Abstract:
:Calcyclin is a member of the S100 family of proteins, many of which are encoded by genes that have been localized to the proximal long arm of human chromosome 1 (bands q21-q22). A 450-kb yeast artificial chromosome clone containing the human calcyclin gene was identified by PCR screening and used as a probe for fluorescence in situ hybridization (FISH). Along with the expected hybridization to 1q21, simultaneous, specific hybridization to the centromeric region of the short arm of chromosome 1 was also observed. An identical pattern of hybridization was observed when microdissected 1q21 DNA sequences were used as a probe for FISH, confirming the presence of homologous sequences flanking both sides of the centromere of human chromosome 1. These results are consistent with a model in which human chromosome 1 arose by insertion of the centromere and heterochromatin into an ancestral chromosome containing chromosome-specific repetitive sequences.
journal_name
Genomicsjournal_title
Genomicsauthors
Hardas BD,Zhang J,Trent JM,Elder JTdoi
10.1006/geno.1994.1277subject
Has Abstractpub_date
1994-05-15 00:00:00pages
359-63issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(84)71277-8journal_volume
21pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Cataract Tohoku (Cat(Tohm)) is a dominant cataract mutation that leads to severe degeneration of lens fiber cells. Linkage analysis showed that the Cat(Tohm) mutation is located on mouse chromosome 10, close to the gene for aquaporin-0 (Aqp0), which encodes a membrane protein that is expressed specifically in lens fib...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00029-6
更新日期:2003-04-01 00:00:00
abstract::A computational and in silico system level framework was developed to identify and prioritize the antibacterial drug targets in Clostridium botulinum (Clb), the causative agent of flaccid paralysis in humans that can be fatal in 5 to 10% of cases. This disease is difficult to control due to the emergence of drug-resis...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.05.002
更新日期:2014-07-01 00:00:00
abstract::A variety of cellular proteins have been found to bind to related DNA sequences in the enhancer elements of the human immunodeficiency virus, the kappa immunoglobulin gene, the class I major histocompatibility complex gene, and the beta-interferon gene. Recently, lambda gt11 gene expression cloning using ligated oligo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90371-k
更新日期:1991-04-01 00:00:00
abstract::The transforming growth factor beta (TGF-beta) superfamily is a family of multifunctional cytokines that transduce signals via serine/threonine kinase receptors. Recent studies revealed that Mothers against dpp (Mad) in Drosophila and its homologs play important roles in the intracellular signal transduction of the se...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5149
更新日期:1998-02-15 00:00:00
abstract::Neurofibromatosis type 2 (NF2) is a dominantly inherited disease characterized by the development of bilateral vestibular schwannomas and meningiomas, which together represent 30% of primary brain tumors. The NF2 gene, which has recently been isolated, maps to the long arm of human chromosome 22. Using recombinant inb...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1291
更新日期:1994-05-15 00:00:00
abstract::Knowledge of protein subcellular localization is vitally important for both basic research and drug development. With the avalanche of protein sequences emerging in the post-genomic age, it is highly desired to develop computational tools for timely and effectively identifying their subcellular localization purely bas...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.05.017
更新日期:2019-07-01 00:00:00
abstract::We have isolated and examined the gene for the heart isoform of cytochrome c oxidase subunit VIIa (COX VIIa-H) in mouse, an isoform gene previously thought to be lacking in rodents. Interspecies amino acid comparisons indicate that mouse COX VIIa-H protein displays 82.5 and 70.9% identity with the bovine and human hea...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5279
更新日期:1998-05-01 00:00:00
abstract::We have isolated and characterized a novel cDNA coding for a highly hydrophobic protein (B5) from a fetal mouse mandibular condyle cDNA library. The full-length mouse B5 cDNA is 3095 nucleotides long and contains a potential open reading frame coding for a protein of 705 amino acids with a calculated molecular weight ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0051
更新日期:1996-02-01 00:00:00
abstract::Specific language impairment (SLI) is defined as failure to acquire normal language skills despite adequate intelligence and environmental stimulation. Although SLI disorders are often heritable, the genetic basis is likely to involve a number of risk factors. This study describes a 7-year-old girl carrying an inherit...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.04.002
更新日期:2005-08-01 00:00:00
abstract::Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80110-7
更新日期:1992-12-01 00:00:00
abstract::End sequences from bacterial artificial chromosomes (BACs) provide highly specific sequence markers in large-scale sequencing projects. To date, we have generated >300,000 end sequences from >186,000 human BAC clones with an average read length of >460 bp for a total of 141 Mb covering approximately 4.7% of the genome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6082
更新日期:2000-02-01 00:00:00
abstract::A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80008-a
更新日期:1995-02-10 00:00:00
abstract::This study presented 96 cox1 and 76 cox3 genes of Amphioctopus neglectus populations. Three distinct lineages were formed from phylogenetic trees and networks constructed using haplotypes. Mitogenomes of A. neglectus-a and A. neglectus-b as the representatives of two lineages separated from population genetics were se...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.036
更新日期:2020-11-01 00:00:00
abstract::The molecular characterization of two patients with features of Beckwith-Wiedemann syndrome (BWS) and chromosome abnormalities is consistent with the association of this phenotype with a duplication of a portion of chromosome 11. Quantitative Southern blot analysis of DNA from patient A defines a large inherited dupli...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90256-t
更新日期:1990-12-01 00:00:00
abstract::Differences in the methylation status of certain cytosine residues between active and inactive X chromosomes can be used to determine X-inactivation in females heterozygous for X-linked restriction fragment length polymorphisms. We have studied methylation patterns in 105 females heterozygous at the DXS255 locus by So...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80285-x
更新日期:1992-09-01 00:00:00
abstract::We have established a method for amplifying and obtaining large quantities of chromosome-specific DNA by linker/adaptor ligation and polymerase chain reaction (PCR). Small quantities of DNA isolated from flow cytometry-sorted chromosomes 17 and 21 were digested with MboI, ligated to a linker/adaptor, and then subjecte...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90378-6
更新日期:1992-02-01 00:00:00
abstract::The objective of this study was to identify gene expression differences in blood differences in children with autism (AU) and autism spectrum disorder (ASD) compared to general population controls. Transcriptional profiles were compared with age- and gender-matched, typically developing children from the general popul...
journal_title:Genomics
pub_type: 杂志文章,多中心研究
doi:10.1016/j.ygeno.2007.09.003
更新日期:2008-01-01 00:00:00
abstract::The vast majority of small-deletion syndromes are caused by haploinsufficiency of one or several genes and are transmitted as dominant traits. We have previously identified a homozygous deletion of 179,311 bp on chromosome 2p21 as the cause of a unique syndrome, inherited in a recessive mode, consisting of cystinuria,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.04.001
更新日期:2005-08-01 00:00:00
abstract::Androcam is a Drosophila melanogaster calmodulin-related protein that functions specifically in the testis. We show that the Acam gene is part of a cluster of three intronless genes arranged in a head-to-tail manner. The additional genes also encode calmodulin-related proteins with testis-specific transcription. Acam ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.04.009
更新日期:2006-09-01 00:00:00
abstract::The EWS gene has been identified based on its location at the chromosome 22 breakpoint of the t(11;22)(q24;q12) translocation that characterizes Ewing sarcoma and related neuroectodermal tumors. The EWS gene spans about 40 kb of DNA and is encoded by 17 exons. The nucleotide sequence of the exons is identical to that ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80363-5
更新日期:1993-12-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a heterogeneous genetic disorder with autosomal dominant, autosomal recessive, and X-linked forms. We previously mapped an additional arRP locus to chromosome 6p21 (RP14) in a single extended kinship from the Dominican Republic. Aided by a second linked RP pedigree from the same region of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5174
更新日期:1998-03-01 00:00:00
abstract::We completed a systematic survey of the human genome, conducted at an average resolution of 10 cM, for the identification of simple sequence tandem repeat polymorphisms (SSTRPs) that target new risk genes for Alzheimer disease (AD) by virtue of linkage disequilibrium. The efficiency of our association study was enhanc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5306
更新日期:1998-06-01 00:00:00
abstract::To identify genes important for taste receptor cell function, we analyzed the sequences and expression patterns of clones isolated from a mouse taste receptor cell-enriched cDNA library. Here, we report the analyses of two novel genes, Gpr113 and Trcg1. Gpr113 encodes a G-protein-coupled receptor belonging to family 2...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.12.005
更新日期:2005-04-01 00:00:00
abstract::Odorant receptors (ORs) and vomeronasal receptors (V1Rs and V2Rs) are large superfamilies of chemosensory receptors. As an extension of previous research using the 2001 Celera mouse genome assembly, we analyzed OR and V1R genes in the 2002 public mouse genome assembly. We identified 1403 OR genes (1068 potentially int...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.10.009
更新日期:2004-05-01 00:00:00
abstract::Neurturin is a potent neurotrophic factor closely related to glial cell line-derived neurotrophic factor (GDNF, 40% amino acid sequence identity) and, like GDNF, can promote the survival of numerous neuronal populations including sympathetic, nodose, and dorsal root ganglion sensory neurons. Both neurturin and GDNF si...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4846
更新日期:1997-08-15 00:00:00
abstract::We have developed a high-information-content fingerprinting (HICF) system for bacterial artificial chromosome (BAC) clones using a Type IIS restriction endonuclease, HgaI, paired with a Type II restriction endonuclease, RsaI. In the method described, unknown five-base overhangs generated with HgaI are partially or ful...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6547
更新日期:2001-06-01 00:00:00
abstract::We describe an improved method for construction of yeast artificial-chromosome (YAC) libraries that contain large inserts of foreign DNA. The procedure consists of seven steps: (i) preparation of human DNA in agarose beads; (ii) partial digestion of the DNA with EcoRI; (iii) electrophoretic elimination of the smaller ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90285-3
更新日期:1990-10-01 00:00:00
abstract::The general strategies of phototransduction in vertebrates and invertebrates share many similarities, but differ significantly in their underlying molecular machinery. The CDS gene encodes the CDP-diacylglycerol synthase (CDS) enzyme and is required for phototransduction in Drosophila. Using a bioinformatic approach, ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5610
更新日期:1999-01-01 00:00:00
abstract::In this study, we systematically screened the polymorphisms of the whole CYP2D6 gene in the populations of four different geographical locations in China, namely, Shanghai, Shantou, Shenyang, and Xi'an, using a sample of 100 subjects from each population. Forty-eight different polymorphisms were detected as well as 12...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.05.004
更新日期:2008-09-01 00:00:00
abstract::The promoter is a regulatory DNA region and important for gene transcriptional regulation. It is located near the transcription start site (TSS) upstream of the corresponding gene. In the post-genomics era, the availability of data makes it possible to build computational models for robustly detecting the promoters as...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.08.009
更新日期:2020-03-01 00:00:00