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.11.016
更新日期:2004-05-01 00:00:00