Abstract:
:We have determined the cDNA and genomic structure of a gene (-14 gene) that lies adjacent to the human alpha-globin cluster. Although it is expressed in a wide range of cell lines and tissues, a previously described erythroid-specific regulatory element that controls expression of the alpha-globin genes lies within intron 5 of this gene. Analysis of the -14 gene promoter shows that it is GC rich and associated with a constitutively expressed DNase 1 hypersensitive site; unlike the alpha-globin promoter, it does not contain a TATA or CCAAT box. These and other differences in promoter structure may explain why the erythroid regulatory element interacts specifically with the alpha-globin promoters and not the -14 gene promoter, which lies between the alpha promoters and their regulatory element. Interspecies comparisons demonstrate that the sequence and location of the -14 gene adjacent to the alpha cluster have been maintained since the bird/mammal divergence, 270 million years ago.
journal_name
Genomicsjournal_title
Genomicsauthors
Vyas P,Vickers MA,Picketts DJ,Higgs DRdoi
10.1006/geno.1995.9951subject
Has Abstractpub_date
1995-10-10 00:00:00pages
679-89issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(85)79951-Xjournal_volume
29pub_type
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